Replication crisis

Short description: Observed inability to reproduce scientific studies

Ioannidis (2005): "Why Most Published Research Findings Are False".

The replication crisis (also called the replicability crisis and the reproducibility crisis) is an ongoing methodological crisis in which the results of many scientific studies are difficult or impossible to reproduce. Because the reproducibility of empirical results is an essential part of the scientific method, such failures undermine the credibility of theories building on them and potentially call into question substantial parts of scientific knowledge.

The replication crisis is frequently discussed in relation to psychology and medicine, where considerable efforts have been undertaken to reinvestigate classic results, to determine whether they are reliable, and if they turn out not to be, the reasons for the failure. Data strongly indicates that other natural and social sciences are affected as well.

The phrase replication crisis was coined in the early 2010s as part of a growing awareness of the problem. Considerations of causes and remedies have given rise to a new scientific discipline, metascience, which uses methods of empirical research to examine empirical research practice.

Considerations about reproducibility fall into two categories. Reproducibility in the narrow sense refers to re-examining and validating the analysis of a given set of data. Replication refers to repeating the experiment or study to obtain new, independent data with the goal of reaching the same or similar conclusions.

Background

Replication has been called "the cornerstone of science". Environmental health scientist Stefan Schmidt began a 2009 review with this description of replication:

Replication is one of the central issues in any empirical science. To confirm results or hypotheses by a repetition procedure is at the basis of any scientific conception. A replication experiment to demonstrate that the same findings can be obtained in any other place by any other researcher is conceived as an operationalization of objectivity. It is the proof that the experiment reflects knowledge that can be separated from the specific circumstances (such as time, place, or persons) under which it was gained.

But there is limited consensus on how to define replication and potentially related concepts. A number of types of replication have been identified:

1. Direct or exact replication, where an experimental procedure is repeated as closely as possible.
2. Systematic replication, where an experimental procedure is largely repeated, with some intentional changes.
3. Conceptual replication, where a finding or hypothesis is tested using a different procedure. Conceptual replication allows testing for generalizability and veracity of a result or hypothesis.

Reproducibility can also be distinguished from replication, as referring to reproducing the same results using the same data set. Reproducibility of this type is why many researchers make their data available to others for testing.

The replication crisis does not necessarily mean these fields are unscientific. Rather, this process is part of the scientific process in which old ideas or those that cannot withstand careful scrutiny are pruned, although this pruning process is not always effective.

A hypothesis is generally considered to be supported when the results match the predicted pattern and that pattern of results is found to be statistically significant. Results are considered significant whenever the relative frequency of the observed pattern falls below an arbitrarily chosen value (i.e. the significance level) when assuming the null hypothesis is true. This generally answers the question of how unlikely results would be if no difference existed at the level of the statistical population. If the probability associated with the test statistic exceeds the chosen critical value, the results are considered statistically significant. The corresponding probability of exceeding the critical value is depicted as p < 0.05, where p (typically referred to as the "p-value") is the probability level. This should result in 5% of hypotheses that are supported being false positives (an incorrect hypothesis being erroneously found correct), assuming the studies meet all of the statistical assumptions. Some fields use smaller p-values, such as p < 0.01 (1% chance of a false positive) or p < 0.001 (0.1% chance of a false positive). But a smaller chance of a false positive often requires greater sample sizes or a greater chance of a false negative (a correct hypothesis being erroneously found incorrect). Although p-value testing is the most commonly used method, it is not the only method.

History

The beginning of the replication crisis can be traced to a number of events in the early 2010s. Philosopher of science and social epistemologist Felipe Romero identified four events that can be considered precursors to the ongoing crisis:

• Controversies around social priming research: In the early 2010s, the well-known "elderly-walking" study by social psychologist John Bargh and colleagues failed to replicate in two direct replications. This experiment was part of a series of three studies that had been widely cited throughout the years, was regularly taught in university courses, and had inspired a large number of conceptual replications. Failures to replicate the study led to much controversy and a heated debate involving the original authors. Notably, many of the conceptual replications of the original studies also failed to replicate in subsequent direct replications.
• Controversies around experiments on extrasensory perception: Social psychologist Daryl Bem conducted a series of experiments supposedly providing evidence for the controversial phenomenon of extrasensory perception. Bem was highly criticized for his study's methodology and upon reanalysis of the data, no evidence was found for the existence of extrasensory perception. The experiment also failed to replicate in subsequent direct replications. According to Romero, what the community found particularly upsetting was that many of the flawed procedures and statistical tools used in Bem's studies were part of common research practice in psychology.
• Amgen and Bayer reports on lack of replicability in biomedical research: Scientists from biotech companies Amgen and Bayer Healthcare reported alarmingly low replication rates (11–20%) of landmark findings in preclinical oncological research.
• Publication of studies on p-hacking and questionable research practices: Since the late 2000s, a number of studies in metascience showed how commonly adopted practices in many scientific fields, such as exploiting the flexibility of the process of data collection and reporting, could greatly increase the probability of false positive results. These studies suggested how a significant proportion of published literature in several scientific fields could be nonreplicable research.

This series of events generated a great deal of skepticism about the validity of existing research in light of widespread methodological flaws and failures to replicate findings. This led prominent scholars to declare a "crisis of confidence" in psychology and other fields, and the ensuing situation came to be known as the "replication crisis".

Although the beginning of the replication crisis can be traced to the early 2010s, some authors point out that concerns about replicability and research practices in the social sciences had been expressed much earlier. Romero notes that authors voiced concerns about the lack of direct replications in psychological research in the late 1960s and early 1970s. He also writes that certain studies in the 1990s were already reporting that journal editors and reviewers are generally biased against publishing replication studies.

In the social sciences, the blog Data Colada (whose three authors coined the term "p-hacking" in a 2014 paper) has been credited with contributing to the start of the replication crisis.

University of Virginia professor and cognitive psychologist Barbara A. Spellman has written that many criticisms of research practices and concerns about replicability of research are not new. She reports that between the late 1950s and the 1990s, scholars were already expressing concerns about a possible crisis of replication, a suspiciously high rate of positive findings, questionable research practices (QRPs), the effects of publication bias, issues with statistical power, and bad standards of reporting.

Spellman also identifies reasons that the reiteration of these criticisms and concerns in recent years led to a full-blown crisis and challenges to the status quo. First, technological improvements facilitated conducting and disseminating replication studies, and analyzing large swaths of literature for systemic problems. Second, the research community's increasing size and diversity made the work of established members more easily scrutinized by other community members unfamiliar with them. According to Spellman, these factors, coupled with increasingly limited resources and misaligned incentives for doing scientific work, led to a crisis in psychology and other fields.

Prevalence

In psychology

Several factors have combined to put psychology at the center of the conversation. Some areas of psychology once considered solid, such as social priming, have come under increased scrutiny due to failed replications. Much of the focus has been on the area of social psychology, although other areas of psychology such as clinical psychology, developmental psychology, and educational research have also been implicated.

In August 2015, the first open empirical study of reproducibility in psychology was published, called The Reproducibility Project: Psychology. Coordinated by psychologist Brian Nosek, researchers redid 100 studies in psychological science from three high-ranking psychology journals (Journal of Personality and Social Psychology, Journal of Experimental Psychology: Learning, Memory, and Cognition, and Psychological Science). 97 of the original studies had significant effects, but of those 97, only 36% of the replications yielded significant findings (p value below 0.05). The mean effect size in the replications was approximately half the magnitude of the effects reported in the original studies. The same paper examined the reproducibility rates and effect sizes by journal and discipline. Study replication rates were 23% for the Journal of Personality and Social Psychology, 48% for Journal of Experimental Psychology: Learning, Memory, and Cognition, and 38% for Psychological Science. Studies in the field of cognitive psychology had a higher replication rate (50%) than studies in the field of social psychology (25%).

A study published in 2018 in Nature Human Behaviour replicated 21 social and behavioral science papers from Nature and Science, finding that only about 62% could successfully reproduce original results.

Similarly, in a study conducted under the auspices of the Center for Open Science, a team of 186 researchers from 60 different laboratories (representing 36 different nationalities from six different continents) conducted replications of 28 classic and contemporary findings in psychology. The study's focus was not only whether the original papers' findings replicated but also the extent to which findings varied as a function of variations in samples and contexts. Overall, 50% of the 28 findings failed to replicate despite massive sample sizes. But if a finding replicated, then it replicated in most samples. If a finding was not replicated, then it failed to replicate with little variation across samples and contexts. This evidence is inconsistent with a proposed explanation that failures to replicate in psychology are likely due to changes in the sample between the original and replication study.

Results of a 2022 study suggest that many earlier brain–phenotype studies ("brain-wide association studies" (BWAS)) produced invalid conclusions as the replication of such studies requires samples from thousands of individuals due to small effect sizes.

In medicine

Results from The Reproducibility Project: Cancer Biology suggest most studies of the cancer research sector may not be replicable.

Of 49 medical studies from 1990 to 2003 with more than 1000 citations, 92% found that the studied therapies were effective. Of these studies, 16% were contradicted by subsequent studies, 16% had found stronger effects than did subsequent studies, 44% were replicated, and 24% remained largely unchallenged. A 2011 analysis by researchers with pharmaceutical company Bayer found that, at most, a quarter of Bayer's in-house findings replicated the original results. But the analysis of Bayer's results found that the results that did replicate could often be successfully used for clinical applications.

In a 2012 paper, C. Glenn Begley, a biotech consultant working at Amgen, and Lee Ellis, a medical researcher at the University of Texas, found that only 11% of 53 pre-clinical cancer studies had replications that could confirm conclusions from the original studies. In late 2021, The Reproducibility Project: Cancer Biology examined 53 top papers about cancer published between 2010 and 2012 and showed that among studies that provided sufficient information to be redone, the effect sizes were 85% smaller on average than the original findings. A survey of cancer researchers found that half of them had been unable to reproduce a published result. Another report estimated that almost half of randomized controlled trials contained flawed data (based on the analysis of anonymized individual participant data (IPD) from more than 150 trials).

In other disciplines

In economics

Economics has lagged behind other social sciences and psychology in its attempts to assess replication rates and increase the number of studies that attempt replication. A 2016 study in the journal Science replicated 18 experimental studies published in two leading economics journals, The American Economic Review and the Quarterly Journal of Economics, between 2011 and 2014. It found that about 39% failed to reproduce the original results. About 20% of studies published in The American Economic Review are contradicted by other studies despite relying on the same or similar data sets. A study of empirical findings in the Strategic Management Journal found that about 30% of 27 retested articles showed statistically insignificant results for previously significant findings, whereas about 4% showed statistically significant results for previously insignificant findings.

In water resource management

A 2019 study in Scientific Data estimated with 95% confidence that of 1,989 articles on water resources and management published in 2017, study results might be reproduced for only 0.6% to 6.8%, even if each of these articles were to provide sufficient information that allowed for replication.

Across fields

A 2016 survey by Nature on 1,576 researchers who took a brief online questionnaire on reproducibility found that more than 70% of researchers have tried and failed to reproduce another scientist's experiment results (including 87% of chemists, 77% of biologists, 69% of physicists and engineers, 67% of medical researchers, 64% of earth and environmental scientists, and 62% of all others), and more than half have failed to reproduce their own experiments. But fewer than 20% had been contacted by another researcher unable to reproduce their work. The survey found that fewer than 31% of researchers believe that failure to reproduce results means that the original result is probably wrong, although 52% agree that a significant replication crisis exists. Most researchers said they still trust the published literature. In 2010, Fanelli (2010) found that 91.5% of psychiatry/psychology studies confirmed the effects they were looking for, and concluded that the odds of this happening (a positive result) was around five times higher than in fields such as astronomy or geosciences. Fanelli argued that this is because researchers in "softer" sciences have fewer constraints to their conscious and unconscious biases.

Early analysis of result-blind peer review, which is less affected by publication bias, has estimated that 61% of result-blind studies in biomedicine and psychology have led to null results, in contrast to an estimated 5% to 20% in earlier research.

Causes

The replication crisis may be triggered by the "generation of new data and scientific publications at an unprecedented rate" that leads to the "desperation to publish or perish" and a failure to adhere to good scientific practice.

Historical and sociological roots

Predictions of an impending crisis in the quality control mechanism of science can be traced back several decades. Derek de Solla Price—considered the father of scientometrics, the quantitative study of science—predicted in 1963 that science could reach "senility" as a result of its own exponential growth. Some present-day literature seems to vindicate this "overflow" prophecy, lamenting the decay in both attention and quality.

Historian Philip Mirowski argues that the decline of scientific quality can be connected to its commodification, especially spurred by the profit-driven decision of major corporations to outsource their research to universities and contract research organizations.

Social systems theory, as expounded in the work of German sociologist Niklas Luhmann, inspires a similar diagnosis. This theory holds that each system, such as economy, science, religion or media, communicates using its own code: true and false for science, profit and loss for the economy, news and no-news for the media, and so on. According to some sociologists, science's mediatization, its commodification and its politicization, as a result of the structural coupling among systems, have led to a confusion of the original system codes.

Problems with the publication system in science

Publication bias

A major cause of low reproducibility is the publication bias stemming from the fact that statistically non-significant results and seemingly unoriginal replications are rarely published. Only a very small proportion of academic journals in psychology and neurosciences explicitly welcomed submissions of replication studies in their aim and scope or instructions to authors. This does not encourage reporting on, or even attempts to perform, replication studies. Among 1,576 researchers Nature surveyed in 2016, only a minority had ever attempted to publish a replication, and several respondents who had published failed replications noted that editors and reviewers demanded that they play down comparisons with the original studies. An analysis of 4,270 empirical studies in 18 business journals from 1970 to 1991 reported that less than 10% of accounting, economics, and finance articles and 5% of management and marketing articles were replication studies. Publication bias is augmented by the pressure to publish and the author's own confirmation bias, and is an inherent hazard in the field, requiring a certain degree of skepticism on the part of readers.

Publication bias leads to what psychologist Robert Rosenthal calls the "file drawer effect". The file drawer effect is the idea that as a consequence of the publication bias, a significant number of negative results are not published. According to philosopher of science Felipe Romero, this tends to produce "misleading literature and biased meta-analytic studies", and when publication bias is considered along with the fact that a majority of tested hypotheses might be false a priori, it is plausible that a considerable proportion of research findings might be false positives, as shown by metascientist John Ioannidis. In turn, a high proportion of false positives in the published literature can explain why many findings are nonreproducible.

"Publish or perish" culture

Further information: Publish or perish

The consequences for replicability of the publication bias are exacerbated by academia's "publish or perish" culture. As explained by metascientist Daniele Fanelli, "publish or perish" culture is a sociological aspect of academia whereby scientists work in an environment with very high pressure to have their work published in recognized journals. This is the consequence of the academic work environment being hypercompetitive and of bibliometric parameters (e.g., number of publications) being increasingly used to evaluate scientific careers. According to Fanelli, this pushes scientists to employ a number of strategies aimed at making results "publishable". In the context of publication bias, this can mean adopting behaviors aimed at making results positive or statistically significant, often at the expense of their validity (see QRPs, section 4.3).

According to Center for Open Science founder Brian Nosek and his colleagues, "publish or perish" culture created a situation whereby the goals and values of single scientists (e.g., publishability) are not aligned with the general goals of science (e.g., pursuing scientific truth). This is detrimental to the validity of published findings.

Philosopher Brian D. Earp and psychologist Jim A. C. Everett argue that, although replication is in the best interests of academics and researchers as a group, features of academic psychological culture discourage replication by individual researchers. They argue that performing replications can be time-consuming, and take away resources from projects that reflect the researcher's original thinking. They are harder to publish, largely because they are unoriginal, and even when they can be published they are unlikely to be viewed as major contributions to the field. Replications "bring less recognition and reward, including grant money, to their authors".

In his 1971 book Scientific Knowledge and Its Social Problems, philosopher and historian of science Jerome R. Ravetz predicted that science—in its progression from "little" science composed of isolated communities of researchers to "big" science or "techno-science"—would suffer major problems in its internal system of quality control. He recognized that the incentive structure for modern scientists could become dysfunctional, creating perverse incentives to publish any findings, however dubious. According to Ravetz, quality in science is maintained only when there is a community of scholars, linked by a set of shared norms and standards, who are willing and able to hold each other accountable.

Standards of reporting

Certain publishing practices also make it difficult to conduct replications and to monitor the severity of the reproducibility crisis, for articles often come with insufficient descriptions for other scholars to reproduce the study. The Reproducibility Project: Cancer Biology showed that of 193 experiments from 53 top papers about cancer published between 2010 and 2012, only 50 experiments from 23 papers have authors who provided enough information for researchers to redo the studies, sometimes with modifications. None of the 193 papers examined had its experimental protocols fully described and replicating 70% of experiments required asking for key reagents. The aforementioned study of empirical findings in the Strategic Management Journal found that 70% of 88 articles could not be replicated due to a lack of sufficient information for data or procedures. In water resources and management, most of 1,987 articles published in 2017 were not replicable because of a lack of available information shared online.

Questionable research practices and fraud

Questionable research practices (QRPs) are intentional behaviors that capitalize on the gray area of acceptable scientific behavior or exploit the researcher degrees of freedom (researcher DF), which can contribute to the irreproducibility of results by increasing the probability of false positive results. Researcher DF are seen in hypothesis formulation, design of experiments, data collection and analysis, and reporting of research. Some examples of QRPs are data dredging, selective reporting, and HARKing (hypothesising after results are known). In medicine, irreproducible studies have six features in common. These include investigators not being blinded to the experimental versus the control arms, a failure to repeat experiments, a lack of positive and negative controls, failing to report all the data, inappropriate use of statistical tests, and use of reagents that were not appropriately validated.

QRPs do not include more explicit violations of scientific integrity, such as data falsification. Fraudulent research does occur, as in the case of scientific fraud by social psychologist Diederik Stapel, cognitive psychologist Marc Hauser and social psychologist Lawrence Sanna, but it appears to be uncommon.

Prevalence

According to IU professor Ernest O’Boyle and psychologist Martin Götz, around 50% of researchers surveyed across various studies admitted engaging in HARKing. In a survey of 2,000 psychologists by behavioral scientist Leslie K. John and colleagues, around 94% of psychologists admitted having employed at least one QRP. More specifically, 63% admitted failing to report all of a study's dependent measures, 28% to report all of a study's conditions, and 46% to selectively reporting studies that produced the desired pattern of results. In addition, 56% admitted having collected more data after having inspected already collected data, and 16% to having stopped data collection because the desired result was already visible. According to biotechnology researcher J. Leslie Glick's estimate in 1992, 10% to 20% of research and development studies involved either QRPs or outright fraud. The methodology used to estimate QRPs has been contested, and more recent studies suggested lower prevalence rates on average.

A 2009 meta-analysis found that 2% of scientists across fields admitted falsifying studies at least once and 14% admitted knowing someone who did. Such misconduct was, according to one study, reported more frequently by medical researchers than by others.

Statistical issues

Low statistical power

According to Deakin University professor Tom Stanley and colleagues, one plausible reason studies fail to replicate is low statistical power. This happens for three reasons. First, a replication study with low power is unlikely to succeed since, by definition, it has a low probability to detect a true effect. Second, if the original study has low power, it will yield biased effect size estimates. When conducting a priori power analysis for the replication study, this will result in underestimation of the required sample size. Third, if the original study has low power, the post-study odds of a statistically significant finding reflecting a true effect are quite low. It is therefore likely that a replication attempt of the original study would fail.

Stanley and colleagues estimated the average statistical power of psychological literature by analyzing data from 200 meta-analyses. They found that on average, psychology studies have between 33.1% and 36.4% statistical power. These values are quite low compared to the 80% considered adequate statistical power for an experiment. Across the 200 meta-analyses, the median of studies with adequate statistical power was between 7.7% and 9.1%.

In a study published in Nature, psychologist Katherine Button and colleagues conducted a similar study with 49 meta-analyses in neuroscience, estimating a median statistical power of 21%. Meta-scientist John Ioannidis and colleagues computed an estimate of average power for empirical economic research, finding a median power of 18% based on literature drawing upon 6.700 studies. In light of these results, it is plausible that a major reason for widespread failures to replicate in several scientific fields might be very low statistical power on average.

Statistical heterogeneity

As also reported by Stanley and colleagues, a further reason studies might fail to replicate is high heterogeneity of the to-be-replicated effects. In meta-analysis, "heterogeneity" refers to the variance in research findings that results from there being no single true effect size. Instead, findings in such cases are better seen as a distribution of true effects. Statistical heterogeneity is calculated using the I-squared statistic, defined as "the proportion (or percentage) of observed variation among reported effect sizes that cannot be explained by the calculated standard errors associated with these reported effect sizes". This variation can be due to differences in experimental methods, populations, cohorts, and statistical methods between replication studies. Heterogeneity poses a challenge to studies attempting to replicate previously found effect sizes. When heterogeneity is high, subsequent replications have a high probability of finding an effect size radically different than that of the original study.

Importantly, significant levels of heterogeneity are also found in direct/exact replications of a study. Stanley and colleagues discuss this while reporting a study by quantitative behavioral scientist Richard Klein and colleagues, where the authors attempted to replicate 15 psychological effects across 36 different sites in Europe and the U.S. In the study, Klein and colleagues found significant amounts of heterogeneity in 8 out of 16 effects (I-squared = 23% to 91%). Importantly, while the replication sites intentionally differed on a variety of characteristics, such differences could account for very little heterogeneity . According to Stanley and colleagues, this suggested that heterogeneity could have been a genuine characteristic of the phenomena being investigated. For instance, phenomena might be influenced by so-called "hidden moderators" – relevant factors that were previously not understood to be important in the production of a certain effect.

In their analysis of 200 meta-analyses of psychological effects, Stanley and colleagues found a median percent of heterogeneity of I-squared = 74%. According to the authors, this level of heterogeneity can be considered "huge". It is three times larger than the random sampling variance of effect sizes measured in their study. If considered along sampling error, heterogeneity yields a standard deviation from one study to the next even larger than the median effect size of the 200 meta-analyses they investigated. The authors conclude that if replication is defined by a subsequent study finding a sufficiently similar effect size to the original, replication success is not likely even if replications have very large sample sizes. Importantly, this occurs even if replications are direct or exact since heterogeneity nonetheless remains relatively high in these cases.

Others

Within economics, the replication crisis may be also exacerbated because econometric results are fragile: using different but plausible estimation procedures or data preprocessing techniques can lead to conflicting results.

Context sensitivity

New York University professor Jay Van Bavel and colleagues argue that a further reason findings are difficult to replicate is the sensitivity to context of certain psychological effects. On this view, failures to replicate might be explained by contextual differences between the original experiment and the replication, often called "hidden moderators". Van Bavel and colleagues tested the influence of context sensitivity by reanalyzing the data of the widely cited Reproducibility Project carried out by the Open Science Collaboration. They re-coded effects according to their sensitivity to contextual factors and then tested the relationship between context sensitivity and replication success in various regression models.

Context sensitivity was found to negatively correlate with replication success, such that higher ratings of context sensitivity were associated with lower probabilities of replicating an effect. Importantly, context sensitivity significantly correlated with replication success even when adjusting for other factors considered important for reproducing results (e.g., effect size and sample size of original, statistical power of the replication, methodological similarity between original and replication). In light of the results, the authors concluded that attempting a replication in a different time, place or with a different sample can significantly alter an experiment's results. Context sensitivity thus may be a reason certain effects fail to replicate in psychology.

Base rate of hypothesis accuracy

According to philosopher Alexander Bird, a possible reason for the low rates of replicability in certain scientific fields is that a majority of tested hypotheses are false a priori. On this view, low rates of replicability could be consistent with quality science. Relatedly, the expectation that most findings should replicate would be misguided and, according to Bird, a form of base rate fallacy. Bird's argument works as follows. Assuming an ideal situation of a test of significance, whereby the probability of incorrectly rejecting the null hypothesis is 5% (i.e. Type I error) and the probability of correctly rejecting the null hypothesis is 80% (i.e. Power), in a context where a high proportion of tested hypotheses are false, it is conceivable that the number of false positives would be high compared to those of true positives. For example, in a situation where only 10% of tested hypotheses are actually true, one can calculate that as much as 36% of results will be false positives.

The claim that the falsity of most tested hypotheses can explain low rates of replicability is even more relevant when considering that the average power for statistical tests in certain fields might be much lower than 80%. For example, the proportion of false positives increases to a value between 55.2% and 57.6% when calculated with the estimates of an average power between 34.1% and 36.4% for psychology studies, as provided by Stanley and colleagues in their analysis of 200 meta-analyses in the field. A high proportion of false positives would then result in many research findings being non-replicable.

Bird notes that the claim that a majority of tested hypotheses are false a priori in certain scientific fields might be plausible given factors such as the complexity of the phenomena under investigation, the fact that theories are seldom undisputed, the "inferential distance" between theories and hypotheses, and the ease with which hypotheses can be generated. In this respect, the fields Bird takes as examples are clinical medicine, genetic and molecular epidemiology, and social psychology. This situation is radically different in fields where theories have outstanding empirical basis and hypotheses can be easily derived from theories (e.g., experimental physics).

Consequences

When effects are wrongly stated as relevant in the literature, failure to detect this by replication will lead to the canonization of such false facts.

A 2021 study found that papers in leading general interest, psychology and economics journals with findings that could not be replicated tend to be cited more over time than reproducible research papers, likely because these results are surprising or interesting. The trend is not affected by publication of failed reproductions, after which only 12% of papers that cite the original research will mention the failed replication. Further, experts are able to predict which studies will be replicable, leading the authors of the 2021 study, Marta Serra-Garcia and Uri Gneezy, to conclude that experts apply lower standards to interesting results when deciding whether to publish them.

Public awareness and perceptions

Concerns have been expressed within the scientific community that the general public may consider science less credible due to failed replications. Research supporting this concern is sparse, but a nationally representative survey in Germany showed that more than 75% of Germans have not heard of replication failures in science. The study also found that most Germans have positive perceptions of replication efforts: only 18% think that non-replicability shows that science cannot be trusted, while 65% think that replication research shows that science applies quality control, and 80% agree that errors and corrections are part of science.

Response in academia

With the replication crisis of psychology earning attention, Princeton University psychologist Susan Fiske drew controversy for speaking against critics of psychology for what she called bullying and undermining the science. She called these unidentified "adversaries" names such as "methodological terrorist" and "self-appointed data police", saying that criticism of psychology should be expressed only in private or by contacting the journals. Columbia University statistician and political scientist Andrew Gelman responded to Fiske, saying that she had found herself willing to tolerate the "dead paradigm" of faulty statistics and had refused to retract publications even when errors were pointed out. He added that her tenure as editor had been abysmal and that a number of published papers she edited were found to be based on extremely weak statistics; one of Fiske's own published papers had a major statistical error and "impossible" conclusions.

Credibility revolution

Some researchers in psychology indicate that the replication crisis is a foundation for a "credibility revolution", where changes in standards by which psychological science are evaluated may include emphasizing transparency and openness, preregistering research projects, and replicating research with higher standards for evidence to improve the strength of scientific claims. Such changes may diminish the productivity of individual researchers, but this effect could be avoided by data sharing and greater collaboration. A credibility revolution could be good for the research environment.

Remedies

Focus on the replication crisis has led to renewed efforts in psychology to retest important findings. A 2013 special edition of the journal Social Psychology focused on replication studies.

Standardization as well as (requiring) transparency of the used statistical and experimental methods have been proposed. Careful documentation of the experimental set-up is considered crucial for replicability of experiments and various variables may not be documented and standardized such as animals' diets in animal studies.

A 2016 article by John Ioannidis elaborated on "Why Most Clinical Research Is Not Useful". Ioannidis describes what he views as some of the problems and calls for reform, characterizing certain points for medical research to be useful again; one example he makes is the need for medicine to be patient-centered (e.g. in the form of the Patient-Centered Outcomes Research Institute) instead of the current practice to mainly take care of "the needs of physicians, investigators, or sponsors".

Reform in scientific publishing

Metascience

Further information: Metascience

Metascience is the use of scientific methodology to study science itself. It seeks to increase the quality of scientific research while reducing waste. It is also known as "research on research" and "the science of science", as it uses research methods to study how research is done and where improvements can be made. Metascience is concerned with all fields of research and has been called "a bird's eye view of science." In Ioannidis's words, "Science is the best thing that has happened to human beings ... but we can do it better."

Meta-research continues to be conducted to identify the roots of the crisis and to address them. Methods of addressing the crisis include pre-registration of scientific studies and clinical trials as well as the founding of organizations such as CONSORT and the EQUATOR Network that issue guidelines for methodology and reporting. Efforts continue to reform the system of academic incentives, improve the peer review process, reduce the misuse of statistics, combat bias in scientific literature, and increase the overall quality and efficiency of the scientific process.

Presentation of methodology

Some authors have argued that the insufficient communication of experimental methods is a major contributor to the reproducibility crisis and that better reporting of experimental design and statistical analyses would improve the situation. These authors tend to plead for both a broad cultural change in the scientific community of how statistics are considered and a more coercive push from scientific journals and funding bodies. But concerns have been raised about the potential for standards for transparency and replication to be misapplied to qualitative as well as quantitative studies.

Business and management journals that have introduced editorial policies on data accessibility, replication, and transparency include the Strategic Management Journal, the Journal of International Business Studies, and the Management and Organization Review.

Result-blind peer review

In response to concerns in psychology about publication bias and data dredging, more than 140 psychology journals have adopted result-blind peer review. In this approach, studies are accepted not on the basis of their findings and after the studies are completed, but before they are conducted and on the basis of the methodological rigor of their experimental designs, and the theoretical justifications for their statistical analysis techniques before data collection or analysis is done. Early analysis of this procedure has estimated that 61% of result-blind studies have led to null results, in contrast to an estimated 5% to 20% in earlier research. In addition, large-scale collaborations between researchers working in multiple labs in different countries that regularly make their data openly available for different researchers to assess have become much more common in psychology.

Pre-registration of studies

Scientific publishing has begun using pre-registration reports to address the replication crisis. The registered report format requires authors to submit a description of the study methods and analyses prior to data collection. Once the method and analysis plan is vetted through peer-review, publication of the findings is provisionally guaranteed, based on whether the authors follow the proposed protocol. One goal of registered reports is to circumvent the publication bias toward significant findings that can lead to implementation of questionable research practices. Another is to encourage publication of studies with rigorous methods.

The journal Psychological Science has encouraged the preregistration of studies and the reporting of effect sizes and confidence intervals. The editor in chief also noted that the editorial staff will be asking for replication of studies with surprising findings from examinations using small sample sizes before allowing the manuscripts to be published.

Metadata and digital tools for tracking replications

It has been suggested that "a simple way to check how often studies have been repeated, and whether or not the original findings are confirmed" is needed. Categorizations and ratings of reproducibility at the study or results level, as well as addition of links to and rating of third-party confirmations, could be conducted by the peer-reviewers, the scientific journal, or by readers in combination with novel digital platforms or tools.

Statistical reform

Requiring smaller p-values

Many publications require a p-value of p < 0.05 to claim statistical significance. The paper "Redefine statistical significance", signed by a large number of scientists and mathematicians, proposes that in "fields where the threshold for defining statistical significance for new discoveries is p < 0.05, we propose a change to p < 0.005. This simple step would immediately improve the reproducibility of scientific research in many fields." Their rationale is that "a leading cause of non-reproducibility (is that the) statistical standards of evidence for claiming new discoveries in many fields of science are simply too low. Associating 'statistically significant' findings with p < 0.05 results in a high rate of false positives even in the absence of other experimental, procedural and reporting problems."

This call was subsequently criticised by another large group, who argued that "redefining" the threshold would not fix current problems, would lead to some new ones, and that in the end, all thresholds needed to be justified case-by-case instead of following general conventions.

Addressing misinterpretation of p-values

Further information: Misuse of p-values

Although statisticians are unanimous that the use of "p < 0.05" as a standard for significance provides weaker evidence than is generally appreciated, there is a lack of unanimity about what should be done about it. Some have advocated that Bayesian methods should replace p-values. This has not happened on a wide scale, partly because it is complicated and partly because many users distrust the specification of prior distributions in the absence of hard data. A simplified version of the Bayesian argument, based on testing a point null hypothesis was suggested by pharmacologist David Colquhoun. The logical problems of inductive inference were discussed in "The Problem with p-values" (2016).

The hazards of reliance on p-values arises partly because even an observation of p = 0.001 is not necessarily strong evidence against the null hypothesis. Despite the fact that the likelihood ratio in favor of the alternative hypothesis over the null is close to 100, if the hypothesis was implausible, with a prior probability of a real effect being 0.1, even the observation of p = 0.001 would have a false positive risk of 8 percent. It would still fail to reach the 5 percent level.

It was recommended that the terms "significant" and "non-significant" should not be used. p-values and confidence intervals should still be specified, but they should be accompanied by an indication of the false-positive risk. It was suggested that the best way to do this is to calculate the prior probability that would be necessary to believe in order to achieve a false positive risk of a certain level, such as 5%. The calculations can be done with various computer software. This reverse Bayesian approach, which physicist Robert Matthews suggested in 2001, is one way to avoid the problem that the prior probability is rarely known.

Encouraging larger sample sizes

To improve the quality of replications, larger sample sizes than those used in the original study are often needed. Larger sample sizes are needed because estimates of effect sizes in published work are often exaggerated due to publication bias and large sampling variability associated with small sample sizes in an original study. Further, using significance thresholds usually leads to inflated effects, because particularly with small sample sizes, only the largest effects will become significant.

Replication efforts

Funding

In July 2016, the Netherlands Organisation for Scientific Research made €3 million available for replication studies. The funding is for replication based on reanalysis of existing data and replication by collecting and analysing new data. Funding is available in the areas of social sciences, health research and healthcare innovation.

In 2013, the Laura and John Arnold Foundation funded the launch of The Center for Open Science with a $5.25 million grant. By 2017, it provided an additional $10 million in funding. It also funded the launch of the Meta-Research Innovation Center at Stanford at Stanford University run by Ioannidis and medical scientist Steven Goodman to study ways to improve scientific research. It also provided funding for the AllTrials initiative led in part by medical scientist Ben Goldacre.

Emphasis in post-secondary education

Based on coursework in experimental methods at MIT, Stanford, and the University of Washington, it has been suggested that methods courses in psychology and other fields should emphasize replication attempts rather than original studies. Such an approach would help students learn scientific methodology and provide numerous independent replications of meaningful scientific findings that would test the replicability of scientific findings. Some have recommended that graduate students should be required to publish a high-quality replication attempt on a topic related to their doctoral research prior to graduation.

Final year thesis

Some institutions require undergraduate students to submit a final year thesis that consists of an original piece of research. Daniel Quintana, a psychologist at the University of Oslo in Norway, has recommended that students should be encouraged to perform replication studies in thesis projects, as well as being taught about open science.

Semi-automated

"The overall process of testing the reproducibility and robustness of the cancer biology literature by robot. First, text mining is used to extract statements about the effect of drugs on gene expression in breast cancer. Then two different teams semi-automatically tested these statements using two different protocols, and two different cell lines (MCF7 and MDA-MB-231) using the laboratory automation system Eve."

Researchers demonstrated a way of semi-automated testing for reproducibility: statements about experimental results were extracted from, as of 2022 non-semantic, gene expression cancer research papers and subsequently reproduced via robot scientist "Eve". Problems of this approach include that it may not be feasible for many areas of research and that sufficient experimental data may not get extracted from some or many papers even if available.

Involving original authors

Psychologist Daniel Kahneman argued that, in psychology, the original authors should be involved in the replication effort because the published methods are often too vague. Others, such as psychologist Andrew Wilson, disagree, arguing that the original authors should write down the methods in detail. An investigation of replication rates in psychology in 2012 indicated higher success rates of replication in replication studies when there was author overlap with the original authors of a study (91.7% successful replication rates in studies with author overlap compared to 64.6% successful replication rates without author overlap).

Big team science

The replication crisis has led to the formation and development of various large-scale and collaborative communities to pool their resources to address a single question across cultures, countries and disciplines. The focus is on replication, to ensure that the effect generalizes beyond a specific culture and investigate whether the effect is replicable and genuine. This allows interdisciplinary internal reviews, multiple perspectives, uniform protocols across labs, and recruiting larger and more diverse samples. Researchers can collaborate by coordinating data collection or fund data collection by researchers who may not have access to the funds, allowing larger sample sizes and increasing the robustness of the conclusions.

Broader changes to scientific approach

Emphasize triangulation, not just replication

Psychologist Marcus R. Munafò and Epidemiologist George Davey Smith argue, in a piece published by Nature, that research should emphasize triangulation, not just replication, to protect against flawed ideas. They claim that,

replication alone will get us only so far (and) might actually make matters worse ... [Triangulation] is the strategic use of multiple approaches to address one question. Each approach has its own unrelated assumptions, strengths and weaknesses. Results that agree across different methodologies are less likely to be artefacts. ... Maybe one reason replication has captured so much interest is the often-repeated idea that falsification is at the heart of the scientific enterprise. This idea was popularized by Karl Popper's 1950s maxim that theories can never be proved, only falsified. Yet an overemphasis on repeating experiments could provide an unfounded sense of certainty about findings that rely on a single approach. ... philosophers of science have moved on since Popper. Better descriptions of how scientists actually work include what epistemologist Peter Lipton called in 1991 "inference to the best explanation".

Complex systems paradigm

Further information: Complex system

The dominant scientific and statistical model of causation is the linear model. The linear model assumes that mental variables are stable properties which are independent of each other. In other words, these variables are not expected to influence each other. Instead, the model assumes that the variables will have an independent, linear effect on observable outcomes.

Social scientists Sebastian Wallot and Damian Kelty-Stephen argue that the linear model is not always appropriate. An alternative is the complex system model which assumes that mental variables are interdependent. These variables are not assumed to be stable, rather they will interact and adapt to each specific context. They argue that the complex system model is often more appropriate in psychology, and that the use of the linear model when the complex system model is more appropriate will result in failed replications.

...psychology may be hoping for replications in the very measurements and under the very conditions where a growing body of psychological evidence explicitly discourages predicting replication. Failures to replicate may be plainly baked into the potentially incomplete, but broadly sweeping failure of human behavior to conform to the standard of independen[ce] ...

Replication should seek to revise theories

Replication is fundamental for scientific progress to confirm original findings. However, replication alone is not sufficient to resolve the replication crisis. Replication efforts should seek not just to support or question the original findings, but also to replace them with revised, stronger theories with greater explanatory power. This approach therefore involves pruning existing theories, comparing all the alternative theories, and making replication efforts more generative and engaged in theory-building. However, replication alone is not enough, it is important to assess the extent that results generalise across geographical, historical and social contexts is important for several scientific fields, especially practitioners and policy makers to make analyses in order to guide important strategic decisions. Reproducible and replicable findings was the best predictor of generalisability beyond historical and geographical contexts, indicating that for social sciences, results from a certain time period and place can meaningfully drive as to what is universally present in individuals.

Open science

Further information: Open science

Tenets of open science

Open data, open source software and open source hardware all are critical to enabling reproducibility in the sense of validation of the original data analysis. The use of proprietary software, the lack of the publication of analysis software and the lack of open data prevents the replication of studies. Unless software used in research is open source, reproducing results with different software and hardware configurations is impossible. CERN has both Open Data and CERN Analysis Preservation projects for storing data, all relevant information, and all software and tools needed to preserve an analysis at the large experiments of the LHC. Aside from all software and data, preserved analysis assets include metadata that enable understanding of the analysis workflow, related software, systematic uncertainties, statistics procedures and meaningful ways to search for the analysis, as well as references to publications and to backup material. CERN software is open source and available for use outside of particle physics and there is some guidance provided to other fields on the broad approaches and strategies used for open science in contemporary particle physics.

Online repositories where data, protocols, and findings can be stored and evaluated by the public seek to improve the integrity and reproducibility of research. Examples of such repositories include the Open Science Framework, Registry of Research Data Repositories, and Psychfiledrawer.org. Sites like Open Science Framework offer badges for using open science practices in an effort to incentivize scientists. However, there have been concerns that those who are most likely to provide their data and code for analyses are the researchers that are likely the most sophisticated. Ioannidis suggested that "the paradox may arise that the most meticulous and sophisticated and method-savvy and careful researchers may become more susceptible to criticism and reputation attacks by reanalyzers who hunt for errors, no matter how negligible these errors are".

See also

• Decline effect
• Estimation statistics
• Exploratory data analysis
• p-value
• Student's t-test
• Analysis of variance
• Misuse of statistics
• Data dredging
• Invalid science
• Falsifiability
• Extension neglect
• Base rate fallacy
• Selection bias
• Sampling bias
• Observer bias
• Naturalism
• Problem of induction
• Correlation does not imply causation
• Black swan theory
• Uniformitarianism

Further reading

• "Reproducibility Crisis Timeline: Milestones in Tackling Research Reliability". 5 December 2016. https://blogs.plos.org/absolutely-maybe/2016/12/05/reproducibility-crisis-timeline-milestones-in-tackling-research-reliability/. 
• Bonett, D.G. (2021). Design and analysis of replication studies. Organizational Research Methods, 24, 513–529. https://doi.org/10.1177/1094428120911088
• "A Significant Problem: Standard scientific methods are under fire. Will anything change?". Scientific American 321 (4): 62–67. October 2019. http://verso.mat.uam.es/~pablo.fernandez/Fisherb.pdf. "The use of p values for nearly a century [since 1925] to determine statistical significance of experimental results has contributed to an illusion of certainty and [to] reproducibility crises in many scientific fields. There is growing determination to reform statistical analysis... Some [researchers] suggest changing statistical methods, whereas others would do away with a threshold for defining 'significant' results.". 
• Rigor Mortis: How Sloppy Science Creates Worthless Cures, Crushes Hope, and Wastes Billions. New York: Basic Books. 2017. ISBN 9780465097906. 
• "Reproducibility and replicability of rodent phenotyping in preclinical studies". Neuroscience and Biobehavioral Reviews 87: 218–232. April 2018. doi:10.1016/j.neubiorev.2018.01.003. PMID 29357292. 
• Science Fictions: How Fraud, Bias, Negligence, and Hype Undermine the Search for Truth. New York: Metropolitan Books. July 2020. ISBN 9781250222695.  Book Review (November 2020, The American Conservative)
• "Replication Crisis". London Review of Books 43 (19): 39–40. October 2021. https://www.lrb.co.uk/the-paper/v43/n19/john-whitfield/replication-crisis.  review of Science Fictions: Exposing Fraud, Negligence and Hype in Science. London: Bodley Head. July 2020. ISBN 978-1-84792-565-7.