Biology:Α,α-Trehalase

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α,α-Trehalase
Identifiers
EC number3.2.1.28
CAS number9025-52-9
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO

An α,α-trehalase (EC 3.2.1.28) is an enzyme with systematic name α,α-trehalose glucohydrolase.[1][2][3][4] This enzyme catalyzes the chemical reaction

α,α-trehalose + H2O [math]\displaystyle{ \rightleftharpoons }[/math] 2 D-glucose

This enzyme belongs to the family of hydrolases, specifically those glycosidases that hydrolyse O- and S-glycosyl compounds. It is also called trehalase, and it participates in starch and sucrose metabolism.

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 2JF4 and 2JG0.

References

  1. "Trehalose und Hefe. II. Trehalasewirkung von Hefepräparaten". Biochem. Z. 291: 61–69. 1937. 
  2. "The purification and properties of trehalase". The Journal of Biological Chemistry 230 (2): 691–8. February 1958. PMID 13525386. 
  3. "Trehalase: stereocomplementary hydrolytic and glucosyl transfer reactions with α- and β-D-glucosyl fluoride". Biochemistry 21 (13): 3090–7. June 1982. doi:10.1021/bi00256a009. PMID 7104311. 
  4. "Catalytic reaction mechanism based on α-secondary deuterium isotope effects in hydrolysis of trehalose by European honeybee trehalase". Bioscience, Biotechnology, and Biochemistry 73 (11): 2466–73. November 2009. doi:10.1271/bbb.90447. PMID 19897915.