Biology:Gellan tetrasaccharide unsaturated glucuronyl hydrolase

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Gellan tetrasaccharide unsaturated glucuronyl hydrolase
Identifiers
EC number3.2.1.179
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum

Gellan tetrasaccharide unsaturated glucuronyl hydrolase (EC 3.2.1.179, UGL, unsaturated glucuronyl hydrolase) is an enzyme with systematic name beta-D-4-deoxy-Delta4-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp beta-D-4-deoxy-Delta4-GlcAp hydrolase.[1][2][3] This enzyme catalyses the following chemical reaction

beta-D-4-deoxy-Delta4-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp + H2O [math]\displaystyle{ \rightleftharpoons }[/math] 5-dehydro-4-deoxy-D-glucuronate + beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp

The enzyme releases 4-deoxy-4(5)-unsaturated D-glucuronic acid from oligosaccharides produced by polysaccharide lyases.

References

  1. "Crystal structure of unsaturated glucuronyl hydrolase, responsible for the degradation of glycosaminoglycan, from Bacillus sp. GL1 at 1.8 A resolution". The Journal of Biological Chemistry 279 (30): 31804–12. July 2004. doi:10.1074/jbc.m403288200. PMID 15148314. 
  2. "Unsaturated glucuronyl hydrolase of Bacillus sp. GL1: novel enzyme prerequisite for metabolism of unsaturated oligosaccharides produced by polysaccharide lyases". Archives of Biochemistry and Biophysics 368 (2): 367–74. August 1999. doi:10.1006/abbi.1999.1305. PMID 10441389. 
  3. "Substrate recognition by unsaturated glucuronyl hydrolase from Bacillus sp. GL1". Biochemical and Biophysical Research Communications 344 (1): 253–62. May 2006. doi:10.1016/j.bbrc.2006.03.141. PMID 16630576. 

External links