Biology:Glycoside hydrolase family 15
| Glycosyl hydrolases family 15 | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| Symbol | Glyco_hydro_15 | ||||||||
| Pfam | PF00723 | ||||||||
| Pfam clan | CL0059 | ||||||||
| SCOP2 | 1glm / SCOPe / SUPFAM | ||||||||
| CAZy | GH15 | ||||||||
| |||||||||
In molecular biology, glycoside hydrolase family 15 is a family of glycoside hydrolases.
Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.[1][2][3] This classification is available on the CAZy web site,[4][5] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.[6][7] y[ _]9
Glycoside hydrolase family 15 CAZY GH_15 comprises enzymes with several known activities; glucoamylase (EC 3.2.1.3); alpha-glucosidase (EC 3.2.1.20); glucodextranase (EC 3.2.1.70).
Glucoamylase (GA) catalyses the release of D-glucose from the non-reducing ends of starch and other oligo- or poly-saccharides. Studies of fungal GA have indicated 3 closely clustered acidic residues that play a role in the catalytic mechanism.[8] This region is also conserved in a recently sequenced bacterial GA.[9]
The 3D structure of the pseudo-tetrasaccharide acarbose complexed with glucoamylase II(471) from Aspergillus awamori var. X100 has been determined to 2.4A resolution.[10] The protein belongs to the mainly alpha class, and contains 19 helices and 9 strands.
References
- ↑ "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proceedings of the National Academy of Sciences of the United States of America 92 (15): 7090–4. July 1995. doi:10.1073/pnas.92.15.7090. PMID 7624375. Bibcode: 1995PNAS...92.7090H.
- ↑ "Structures and mechanisms of glycosyl hydrolases". Structure 3 (9): 853–9. September 1995. doi:10.1016/S0969-2126(01)00220-9. PMID 8535779.
- ↑ "Updating the sequence-based classification of glycosyl hydrolases". The Biochemical Journal 316 ( Pt 2) (Pt 2): 695–6. June 1996. doi:10.1042/bj3160695. PMID 8687420.
- ↑ "Home" (in en). http://www.cazy.org/.
- ↑ "The carbohydrate-active enzymes database (CAZy) in 2013". Nucleic Acids Research 42 (Database issue): D490-5. January 2014. doi:10.1093/nar/gkt1178. PMID 24270786.
- ↑ "Glycoside Hydrolase Family 15" (in en). http://www.cazypedia.org/index.php/Glycoside_Hydrolase_Family_15.
- ↑ CAZypedia Consortium (December 2018). "Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes". Glycobiology 28 (1): 3–8. doi:10.1093/glycob/cwx089. PMID 29040563. https://hal.archives-ouvertes.fr/hal-01886461/file/Hehemann_2018_01.pdf.
- ↑ "Catalytic mechanism of fungal glucoamylase as defined by mutagenesis of Asp176, Glu179 and Glu180 in the enzyme from Aspergillus awamori". Protein Engineering 3 (3): 193–8. January 1990. doi:10.1093/protein/3.3.193. PMID 1970434.
- ↑ "Molecular cloning of a glucoamylase gene from a thermophilic Clostridium and kinetics of the cloned enzyme". European Journal of Biochemistry 207 (2): 413–8. July 1992. doi:10.1111/j.1432-1033.1992.tb17064.x. PMID 1633799.
- ↑ "Refined structure for the complex of acarbose with glucoamylase from Aspergillus awamori var. X100 to 2.4-A resolution". The Journal of Biological Chemistry 269 (22): 15631–9. June 1994. doi:10.1016/S0021-9258(17)40728-9. PMID 8195212.
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