Biology:Glycoside hydrolase family 45
| Glycosyl hydrolase family 45 | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 endoglucanase from humicola insolens at 1.7a resolution | |||||||||
| Identifiers | |||||||||
| Symbol | Glyco_hydro_45 | ||||||||
| Pfam | PF02015 | ||||||||
| Pfam clan | CL0199 | ||||||||
| InterPro | IPR000334 | ||||||||
| PROSITE | PDOC00877 | ||||||||
| SCOP2 | 2eng / SCOPe / SUPFAM | ||||||||
| CAZy | GH45 | ||||||||
| |||||||||
In molecular biology, glycoside hydrolase family 45 is a family of glycoside hydrolases.
Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.[1][2][3] This classification is available on the CAZy web site,[4][5] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.[6][7]
Glycoside hydrolase family 45 CAZY GH_45 comprises enzymes with only one known activity; endoglucanase (EC 3.2.1.4). This family is also known as cellulase family K.[8] The best conserved region in these enzymes is located in the N-terminal section. It contains an aspartic acid residue which has been shown[9] to act as a nucleophile in the catalytic mechanism. This also has several cysteines that are involved in forming disulphide bridges.
References
- ↑ "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proceedings of the National Academy of Sciences of the United States of America 92 (15): 7090–4. July 1995. doi:10.1073/pnas.92.15.7090. PMID 7624375. Bibcode: 1995PNAS...92.7090H.
- ↑ "Structures and mechanisms of glycosyl hydrolases". Structure 3 (9): 853–9. September 1995. doi:10.1016/S0969-2126(01)00220-9. PMID 8535779.
- ↑ "Updating the sequence-based classification of glycosyl hydrolases". The Biochemical Journal 316 ( Pt 2) (Pt 2): 695–6. June 1996. doi:10.1042/bj3160695. PMID 8687420.
- ↑ "Home" (in en). http://www.cazy.org/.
- ↑ "The carbohydrate-active enzymes database (CAZy) in 2013". Nucleic Acids Research 42 (Database issue): D490-5. January 2014. doi:10.1093/nar/gkt1178. PMID 24270786.
- ↑ "Glycoside Hydrolase Family 45" (in en). http://www.cazypedia.org/index.php/Glycoside_Hydrolase_Family_45.
- ↑ CAZypedia Consortium (December 2018). "Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes". Glycobiology 28 (1): 3–8. doi:10.1093/glycob/cwx089. PMID 29040563. https://hal.archives-ouvertes.fr/hal-01886461/file/Hehemann_2018_01.pdf.
- ↑ "New families in the classification of glycosyl hydrolases based on amino acid sequence similarities". The Biochemical Journal 293 ( Pt 3) (3): 781–8. August 1993. doi:10.1042/bj2930781. PMID 8352747. PMC 1134435. https://archive-ouverte.unige.ch/unige:36460/ATTACHMENT01.
- ↑ "Structure and function of endoglucanase V". Nature 365 (6444): 362–4. September 1993. doi:10.1038/365362a0. PMID 8377830. Bibcode: 1993Natur.365..362D.
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