Biology:Lacto-N-biosidase
From HandWiki
| Lacto-N-biosidase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 3.2.1.140 | ||||||||
| CAS number | 146359-52-6 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
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Lacto-N-biosidase (EC 3.2.1.140) is an enzyme with systematic name oligosaccharide lacto-N-biosylhydrolase.[1][2] This enzyme catalyses the following chemical reaction
- (1) beta-D-Gal-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-D-Glc + H2O [math]\displaystyle{ \rightleftharpoons }[/math] beta-D-Gal-(1->3)-D-GlcNAc + beta-D-Gal-(1->4)-D-Glc
- (2) lacto-N-tetraose + H2O [math]\displaystyle{ \rightleftharpoons }[/math] lacto-N-biose + lactose
The enzyme from Streptomyces specifically hydrolyses the terminal lacto-N-biosyl residue (beta-D-Gal-(1->3)-D-GlcNAc) from the non-reducing end of oligosaccharides with the structure beta-D-Gal-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->R).
References
- ↑ "An enzyme releasing lacto-N-biose from oligosaccharides". Proceedings of the National Academy of Sciences of the United States of America 89 (18): 8512–8516. September 1992. doi:10.1073/pnas.89.18.8512. PMID 1528855.
- ↑ "Purification and characterization of an enzyme releasing lacto-N-biose from oligosaccharides with type 1 chain". The Journal of Biological Chemistry 268 (25): 18560–18566. September 1993. doi:10.1016/S0021-9258(17)46664-6. PMID 7689556.
External links
- Lacto-N-biosidase at the US National Library of Medicine Medical Subject Headings (MeSH)
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