Biology:Cytosine deaminase
| cytosine deaminase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 3.5.4.1 | ||||||||
| CAS number | 9025-05-2 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
In enzymology, a cytosine deaminase (EC 3.5.4.1) is an enzyme that catalyzes the chemical reaction
- cytosine + H2O uracil + NH3
Thus, the two substrates of this enzyme are cytosine[1] and H2O, whereas its two products are uracil and NH3.
This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in cyclic amidines. The systematic name of this enzyme class is cytosine aminohydrolase. This enzyme is also called isocytosine deaminase. This enzyme participates in pyrimidine metabolism.
The non-mammalian enzyme is known to mediate 5-fluorcytosine (5-FC) an antifungal medication prodrug into 5-fluorouracil (5-FU) a cytotoxic chemotherapy drug.[2]
Structural studies
As of late 2007, 13 structures have been solved for this class of enzymes, with PDB accession codes 1K6W, 1K70, 1OX7, 1P6O, 1R9X, 1R9Y, 1RA0, 1RA5, 1RAK, 1RB7, 1UAQ, 1YSB, and 1YSD.
References
- ↑ Sukumar, Uday Kumar; Packirisamy, Gopinath (2015-08-11). "Bioactive Core–Shell Nanofiber Hybrid Scaffold for Efficient Suicide Gene Transfection and Subsequent Time Resolved Delivery of Prodrug for Anticancer Therapy" (in EN). ACS Applied Materials & Interfaces 7 (33): 18717–18731. doi:10.1021/acsami.5b05280. ISSN 1944-8244. PMID 26234345.
- ↑ El-Sayed, Ashraf S.A.; Mohamed, Nabil Z.; Yassin, Marwa A.; Amer, Mahmoud M.; El-Sharkawy, Reyad; El-Sayed, Nesma; Ali, Mostafa G. (September 2022). "Microbial cytosine deaminase is a programmable anticancer prodrug mediating enzyme: antibody, and gene directed enzyme prodrug therapy" (in en). Heliyon 8 (9): e10660. doi:10.1016/j.heliyon.2022.e10660. PMID 36164544. PMC 9508425. https://linkinghub.elsevier.com/retrieve/pii/S240584402201948X.
- "The conversion of 5-methyldeoxycytidine to thymidine in vitro and in vivo". J. Biol. Chem. 226 (2): 631–42. 1957. doi:10.1016/S0021-9258(18)70845-4. PMID 13438848.
- Kream J; Chargaff E (1952). "On the cytosine deaminase of yeast". J. Am. Chem. Soc. 74 (20): 5157–5160. doi:10.1021/ja01140a050. Bibcode: 1952JAChS..74.5157K.
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