Biology:Photosystem I

From HandWiki
Short description: Second protein complex in photosynthetic light reactions
Light-dependent reactions of photosynthesis at the thylakoid membrane
Photosystem I
PSI+LHC.png
Plant photosystem I with LHC I
Identifiers
EC number1.97.1.12
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Location of the psa genes in the chloroplast genome of Arabidopsis thaliana. The 21 protein-coding genes involved in photosynthesis are displayed as green boxes.

Photosystem I (PSI, or plastocyanin–ferredoxin oxidoreductase) is one of two photosystems in the photosynthetic light reactions of algae, plants, and cyanobacteria. Photosystem I [1] is an integral membrane protein complex that uses light energy to catalyze the transfer of electrons across the thylakoid membrane from plastocyanin to ferredoxin. Ultimately, the electrons that are transferred by Photosystem I are used to produce the moderate-energy hydrogen carrier NADPH.[2] The photon energy absorbed by Photosystem I also produces a proton-motive force that is used to generate ATP. PSI is composed of more than 110 cofactors, significantly more than Photosystem II.[3]

History

This photosystem is known as PSI because it was discovered before Photosystem II, although future experiments showed that Photosystem II is actually the first enzyme of the photosynthetic electron transport chain. Aspects of PSI were discovered in the 1950s, but the significance of these discoveries was not yet recognized at the time.[4] Louis Duysens first proposed the concepts of Photosystems I and II in 1960, and, in the same year, a proposal by Fay Bendall and Robert Hill assembled earlier discoveries into a coherent theory of serial photosynthetic reactions.[4] Hill and Bendall's hypothesis was later confirmed in experiments conducted in 1961 by the Duysens and Witt groups.[4]

Components and action

Two main subunits of PSI, PsaA and PsaB, are closely related proteins involved in the binding of the vital electron transfer cofactors P700, Acc, A0, A1, and Fx. PsaA and PsaB are both integral membrane proteins of 730 to 750 amino acids that contain 11 transmembrane segments. A [4Fe-4S] iron-sulfur cluster called Fx is coordinated by four cysteines; two cysteines are provided each by PsaA and PsaB. The two cysteines in each are proximal and located in a loop between the ninth and tenth transmembrane segments. A leucine zipper motif seems to be present [5] downstream of the cysteines and could contribute to dimerisation of PsaA/PsaB. The terminal electron acceptors FA and FB, also [4Fe-4S] iron-sulfur clusters, are located in a 9-kDa protein called PsaC that binds to the PsaA/PsaB core near FX.[6][7]

Components of PSI (protein subunits, lipids, pigments, coenzymes, and cofactors).[8]
Protein subunits Description
PsaA Related large transmembrane proteins involved in the binding of P700, A0, A1, and Fx. Part of the photosynthetic reaction centre protein family.
PsaB
PsaC Iron-sulfur center; apoprotein for Fa and Fb
PsaD Required for assembly, helps bind ferredoxin. InterPro: IPR003685
PsaE InterPro: IPR003375
PsaI May stabilize PsaL. Stabilizes light-harvesting complex II binding.[9] InterPro: IPR001302
PsaJ InterPro: IPR002615
PsaK InterPro: IPR035982
PsaL InterPro: IPR036592
PsaM InterPro: IPR010010
PsaX InterPro: IPR012986
cytochrome b6f complex Soluble protein
Fa From PsaC; In electron transport chain (ETC)
Fb From PsaC; In ETC
Fx From PsaAB; In ETC
Ferredoxin Electron carrier in ETC
Plastocyanin Soluble protein
Lipids Description
MGDG II Monogalactosyldiglyceride lipid
PG I Phosphatidylglycerol phospholipid
PG III Phosphatidylglycerol phospholipid
PG IV Phosphatidylglycerol phospholipid
Pigments Description
Chlorophyll a 90 pigment molecules in antenna system
Chlorophyll a 5 pigment molecules in ETC
Chlorophyll a0 Early electron acceptor of modified chlorophyll in ETC
Chlorophyll a′ 1 pigment molecule in ETC
β-Carotene 22 carotenoid pigment molecules
Coenzymes and cofactors Description
QK-A Early electron acceptor vitamin K1 phylloquinone in ETC
QK-B Early electron acceptor vitamin K1 phylloquinone in ETC
FNR Ferredoxin-NADP+ oxidoreductase enzyme
Ca2+ Calcium ion
Mg2+ Magnesium ion

Photon

Photoexcitation of the pigment molecules in the antenna complex induces electron and energy transfer.[10]

Antenna complex

The antenna complex is composed of molecules of chlorophyll and carotenoids mounted on two proteins.[11] These pigment molecules transmit the resonance energy from photons when they become photoexcited. Antenna molecules can absorb all wavelengths of light within the visible spectrum.[12] The number of these pigment molecules varies from organism to organism. For instance, the cyanobacterium Synechococcus elongatus (Thermosynechococcus elongatus) has about 100 chlorophylls and 20 carotenoids, whereas spinach chloroplasts have around 200 chlorophylls and 50 carotenoids.[12][3] Located within the antenna complex of PSI are molecules of chlorophyll called P700 reaction centers. The energy passed around by antenna molecules is directed to the reaction center. There may be as many as 120 or as few as 25 chlorophyll molecules per P700.[13]

P700 reaction center

The P700 reaction center is composed of modified chlorophyll a that best absorbs light at a wavelength of 700 nm.[14] P700 receives energy from antenna molecules and uses the energy from each photon to raise an electron to a higher energy level (P700*). These electrons are moved in pairs in an oxidation/reduction process from P700* to electron acceptors, leaving behind P700+. The pair of P700* - P700+ has an electric potential of about −1.2 volts. The reaction center is made of two chlorophyll molecules and is therefore referred to as a dimer.[11] The dimer is thought to be composed of one chlorophyll a molecule and one chlorophyll a′ molecule. However, if P700 forms a complex with other antenna molecules, it can no longer be a dimer.[13]

Modified chlorophyll A0 and A1

The two modified chlorophyll molecules are early electron acceptors in PSI. They are present one per PsaA/PsaB side, forming two branches electrons can take to reach Fx. A0 accepts electrons from P700*, passes it to A1 of the same side, which then passes the electron to the quinone on the same side. Different species seems to have different preferences for either A/B branch.[15]

Phylloquinone

A phylloquinone, sometimes called vitamin K1,[16] is the next early electron acceptor in PSI. It oxidizes A1 in order to receive the electron and in turn is re-oxidized by Fx, from which the electron is passed to Fb and Fa.[16][17] The reduction of Fx appears to be the rate-limiting step.[15]

Iron–sulfur complex

Three proteinaceous iron–sulfur reaction centers are found in PSI. Labeled Fx, Fa, and Fb, they serve as electron relays.[18] Fa and Fb are bound to protein subunits of the PSI complex and Fx is tied to the PSI complex.[18] Various experiments have shown some disparity between theories of iron–sulfur cofactor orientation and operation order.[18] In one model, Fx passes an electron to Fa, which passes it on to Fb to reach the ferredoxin.[15]

Ferredoxin

Ferredoxin (Fd) is a soluble protein that facilitates reduction of NADP+ to NADPH.[19] Fd moves to carry an electron either to a lone thylakoid or to an enzyme that reduces NADP+.[19] Thylakoid membranes have one binding site for each function of Fd.[19] The main function of Fd is to carry an electron from the iron-sulfur complex to the enzyme ferredoxin–NADP+ reductase.[19]

Ferredoxin–NADP+ reductase (FNR)

This enzyme transfers the electron from reduced ferredoxin to NADP+ to complete the reduction to NADPH.[20] FNR may also accept an electron from NADPH by binding to it.[20]

Plastocyanin

Plastocyanin is an electron carrier that transfers the electron from cytochrome b6f to the P700 cofactor of PSI in its ionized state P700+.[10][21]

Ycf4 protein domain

The Ycf4 protein domain found on the thylakoid membrane is vital to photosystem I. This thylakoid transmembrane protein helps assemble the components of photosystem I. Without it, photosynthesis would be inefficient.[22]

Evolution

Molecular data show that PSI likely evolved from the photosystems of green sulfur bacteria. The photosystems of green sulfur bacteria and those of cyanobacteria, algae, and higher plants are not the same, but there are many analogous functions and similar structures. Three main features are similar between the different photosystems.[23] First, redox potential is negative enough to reduce ferredoxin.[23] Next, the electron-accepting reaction centers include iron–sulfur proteins.[23] Last, redox centres in complexes of both photosystems are constructed upon a protein subunit dimer.[23] The photosystem of green sulfur bacteria even contains all of the same cofactors of the electron transport chain in PSI.[23] The number and degree of similarities between the two photosystems strongly indicates that PSI and the analogous photosystem of green sulfur bacteria evolved from a common ancestral photosystem.

See also

References

  1. ↑ "Structure, function and organization of the Photosystem I reaction center complex". Biochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics 895 (3): 167–204. 1987. doi:10.1016/s0304-4173(87)80002-2. PMID 3333014. 
  2. ↑ "Physiological Functions of Cyclic Electron Transport Around Photosystem I in Sustaining Photosynthesis and Plant Growth". Annual Review of Plant Biology 67: 81–106. April 2016. doi:10.1146/annurev-arplant-043015-112002. PMID 26927905. 
  3. ↑ 3.0 3.1 "Structure and function of photosystems I and II". Annual Review of Plant Biology 57: 521–65. 2006. doi:10.1146/annurev.arplant.57.032905.105350. PMID 16669773. 
  4. ↑ 4.0 4.1 4.2 "Unraveling the photosystem I reaction center: a history, or the sum of many efforts". Photosynthesis Research 80 (1–3): 109–24. 2004. doi:10.1023/B:PRES.0000030657.88242.e1. PMID 16328814. 
  5. ↑ "Photosystem I reaction-centre proteins contain leucine zipper motifs. A proposed role in dimer formation". FEBS Letters 264 (1): 1–4. May 1990. doi:10.1016/0014-5793(90)80749-9. PMID 2186925. 
  6. ↑ "Breaking biological symmetry in membrane proteins: the asymmetrical orientation of PsaC on the pseudo-C2 symmetric Photosystem I core". Cellular and Molecular Life Sciences 66 (7): 1257–70. April 2009. doi:10.1007/s00018-009-8673-x. PMID 19132290. 
  7. ↑ "Understanding of the binding interface between PsaC and the PsaA/PsaB heterodimer in photosystem I". Biochemistry 48 (23): 5405–16. June 2009. doi:10.1021/bi900243f. PMID 19432395. 
  8. ↑ "The assembly of protein subunits and cofactors in photosystem I". Current Opinion in Structural Biology 12 (2): 244–54. April 2002. doi:10.1016/S0959-440X(02)00317-2. PMID 11959504. 
  9. ↑ Plöchinger, Magdalena; Torabi, Salar; Rantala, Marjaana; Tikkanen, Mikko; Suorsa, Marjaana; Jensen, Poul-Erik; Aro, Eva Mari; Meurer, Jörg (September 2016). "The Low Molecular Weight Protein PsaI Stabilizes the Light-Harvesting Complex II Docking Site of Photosystem I". Plant Physiology 172 (1): 450–463. doi:10.1104/pp.16.00647. PMID 27406169. 
  10. ↑ 10.0 10.1 Raven, Peter H.; Evert, Ray F.; Eichhorn, Susan E. (2005). "Photosynthesis, Light, and Life". Biology of Plants (7th ed.). New York: W. H. Freeman. pp. 121–127. ISBN 978-0-7167-1007-3. https://archive.org/details/biologyofplants00rave_0. 
  11. ↑ 11.0 11.1 Zeiger, Eduardo; Taiz, Lincoln (2006). "Ch. 7: Topic 7.8: Photosystem I". Plant Physiology (4th ed.). Sunderland, MA: Sinauer Associates. ISBN 0-87893-856-7. http://4e.plantphys.net/article.php?ch=3&id=73. 
  12. ↑ 12.0 12.1 "The Photosynthetic Process". http://kentsimmons.uwinnipeg.ca/cm1504/lightreact.htm. 
  13. ↑ 13.0 13.1 "Molecular arrangement of pigment-protein complex of photosystem 1". Photosynthesis Research 9 (1–2): 3–12. January 1986. doi:10.1007/BF00029726. PMID 24442279. 
  14. ↑ "Primary photochemistry in photosystem-I". Photosynthesis Research 6 (4): 295–316. December 1985. doi:10.1007/BF00054105. PMID 24442951. 
  15. ↑ 15.0 15.1 15.2 Grotjohann, I; Fromme, P (2013). "Photosystem I". Encyclopedia of biological chemistry (Second ed.). London. pp. 503–507. doi:10.1016/B978-0-12-378630-2.00287-5. ISBN 978-0-12-378630-2. 
  16. ↑ 16.0 16.1 Itoh, Shigeru; Iwaki, Masayo (1989). "Vitamin K1 (Phylloquinone) Restores the Turnover of FeS centers of Ether-extracted Spinach PSI Particles". FEBS Letters 243 (1): 47–52. doi:10.1016/0014-5793(89)81215-3. 
  17. ↑ "Is phylloquinone an obligate electron carrier in photosystem I?". FEBS Letters 215 (1): 58–62. May 1987. doi:10.1016/0014-5793(87)80113-8. PMID 3552735. 
  18. ↑ 18.0 18.1 18.2 "Iron-sulfur clusters in type I reaction centers". Biochimica et Biophysica Acta (BBA) - Bioenergetics 1507 (1–3): 139–60. October 2001. doi:10.1016/S0005-2728(01)00197-9. PMID 11687212. 
  19. ↑ 19.0 19.1 19.2 19.3 Forti, Georgio; Maria, Paola; Grubas, Giovanna (1985). "Two Sites of Interaction of Ferredoxin with thylakoids". FEBS Letters 186 (2): 149–152. doi:10.1016/0014-5793(85)80698-0. 
  20. ↑ 20.0 20.1 "Investigation of the Diaphorase Reaction of Ferredoxin–NADP+ Reductase by Electrochemical Methods". Bioelectrochemistry and Bioenergetics 47 (1): 179–183. November 1998. doi:10.1016/S0302-4598(98)00175-5. http://www.unizar.es/departamentos/bioquimica_biologia/investigacion/mmedina/Madoz1998.pdf. 
  21. ↑ "Electron transfers amongst cytochrome f, plastocyanin and photosystem I: kinetics and mechanisms". Biochimica et Biophysica Acta (BBA) - Bioenergetics 1456 (1): 5–26. January 2000. doi:10.1016/S0005-2728(99)00101-2. PMID 10611452. 
  22. ↑ "The chloroplast ycf3 and ycf4 open reading frames of Chlamydomonas reinhardtii are required for the accumulation of the photosystem I complex". The EMBO Journal 16 (20): 6095–104. October 1997. doi:10.1093/emboj/16.20.6095. PMID 9321389. 
  23. ↑ 23.0 23.1 23.2 23.3 23.4 Lockau, Wolfgang; Nitschke, Wolfgang (1993). "Photosystem I and its Bacterial Counterparts". Physiologia Plantarum 88 (2): 372–381. doi:10.1111/j.1399-3054.1993.tb05512.x. 

External links



Retrieved from "https://handwiki.org/wiki/index.php?title=Biology:Photosystem_I&oldid=3522914"