Biology:Plastid terminal oxidase

From HandWiki

Plastid terminal oxidase or plastoquinol terminal oxidase (PTOX) is an enzyme that resides on the thylakoid membranes of plant and algae chloroplasts and on the membranes of cyanobacteria. The enzyme was hypothesized to exist as a photosynthetic oxidase in 1982 and was verified by sequence similarity to the mitochondrial alternative oxidase (AOX).[1] The two oxidases evolved from a common ancestral protein in prokaryotes, and they are so functionally and structurally similar that a thylakoid-localized AOX can restore the function of a PTOX knockout.[2]

Function

Plastid terminal oxidase catalyzes the oxidation of the plastoquinone pool, which exerts a variety of effects on the development and functioning of plant chloroplasts.

A summary of the pathways plastid terminal oxidase plays a role in through oxidation of the quinone pool

Carotenoid biosynthesis and plastid development

The enzyme is important for carotenoid biosynthesis during chloroplast biogenesis. In developing plastids, its activity prevents the over-reduction of the plastoquinone pool. Knockout plants for PTOX exhibit phenotypes of variegated leaves with white patches. Without the enzyme, the carotenoid synthesis pathway slows down due to the lack of oxidized plastoquinone with which to oxidize phytoene, a carotenoid intermediate. The colorless compound phytoene accumulates in the leaves, resulting in white patches of cells.[3] PTOX is also thought to determine the redox poise of the developing photosynthetic apparatus and without it, plants fail to assemble organized internal membrane structures in chloroplasts when exposed to high light during early development.[1][4]

Photoprotection

Plants deficient in the IMMUTANS gene that encodes the oxidase are especially susceptible to photooxidative stress during early plastid development. The knockout plants exhibit a phenotype of variegated leaves with white patches that indicate a lack of pigmentation or photodamage. This effect is enhanced with increased light and temperature during plant development. The lack of plastid terminal oxidase indirectly causes photodamage during plastid development because protective carotenoids are not synthesized without the oxidase.[5]

The enzyme is also thought to act as a safety valve for stress conditions in the photosynthetic apparatus. By providing an electron sink when the plastoquinone pool is over-reduced, the oxidase is thought to protect photosystem II from oxidative damage. Knockouts for Rubisco and photosystem II complexes, which would experience more photodamage than normal, exhibit an upregulation of plastid terminal oxidase.[6] This effect is not universal because it requires plants to have additional PTOX regulation mechanisms. While many studies agree with the stress-protective role of the enzyme, one study showed that overexpression of PTOX increases the production of reactive oxygen species and causes more photodamage than normal. This finding suggests that an efficient antioxidant system is required for the oxidase to function as a safety valve for stress conditions and that it is more important during chloroplast biogenesis than in the regular functioning of the chloroplast.[7]

Chlororespiration and electron flux

The most confirmed function of plastid terminal oxidase in developed chloroplasts is its role in chlororespiration. In this process, NADPH dehydrogenase (NDH) reduces the quinone pool and the terminal oxidase oxidizes it, serving the same function as cytochrome c oxidase from mitochondrial electron transport. In Chlamydomonas, there are two copies of the gene for the oxidase. PTOX2 significantly contributes to the flux of electrons through chlororespiration in the dark.[8] There is also evidence from experiments with tobacco that it functions in plant chlororespiration as well.[9]

In fully developed chloroplasts, prolonged exposure to light increases the activity of the oxidase. Because the enzyme acts at the plastoquinone pool in between photosystem II and photosystem I, it may play a role in controlling electron flow through photosynthesis by acting as an alternative electron sink. Similar to its role in carotenoid synthesis, its oxidase activity may prevent the over-reduction of photosystem I electron acceptors and damage by photoinhibition. A recent analysis of electron flux through the photosynthetic pathway shows that even when activated, the electron flux plastid terminal oxidase diverts is two orders of magnitude less than the total flux through photosynthetic electron transport.[10] This suggests that the protein may play less of a role than previously thought in relieving the oxidative stress in photosynthesis.

Structure

Plastid terminal oxidase is an integral membrane protein, or more specifically, an integral monotopic protein and is bound to the thylakoid membrane facing the stroma. Based on sequence homology, the enzyme is predicted to contain four alpha helix domains that encapsulate a di-iron center. The two iron atoms are ligated by six essential conserved histidine and glutamate residues – Glu136, Glu175, His171, Glu227, Glu296, and His299.[11] The predicted structure is similar to that of the alternative oxidase, with an additional Exon 8 domain that is required for the plastid oxidase's activity and stability. The enzyme is anchored to the membrane by a short fifth alpha helix that contains a Tyr212 residue hypothesized to be involved in substrate binding.[12]

Mechanism

The oxidase catalyzes the transfer of four electrons from reduced plastoquinone to molecular oxygen to form water . The net reaction is written below:

2 QH2 + O2 → 2 Q + 2 H2O

Analysis of substrate specificity revealed that the enzyme almost exclusively catalyzes the reduction of plastoquinone over other quinones such as ubiquinone and duroquinone. Additionally, iron is essential for the catalytic function of the enzyme and cannot be substituted by another metal cation like Cu2+, Zn2+, or Mn2+ at the catalytic center.[13]

It is unlikely that four electrons could be transferred at once in a single iron cluster, so all of the proposed mechanisms involve two separate two-electron transfers from reduced plastoquinone to the di-iron center. In the first step common to all proposed mechanisms, one plastoquinone is oxidized and both irons are reduced from iron(III) to iron(II). Four different mechanisms are proposed for the next step, oxygen capture. One mechanism proposes a peroxide intermediate, after which one oxygen atom is used to create water and another is left bound in a diferryl configuration. Upon one more plastoquinone oxidation, a second water molecule is formed and the irons return to a +3 oxidation state. The other mechanisms involve the formation of Fe(III)-OH or Fe(IV)-OH and a tyrosine radical.[14] These radical-based mechanisms could explain why over-expression of the PTOX gene causes increased generation of reactive oxygen species.

Evolution

The enzyme is present in organisms capable of oxygenic photosynthesis, which includes plants, algae, and cyanobacteria. Plastid terminal oxidase and alternative oxidase are thought to have originated from a common ancestral di-iron carboxylate protein. Oxygen reductase activity was likely an ancient mechanism to scavenge oxygen in the early transition from an anaerobic to aerobic world. The plastid oxidase first evolved in ancient cyanobacteria and the alternative oxidase in Pseudomonadota before eukaryotic evolution and endosymbiosis events. Through endosymbiosis, the plastid oxidase was vertically inherited by eukaryotes that evolved into plants and algae. Sequenced genomes of various plant and algae species shows that the amino acid sequence is more than 25% conserved, which is a significant amount of conservation for an oxidase. This sequence conservation further supports the theory that both the alternative and plastid oxidases evolved before endosymbiosis and did not significantly change through eukaryote evolution.[15]

There also exist PTOX cyanophages that contain copies of the gene for the plastid oxidase. They are known to act as viral vectors for movement of the gene between cyanobacterial species. Some evidence suggests that the phages may use the oxidase to influence photosynthetic electron flow to produce more ATP and less NADPH because viral synthesis utilizes more ATP.[1]

See also

References

  1. ↑ 1.0 1.1 1.2 "Flexibility in photosynthetic electron transport: the physiological role of plastoquinol terminal oxidase (PTOX)". Biochim. Biophys. Acta 1807 (8): 954–67. August 2011. doi:10.1016/j.bbabio.2010.10.024. PMID 21056542. 
  2. ↑ "Alternative oxidases (AOX1a and AOX2) can functionally substitute for plastid terminal oxidase in Arabidopsis chloroplasts". Plant Cell 24 (4): 1579–95. April 2012. doi:10.1105/tpc.112.096701. PMID 22534126. 
  3. ↑ "A plastid terminal oxidase comes to light: implications for carotenoid biosynthesis and chlororespiration". Trends Plant Sci. 6 (1): 31–6. January 2001. doi:10.1016/S1360-1385(00)01811-2. PMID 11164375. 
  4. ↑ "The Mechanism of Variegation in immutans Provides Insight into Chloroplast Biogenesis". Front. Plant Sci. 3 (260): 260. November 2012. doi:10.3389/fpls.2012.00260. PMID 23205022. 
  5. ↑ "Control of chloroplast redox by the IMMUTANS terminal oxidase". Physiol. Plant. 120 (1): 4–11. January 2004. doi:10.1111/j.0031-9317.2004.0217.x. PMID 15032871. 
  6. ↑ "Physiological roles of plastid terminal oxidase in plant stress responses". J. Biosci. 36 (5): 951–6. December 2011. doi:10.1007/s12038-011-9161-7. PMID 22116293. 
  7. ↑ "Plastid alternative oxidase (PTOX) promotes oxidative stress when overexpressed in tobacco". J. Biol. Chem. 284 (45): 31174–80. November 2009. doi:10.1074/jbc.M109.021667. PMID 19740740. 
  8. ↑ "Plastid terminal oxidase 2 (PTOX2) is the major oxidase involved in chlororespiration in Chlamydomonas". PNAS 108 (51): 20820–20825. December 2011. doi:10.1073/pnas.1110518109. PMID 22143777. Bibcode: 2011PNAS..10820820H. 
  9. ↑ "Involvement of a plastid terminal oxidase in plastoquinone oxidation as evidenced by expression of the Arabidopsis thaliana enzyme in tobacco". J. Biol. Chem. 277 (35): 31623–30. August 2002. doi:10.1074/jbc.M203538200. PMID 12050159. 
  10. ↑ "Kinetic properties and physiological role of the plastoquinone terminal oxidase (PTOX) in a vascular plant". Biochim. Biophys. Acta 1817 (12): 2140–8. December 2012. doi:10.1016/j.bbabio.2012.08.006. PMID 22982477. 
  11. ↑ "Sequences required for the activity of PTOX (IMMUTANS), a plastid terminal oxidase: in vitro and in planta mutagenesis of iron-binding sites and a conserved sequence that corresponds to Exon 8". J. Biol. Chem. 280 (52): 42489–96. December 2005. doi:10.1074/jbc.M508940200. PMID 16249174. 
  12. ↑ "Conserved active site sequences in Arabidopsis plastid terminal oxidase (PTOX): in vitro and in planta mutagenesis studies". J. Biol. Chem. 284 (34): 22625–32. August 2009. doi:10.1074/jbc.M109.017905. PMID 19542226. 
  13. ↑ "In vitro characterization of a plastid terminal oxidase (PTOX)". Eur. J. Biochem. 270 (18): 3787–94. September 2003. doi:10.1046/j.1432-1033.2003.03766.x. PMID 12950262. 
  14. ↑ "Exploring the molecular nature of alternative oxidase regulation and catalysis". FEBS Lett. 510 (3): 121–6. January 2002. doi:10.1016/S0014-5793(01)03261-6. PMID 11801238. 
  15. ↑ "Origins, evolutionary history, and taxonomic distribution of alternative oxidase and plastoquinol terminal oxidase". Comp. Biochem. Physiol. C 1 (3): 357–64. September 2006. doi:10.1016/j.cbd.2006.08.001. PMID 20483267. 

External links



Retrieved from "https://handwiki.org/wiki/index.php?title=Biology:Plastid_terminal_oxidase&oldid=3609067"