Biology:Methylmalonyl-CoA mutase
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methylmalonyl-CoA mutase | |||||||||
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Identifiers | |||||||||
EC number | 5.4.99.2 | ||||||||
CAS number | 9023-90-9 | ||||||||
Databases | |||||||||
IntEnz | IntEnz view | ||||||||
BRENDA | BRENDA entry | ||||||||
ExPASy | NiceZyme view | ||||||||
KEGG | KEGG entry | ||||||||
MetaCyc | metabolic pathway | ||||||||
PRIAM | profile | ||||||||
PDB structures | RCSB PDB PDBe PDBsum | ||||||||
Gene Ontology | AmiGO / QuickGO | ||||||||
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Methylmalonyl-CoA mutase (EC 5.4.99.2, MCM), mitochondrial, also known as methylmalonyl-CoA isomerase, is a protein that in humans is encoded by the MUT gene. This vitamin B12-dependent enzyme catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA in humans. Mutations in MUT gene may lead to various types of methylmalonic aciduria.[1]
MCM was first identified in rat liver and sheep kidney in 1955. In its latent form, it is 750 amino acids in length. Upon entry to the mitochondria, the 32 amino acid mitochondrial leader sequence at the N-terminus of the protein is cleaved, forming the fully processed monomer. The monomers then associate into homodimers, and bind AdoCbl (one for each monomer active site) to form the final, active holoenzyme form.[2]
Structure
Gene
The MUT gene lies on the chromosome location of 6p12.3 and consists of 13 exons, spanning over 35kb.[3]
Protein
The mature enzyme is a homodimer with the N-terminal CoA binding domain and the C- terminal cobalamin-binding domain.[4]
Function
Methylmalonyl-CoA mutase is expressed in high concentrations in the kidney, in intermediate concentrations in the heart, ovaries, brain, muscle, and liver, and in low concentrations in the spleen.[2] The enzyme can be found all throughout the central nervous system (CNS).[2] MCM resides in the mitochondria, where a number of substances, including the branched-chain amino acids isoleucine and valine, as well as methionine, threonine, thymine and odd-chain fatty acids, are metabolized via methylmalonate semialdehyde (MMlSA) or propionyl-CoA (Pr-CoA) to a common compound - methylmalonyl-CoA (MMl-CoA). MCM catalyzes the reversible isomerisation of l‐methylmalonyl‐CoA to succinyl‐CoA, requiring cobalamin (vitamin B12) in the form of adenosylcobalamin (AdoCbl) as a cofactor. As an important step in propionate catabolism, this reaction is required for the degradation of odd-chain fatty acids, the amino acids valine, isoleucine, methionine, and threonine, and cholesterol,[5] funneling metabolites from the breakdown of these amino acids into the tricarboxylic acid cycle.[6]
Methylmalonyl-CoA mutase catalyzes the following reaction:
L-methylmalonyl-CoA | methylmalonyl-CoA mutase | Succinyl-CoA | |
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methylmalonyl-CoA mutase |
The substrate of methylmalonyl-CoA mutase, methylmalonyl-CoA, is primarily derived from propionyl-CoA, a substance formed from the catabolism and digestion of isoleucine, valine, threonine, methionine, thymine, cholesterol, or odd-chain fatty acids. The product of the enzyme, succinyl-CoA, is a key molecule of the tricarboxylic acid cycle.
Clinical significance
A deficiency of this enzyme is responsible for an inherited disorder of metabolism, methylmalonyl-CoA mutase deficiency, which is one of the causes of methylmalonic acidemia (also referred to as methylmalonic aciduria or MMA). MMA is an autosomal recessive inherited inborn error of metabolism, characterized by recurrent episodes of vomiting, lethargy, profound ketoacidosis, hyperammonemia, and pancytopenia in infancy, and may cause early death. Complications include cardiomyopathy, metabolic stroke, pancreatitis, and progressive renal failure.[1][7]
Either mutations to the gene MUT (encodes methylmalonyl-CoA mutase), or MMAA (encodes a chaperone protein of methylmalonyl-CoA mutase, MMAA protein) can lead to methylmalonyl acidemia.[8] Mutations to MUT can be categorized as either MUT0 (demonstrates no activity even in presence of excess AdoCbl), or MUT1 (demonstrates very low activity in presence of excess AdoCbl).[4] Over half of the mutations of MUT are missense mutations[6] while nonsense mutations comprise a significant remaining fraction (approximately 14%)[9]
Common treatment methods for MMA include a liver transplant or a liver and kidney transplant to combat the renal disease of methylmalonic acidemia. However, detrimental neurological effects can continue to plague patients even after a successful operation. It is thought that this is due to the widespread presence of methylmalonyl-CoA mutase throughout the central nervous system. Due to the loss of functionality of the enzyme, substrate levels build up in the CNS. The substrate, L-methylmalonyl-CoA hydrolyzes to form methylmalonate (methylmalonic acid), a neurotoxic dicarboxylic acid that, due to the poor dicarboxylic acid transport capacities of the blood-brain barrier, is effectively trapped within the CNS, leading to neurological debilitation. To combat these effects perioperative anti-catabolic regimes and no diet discontinuation are recommended.[2]
The murine model has proven an adequate and accurate way of studying the effects of MMA, and potential treatment methods.[10][11]
Mechanism
The MCM reaction mechanism begins with homolytic cleavage of AdoB12's C-Co(III) bond, the C and Co atoms each acquire one of the electrons that formed the cleaved electron pair bond. The Co ion, therefore, fluctuates between its Co(III) and Co(II) oxidation states [the two states are spectroscopically distinguishable: Co(III) is red and diamagnetic (no unpaired electrons), whereas Co(II) is yellow and paramagnetic (unpaired electrons)]. Hence, the role of coenzyme B-12 in the catalytic process is that of a reversible generator of a free radical. The C-Co(III) bond is weak, with a dissociation energy = 109 kJ/mol, and appears to be further weakened through steric interactions with the enzyme. The homolytic reaction is unusual in biology, as is the presence of a metal-carbon bond.
Methylmalonyl-CoA mutase is a member of the isomerase subfamily of adenosylcobalamin-dependent enzymes. Furthermore, it is classified as class I, as it is a ‘DMB-off’/’His-on’ enzyme. This refers to the nature of the AdoCbl cofactor in the active site of methylmalonyl CoA.[12] AdoCbl is composed of a central cobalt-containing corrin ring, an upper axial ligand (β-axial ligand), and a lower axial ligand (α-axial ligand). In methylmalonyl-CoA mutase, the β-axial ligand 5’-deoxy-5’-adenosine reversibly dissociated to give the deoxyadenosyl radical. The α-axial ligand 5,6-dimethylbenzimidazole (DMB) is involved in organizing the active site to enable histidine-610 to bond with Co, instead of DMB (the reason for the ‘DMB-off’/’His-on’ notation).[12] Binding of histidine-610 residue increases the rate of homolytic β-axial ligand – Co bond breakage by a factor of 1012.[13]
![](/wiki/images/thumb/e/e0/Active_site_MCM_colors.png/400px-Active_site_MCM_colors.png)
Other important residues of methylmalonyl-CoA mutase include Histidine-244, which acts as a general acid near the substrate and shields the radical species from side reactions involving oxygen,[16] Glutamate-370, whose hydrogen bond with the 2’-OH group of the ribose of the β-axial ligand forces interaction between the β-axial ligand radical species and the substrate,[17] and tyrosine-89 which stabilizes reactive radical intermediates and accounts for the stereo-selectivity of the enzyme.[14][18]
The processing protein, MMAA protein, fills the important role of aiding cofactor loading and exchange.[8][19] MMAA protein favors association with the MCM apoenzyme, and allows for the transfer of the AdoCbl cofactor to the enzyme active site.[19] Furthermore, if the bound AdoCbl accrues oxidative damage during normal functioning, MMAA protein fosters exchange of the damaged cofactor for a new AdoCbl via a GTP-reliant pathway.[8][19]
Interactions
References
- ↑ 1.0 1.1 "Molecular, biochemical, and structural analysis of a novel mutation in patients with methylmalonyl-CoA mutase deficiency". Gene 576 (1 Pt 2): 208–13. January 2016. doi:10.1016/j.gene.2015.10.002. PMID 26449400.
- ↑ 2.0 2.1 2.2 2.3 "Evidence for catabolic pathway of propionate metabolism in CNS: expression pattern of methylmalonyl-CoA mutase and propionyl-CoA carboxylase alpha-subunit in developing and adult rat brain". Neuroscience 164 (2): 578–87. December 2009. doi:10.1016/j.neuroscience.2009.08.028. PMID 19699272.
- ↑ "Genetic and genomic systems to study methylmalonic acidemia". Molecular Genetics and Metabolism 86 (1–2): 34–43. 2016-10-01. doi:10.1016/j.ymgme.2005.07.020. PMID 16182581.
- ↑ 4.0 4.1 "Molecular basis for dysfunction of some mutant forms of methylmalonyl-CoA mutase: deductions from the structure of methionine synthase". Proceedings of the National Academy of Sciences of the United States of America 93 (11): 5550–5. May 1996. doi:10.1073/pnas.93.11.5550. PMID 8643613. Bibcode: 1996PNAS...93.5550D.
- ↑ "Genetic analysis of three genes causing isolated methylmalonic acidemia: identification of 21 novel allelic variants". Molecular Genetics and Metabolism 84 (4): 317–25. April 2005. doi:10.1016/j.ymgme.2004.11.011. PMID 15781192.
- ↑ 6.0 6.1 "Functional characterization and categorization of missense mutations that cause methylmalonyl-CoA mutase (MUT) deficiency". Human Mutation 35 (12): 1449–58. December 2014. doi:10.1002/humu.22633. PMID 25125334.
- ↑ "Microarray based mutational analysis of patients with methylmalonic acidemia: identification of 10 novel mutations". Molecular Genetics and Metabolism 106 (4): 419–23. August 2012. doi:10.1016/j.ymgme.2012.05.014. PMID 22727635.
- ↑ 8.0 8.1 8.2 "Protection and reactivation of human methylmalonyl-CoA mutase by MMAA protein". Biochemical and Biophysical Research Communications 404 (1): 443–7. January 2011. doi:10.1016/j.bbrc.2010.11.141. PMID 21138732.
- ↑ "Treatment of a methylmalonyl-CoA mutase stopcodon mutation". Biochemical and Biophysical Research Communications 427 (4): 753–7. November 2012. doi:10.1016/j.bbrc.2012.09.133. PMID 23041189.
- ↑ "Pre-clinical efficacy and dosing of an AAV8 vector expressing human methylmalonyl-CoA mutase in a murine model of methylmalonic acidemia (MMA)". Molecular Genetics and Metabolism 107 (3): 617–9. November 2012. doi:10.1016/j.ymgme.2012.09.019. PMID 23046887.
- ↑ "Targeting proximal tubule mitochondrial dysfunction attenuates the renal disease of methylmalonic acidemia". Proceedings of the National Academy of Sciences of the United States of America 110 (33): 13552–7. August 2013. doi:10.1073/pnas.1302764110. PMID 23898205. Bibcode: 2013PNAS..11013552M.
- ↑ 12.0 12.1 "Role of vitamin B12 on methylmalonyl-CoA mutase activity". Journal of Zhejiang University Science B 13 (6): 423–37. June 2012. doi:10.1631/jzus.B1100329. PMID 22661206.
- ↑ "Importance of the histidine ligand to coenzyme B12 in the reaction catalyzed by methylmalonyl-CoA mutase". The Journal of Biological Chemistry 277 (21): 18523–7. May 2002. doi:10.1074/jbc.M111809200. PMID 11893736.
- ↑ 14.0 14.1 "Crystal structure of substrate complexes of methylmalonyl-CoA mutase". Biochemistry 38 (25): 7999–8005. June 1999. doi:10.1021/bi9903852. PMID 10387043.
- ↑ "Conformational changes on substrate binding to methylmalonyl CoA mutase and new insights into the free radical mechanism". Structure 6 (6): 711–20. June 1998. doi:10.1016/s0969-2126(98)00073-2. PMID 9655823.
- ↑ "Proton transfer from histidine 244 may facilitate the 1,2 rearrangement reaction in coenzyme B(12)-dependent methylmalonyl-CoA mutase". The Journal of Biological Chemistry 274 (46): 32733–7. November 1999. doi:10.1074/jbc.274.46.32733. PMID 10551831.
- ↑ "Hydrogen bonds guide the short-lived 5'-deoxyadenosyl radical to the place of action". Angewandte Chemie 51 (40): 9974–6. October 2012. doi:10.1002/anie.201205299. PMID 22945861.
- ↑ "Stabilization of radical intermediates by an active-site tyrosine residue in methylmalonyl-CoA mutase". Biochemistry 37 (41): 14386–93. October 1998. doi:10.1021/bi981375o. PMID 9772164.
- ↑ 19.0 19.1 19.2 19.3 19.4 "Structures of the human GTPase MMAA and vitamin B12-dependent methylmalonyl-CoA mutase and insight into their complex formation". The Journal of Biological Chemistry 285 (49): 38204–13. December 2010. doi:10.1074/jbc.M110.177717. PMID 20876572.
- ↑ "Energetics of interaction between the G-protein chaperone, MeaB, and B12-dependent methylmalonyl-CoA mutase". The Journal of Biological Chemistry 281 (26): 17838–44. June 2006. doi:10.1074/jbc.M600047200. PMID 16641088.
- ↑ "Inhibition of the human methylmalonyl-CoA mutase by various CoA-esters". The Journal of Biological Chemistry 269 (50): 31630–4. December 1994. doi:10.1016/S0021-9258(18)31741-1. PMID 7989334.
Further reading
- "Mutations in mut methylmalonic acidemia: clinical and enzymatic correlations". Human Mutation 9 (1): 1–6. 1997. doi:10.1002/(SICI)1098-1004(1997)9:1<1::AID-HUMU1>3.0.CO;2-E. PMID 8990001.
- "Structure-based perspectives on B12-dependent enzymes". Annual Review of Biochemistry 66: 269–313. 1997. doi:10.1146/annurev.biochem.66.1.269. PMID 9242908.
- "Renal transplant in methylmalonic acidemia: could it be the best option? Report on a case at 10 years and review of the literature". Pediatric Nephrology 22 (8): 1209–14. August 2007. doi:10.1007/s00467-007-0460-z. PMID 17401587.
- "Intracellular localization of hepatic propionyl-CoA carboxylase and methylmalonyl-CoA mutase in humans and normal and vitamin B12 deficient rats". British Journal of Haematology 31 (4): 501–13. December 1975. doi:10.1111/j.1365-2141.1975.tb00885.x. PMID 24458.
- "Cloning and expression of a mutant methylmalonyl coenzyme A mutase with altered cobalamin affinity that causes mut- methylmalonic aciduria". The Journal of Clinical Investigation 89 (2): 385–91. February 1992. doi:10.1172/JCI115597. PMID 1346616.
- "Phenotype of disease in three patients with identical mutations in methylmalonyl CoA mutase". Human Genetics 89 (3): 259–64. May 1992. doi:10.1007/BF00220536. PMID 1351030.
- "Genetic characterization of a MUT locus mutation discriminating heterogeneity in mut0 and mut- methylmalonic aciduria by interallelic complementation". The Journal of Clinical Investigation 87 (1): 203–7. January 1991. doi:10.1172/JCI114972. PMID 1670635.
- "Heterozygous mutations at the mut locus in fibroblasts with mut0 methylmalonic acidemia identified by polymerase-chain-reaction cDNA cloning". American Journal of Human Genetics 47 (5): 808–14. November 1990. PMID 1977311.
- "Structure of the human methylmalonyl-CoA mutase (MUT) locus". Genomics 8 (4): 710–6. December 1990. doi:10.1016/0888-7543(90)90259-W. PMID 1980486.
- "Molecular cloning of L-methylmalonyl-CoA mutase: gene transfer and analysis of mut cell lines". Proceedings of the National Academy of Sciences of the United States of America 85 (10): 3518–21. May 1988. doi:10.1073/pnas.85.10.3518. PMID 2453061. Bibcode: 1988PNAS...85.3518L.
- "Cloning of full-length methylmalonyl-CoA mutase from a cDNA library using the polymerase chain reaction". Genomics 4 (2): 198–205. February 1989. doi:10.1016/0888-7543(89)90300-5. PMID 2567699.
- "Immunochemical studies of fibroblasts from patients with methylmalonyl-CoA mutase apoenzyme deficiency: detection of a mutation interfering with mitochondrial import". Proceedings of the National Academy of Sciences of the United States of America 84 (5): 1421–4. March 1987. doi:10.1073/pnas.84.5.1421. PMID 2881300. Bibcode: 1987PNAS...84.1421F.
- "Linkage relationships of the human methylmalonyl CoA mutase to the HLA and D6S4 loci on chromosome 6". Genomics 3 (4): 396–8. November 1988. doi:10.1016/0888-7543(88)90135-8. PMID 2907507.
- "Isolation and characterization of methylmalonyl-CoA mutase from human placenta". The Journal of Biological Chemistry 255 (7): 2708–12. April 1980. doi:10.1016/S0021-9258(19)85795-2. PMID 6102092.
- "Purification and properties of methylmalonyl coenzyme A mutase from human liver". Archives of Biochemistry and Biophysics 214 (2): 815–23. April 1982. doi:10.1016/0003-9861(82)90088-1. PMID 6124211.
- "Cloning and expression of mutations demonstrating intragenic complementation in mut0 methylmalonic aciduria". The Journal of Clinical Investigation 93 (4): 1812–9. April 1994. doi:10.1172/JCI117166. PMID 7909321.
- "Clustering of mutations in methylmalonyl CoA mutase associated with mut- methylmalonic acidemia". American Journal of Human Genetics 55 (1): 42–50. July 1994. PMID 7912889.
- "Expression and kinetic characterization of methylmalonyl-CoA mutase from patients with the mut- phenotype: evidence for naturally occurring interallelic complementation". Human Molecular Genetics 6 (9): 1457–64. September 1997. doi:10.1093/hmg/6.9.1457. PMID 9285782.
External links
- GeneReviews/NIH/NCBI/UW entry on Methylmalonic Acidemia
- Methylmalonyl-CoA+Mutase at the US National Library of Medicine Medical Subject Headings (MeSH)
![]() | Original source: https://en.wikipedia.org/wiki/Methylmalonyl-CoA mutase.
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