Biology:mRNA guanylyltransferase
From HandWiki
Short description: Class of enzymes
| mRNA guanylyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 2.7.7.50 | ||||||||
| CAS number | 56941-23-2 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
In enzymology, a mRNA guanylyltransferase (EC 2.7.7.50) is an enzyme that catalyzes the chemical reaction
- GTP + (5')ppPur-mRNA [math]\displaystyle{ \rightleftharpoons }[/math] diphosphate + G(5')pppPur-mRNA
Thus, the two substrates of this enzyme are GTP and (5')ppPur-mRNA, whereas its two products are diphosphate and G(5')pppPur-mRNA.
This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing nucleotide groups (nucleotidyltransferases).op The systematic name of this enzyme class is GTP:mRNA guanylyltransferase. Other names in common use include mRNA capping enzyme, messenger RNA guanylyltransferase, and Protein 2.
Structural studies
As of late 2007, 5 structures have been solved for this class of enzymes, with PDB accession codes 1CKM, 1CKN, 1CKO, 1P16, and 2C46.
References
- "Modification of the 5'-terminus of mRNA by soluble guanylyl and methyl transferases from vaccinia virus". Proc. Natl. Acad. Sci. U.S.A. 72 (7): 2525–9. 1975. doi:10.1073/pnas.72.7.2525. PMID 1058472. Bibcode: 1975PNAS...72.2525E.
- "Methylation and capping of RNA polymerase II primary transcripts by HeLa nuclear homogenates". Biochemistry 17 (6): 977–82. 1978. doi:10.1021/bi00599a005. PMID 629955.
- "Messenger RNA guanylyltransferase from Saccharomyces cerevisiae Large scale purification, subunit functions, and subcellular localization". J. Biol. Chem. 262 (5): 1989–95. 1987. doi:10.1016/S0021-9258(18)61609-6. PMID 3029058.
- "Modification of RNA by mRNA guanylyltransferase and mRNA (guanine-7-)methyltransferase from vaccinia virions". J. Biol. Chem. 250 (24): 9330–5. 1975. doi:10.1016/S0021-9258(19)40647-9. PMID 1194287.
- "Purification of mRNA guanylyltransferase and mRNA (guanine-7-) methyltransferase from vaccinia virions". J. Biol. Chem. 250 (24): 9322–9. 1975. doi:10.1016/S0021-9258(19)40646-7. PMID 1194286.
 |  |
