Biology:Poly(A)-specific ribonuclease
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Short description: Protein-coding gene in the species Homo sapiens
 Generic protein structure example |
Poly(A)-specific ribonuclease (PARN), also known as polyadenylate-specific ribonuclease or deadenylating nuclease (DAN), is an enzyme that in humans is encoded by the PARN gene.[1][2]
Function
Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. The amino acid sequence of poly(A)-specific ribonuclease shows homology to the RNase D family of 3'-exonucleases. The protein appears to be localized in both the nucleus and the cytoplasm. It is not stably associated with polysomes or ribosomal subunits.[2] Hereditary mutations in PARN lead to the bone marrow failure disease dyskeratosis congenita which is caused by defective telomerase RNA processing and degradation in patients.[3][4][5][6][7][8][9]
References
- ↑ "The human gene for the poly(A)-specific ribonuclease (PARN) maps to 16p13 and has a truncated copy in the Prader-Willi/Angelman region on 15q11→q13". Cytogenetics and Cell Genetics 87 (1–2): 125–31. May 2000. doi:10.1159/000015378. PMID 10640832.
- ↑ 2.0 2.1 "Entrez Gene: PARN poly(A)-specific ribonuclease (deadenylation nuclease)". https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=5073.
- ↑ "Poly(A)-specific ribonuclease deficiency impacts telomere biology and causes dyskeratosis congenita". The Journal of Clinical Investigation 125 (5): 2151–60. May 2015. doi:10.1172/JCI78963. PMID 25893599.
- ↑ "Bone marrow failure and developmental delay caused by mutations in poly(A)-specific ribonuclease (PARN)". Journal of Medical Genetics 52 (11): 738–48. November 2015. doi:10.1136/jmedgenet-2015-103292. PMID 26342108.
- ↑ "Exome sequencing links mutations in PARN and RTEL1 with familial pulmonary fibrosis and telomere shortening". Nature Genetics 47 (5): 512–7. May 2015. doi:10.1038/ng.3278. PMID 25848748.
- ↑ "Inhibition of telomerase RNA decay rescues telomerase deficiency caused by dyskerin or PARN defects". Nature Structural & Molecular Biology 23 (4): 286–92. April 2016. doi:10.1038/nsmb.3184. PMID 26950371.
- ↑ "Human Telomerase RNA Processing and Quality Control". Cell Reports 13 (10): 2232–43. December 2015. doi:10.1016/j.celrep.2015.10.075. PMID 26628367.
- ↑ "A Polyadenylation-Dependent 3' End Maturation Pathway Is Required for the Synthesis of the Human Telomerase RNA". Cell Reports 13 (10): 2244–57. December 2015. doi:10.1016/j.celrep.2015.11.003. PMID 26628368.
- ↑ "Poly(A)-specific ribonuclease (PARN) mediates 3'-end maturation of the telomerase RNA component". Nature Genetics 47 (12): 1482–8. December 2015. doi:10.1038/ng.3423. PMID 26482878.
Further reading
- "Poly(A) tail shortening by a mammalian poly(A)-specific 3'-exoribonuclease". The Journal of Biological Chemistry 272 (16): 10448–56. April 1997. doi:10.1074/jbc.272.16.10448. PMID 9099687.
- "The deadenylating nuclease (DAN) is involved in poly(A) tail removal during the meiotic maturation of Xenopus oocytes". The EMBO Journal 17 (18): 5427–37. September 1998. doi:10.1093/emboj/17.18.5427. PMID 9736620.
- "Cap-dependent deadenylation of mRNA". The EMBO Journal 19 (5): 1079–86. March 2000. doi:10.1093/emboj/19.5.1079. PMID 10698948.
- "A 54-kDa fragment of the Poly(A)-specific ribonuclease is an oligomeric, processive, and cap-interacting Poly(A)-specific 3' exonuclease". The Journal of Biological Chemistry 275 (31): 24222–30. August 2000. doi:10.1074/jbc.M001705200. PMID 10801819.
- "Interaction between a poly(A)-specific ribonuclease and the 5' cap influences mRNA deadenylation rates in vitro". Molecular Cell 5 (3): 479–88. March 2000. doi:10.1016/S1097-2765(00)80442-6. PMID 10882133.
- "A novel mRNA-decapping activity in HeLa cytoplasmic extracts is regulated by AU-rich elements". The EMBO Journal 20 (5): 1134–43. March 2001. doi:10.1093/emboj/20.5.1134. PMID 11230136.
- "The mRNA cap structure stimulates rate of poly(A) removal and amplifies processivity of degradation". The Journal of Biological Chemistry 276 (30): 27923–9. July 2001. doi:10.1074/jbc.M102270200. PMID 11359775.
- "The mechanism and regulation of deadenylation: identification and characterization of Xenopus PARN". RNA 7 (6): 875–86. June 2001. doi:10.1017/S1355838201010020. PMID 11424938.
- "AU binding proteins recruit the exosome to degrade ARE-containing mRNAs". Cell 107 (4): 451–64. November 2001. doi:10.1016/S0092-8674(01)00578-5. PMID 11719186.
- "Identification of the active site of poly(A)-specific ribonuclease by site-directed mutagenesis and Fe(2+)-mediated cleavage". The Journal of Biological Chemistry 277 (8): 5982–7. February 2002. doi:10.1074/jbc.M111515200. PMID 11742007.
- "Directed proteomic analysis of the human nucleolus". Current Biology 12 (1): 1–11. January 2002. doi:10.1016/S0960-9822(01)00650-9. PMID 11790298.
- "Functional proteomic analysis of human nucleolus". Molecular Biology of the Cell 13 (11): 4100–9. November 2002. doi:10.1091/mbc.E02-05-0271. PMID 12429849.
- "Tristetraprolin and its family members can promote the cell-free deadenylation of AU-rich element-containing mRNAs by poly(A) ribonuclease". Molecular and Cellular Biology 23 (11): 3798–812. June 2003. doi:10.1128/MCB.23.11.3798-3812.2003. PMID 12748283.
- "Nonsense-mediated mRNA decay in mammalian cells involves decapping, deadenylating, and exonucleolytic activities". Molecular Cell 12 (3): 675–87. September 2003. doi:10.1016/S1097-2765(03)00349-6. PMID 14527413.
- "A KH domain RNA binding protein, KSRP, promotes ARE-directed mRNA turnover by recruiting the degradation machinery". Molecular Cell 14 (5): 571–83. June 2004. doi:10.1016/j.molcel.2004.05.002. PMID 15175153.
- "A protein interaction framework for human mRNA degradation". Genome Research 14 (7): 1315–23. July 2004. doi:10.1101/gr.2122004. PMID 15231747.
- "Coordination of divalent metal ions in the active site of poly(A)-specific ribonuclease". The Journal of Biological Chemistry 279 (47): 48702–6. November 2004. doi:10.1074/jbc.M403858200. PMID 15358788.
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