Biology:Mimosinase
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Mimosinase (EC 4.3.3.8) is an enzyme characterised from Leucaena leaves that catalyzes the overall chemical reaction which converts mimosine to 3-hydroxypyridin-4(1H)-one (a tautomer of 3,4-dihydroxypyridine), pyruvic acid, and ammonia:[1][2]
- REDIRECT Template:Chemical reaction
The enzyme contains pyridoxal phosphate and the first step of the reaction breaks the bond between the pyridinone nitrogen and the sidechain, which at first forms an unstable enamine derivative. This is then hydrolysed to pyruvic acid and ammonia.[3][4][5]
This enzyme is a carbon-nitrogen lyase.[6]
Occurrence
Known to occur in all Leucaena and Mimosa species of plant. The synthase found in L. leucocephala has been cloned.[7]
References
- ↑ "Isolation of a mimosine degrading enzyme from Leucaena leaf". J. Sci. Food Agric. 37 (6): 523–526. 1986. doi:10.1002/jsfa.2740370603. Bibcode: 1986JSFA...37..523T.
- ↑ Nguyen, Binh Cao Quan; Tawata, Shinkichi (2016). "The Chemistry and Biological Activities of Mimosine: A Review". Phytotherapy Research 30 (8): 1230–1242. doi:10.1002/ptr.5636. PMID 27213712.
- ↑ Negi, Vishal Singh; Bingham, Jon-Paul; Li, Qing X.; Borthakur, Dulal (2014). "A Carbon-Nitrogen Lyase from Leucaena leucocephala Catalyzes the First Step of Mimosine Degradation". Plant Physiology 164 (2): 922–934. doi:10.1104/pp.113.230870. PMID 24351687.
- ↑ Oogai, Shigeki; Fukuta, Masakazu; Watanabe, Keiichi; Inafuku, Masashi; Oku, Hirosuke (2019). "Molecular characterization of mimosinase and cystathionine β-lyase in the Mimosoideae subfamily member Mimosa pudica". Journal of Plant Research 132 (5): 667–680. doi:10.1007/s10265-019-01128-4. PMID 31368041.
- ↑ Oogai, Shigeki; Fukuta, Masakazu; Inafuku, Masashi; Oku, Hirosuke (2022). "Isolation and characterization of mimosine degrading enzyme from Arthrobacter sp. Ryudai-S1". World Journal of Microbiology and Biotechnology 38 (10). doi:10.1007/s11274-022-03344-y. PMID 35908235. https://u-ryukyu.repo.nii.ac.jp/records/2020878.
- ↑ Enzyme 4.3.3.8 at KEGG Pathway Database.
- ↑ Negi, V.S.; Borthakur, D. (2016). "Heterologous Expression and Characterization of Mimosinase from Leucaena leucocephala". Biotechnology of Plant Secondary Metabolism : Methods and Protocols. Methods in Molecular Biology (MIMB). 1405. New York, NY: Springer Science+Business Media. pp. 59–77. doi:10.1007/978-1-4939-3393-8. ISBN 978-1-4939-3391-4.
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