Biology:Glycoside hydrolase family 32
| Glycosyl hydrolases family 32 N-terminal domain | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 beta-fructosidase from thermotoga maritima in complex with raffinose | |||||||||
| Identifiers | |||||||||
| Symbol | Glyco_hydro_32N | ||||||||
| Pfam | PF00251 | ||||||||
| Pfam clan | CL0143 | ||||||||
| InterPro | IPR013148 | ||||||||
| PROSITE | PDOC00532 | ||||||||
| CAZy | GH32 | ||||||||
| |||||||||
| Glycosyl hydrolases family 32 C terminal | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 crystal structure of exo-inulinase from aspergillus awamori in spacegroup p21 | |||||||||
| Identifiers | |||||||||
| Symbol | Glyco_hydro_32C | ||||||||
| Pfam | PF08244 | ||||||||
| InterPro | IPR013189 | ||||||||
| PROSITE | PDOC00532 | ||||||||
| CAZy | GH32 | ||||||||
| |||||||||
In molecular biology, glycoside hydrolase family 32 is a family of glycoside hydrolases EC 3.2.1., which are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.[1][2][3] This classification is available on the CAZy web site,[4][5] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.[6][7]
Family 32 glycosyl hydrolases comprise two distinct domains. The N-terminal domain, which forms a five bladed beta propeller, and the C-terminal domain, which forms a beta sandwich structure.[8]
References
- ↑ "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proceedings of the National Academy of Sciences of the United States of America 92 (15): 7090–4. July 1995. doi:10.1073/pnas.92.15.7090. PMID 7624375. Bibcode: 1995PNAS...92.7090H.
- ↑ "Structures and mechanisms of glycosyl hydrolases". Structure 3 (9): 853–9. September 1995. doi:10.1016/S0969-2126(01)00220-9. PMID 8535779.
- ↑ "Updating the sequence-based classification of glycosyl hydrolases". The Biochemical Journal 316 (Pt 2): 695–6. June 1996. doi:10.1042/bj3160695. PMID 8687420.
- ↑ "Home". http://www.cazy.org/.
- ↑ "The carbohydrate-active enzymes database (CAZy) in 2013". Nucleic Acids Research 42 (Database issue): D490-5. January 2014. doi:10.1093/nar/gkt1178. PMID 24270786.
- ↑ "Glycoside Hydrolase Family 32" (in en). http://www.cazypedia.org/index.php/Glycoside_Hydrolase_Family_32.
- ↑ CAZypedia Consortium (December 2018). "Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes". Glycobiology 28 (1): 3–8. doi:10.1093/glycob/cwx089. PMID 29040563.
- ↑ "The three-dimensional structure of invertase (beta-fructosidase) from Thermotoga maritima reveals a bimodular arrangement and an evolutionary relationship between retaining and inverting glycosidases". The Journal of Biological Chemistry 279 (18): 18903–10. April 2004. doi:10.1074/jbc.M313911200. PMID 14973124. https://hal.archives-ouvertes.fr/hal-01818938/file/alberto_etal_2004.pdf.
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