Biology:RNA-induced silencing complex

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Short description: Multiprotein complex

The RNA-induced silencing complex, or RISC, is a multiprotein complex, specifically a ribonucleoprotein, which functions in gene silencing via a variety of pathways at the transcriptional and translational levels.[1] Using single-stranded RNA (ssRNA) fragments, such as microRNA (miRNA), or double-stranded small interfering RNA (siRNA), the complex functions as a key tool in gene regulation.[2] The single strand of RNA acts as a template for RISC to recognize complementary messenger RNA (mRNA) transcript. Once found, one of the proteins in RISC, Argonaute, activates and cleaves the mRNA. This process is called RNA interference (RNAi) and it is found in many eukaryotes; it is a key process in defense against viral infections, as it is triggered by the presence of double-stranded RNA (dsRNA).[3][4][1]

Discovery

The biochemical identification of RISC was conducted by Gregory Hannon and his colleagues at the Cold Spring Harbor Laboratory.[5] This was only a couple of years after the discovery of RNA interference in 1998 by Andrew Fire and Craig Mello, who shared the 2006 Nobel Prize in Physiology or Medicine.[3]

Drosophila melanogaster

Hannon and his colleagues attempted to identify the RNAi mechanisms involved in gene silencing, by dsRNAs, in Drosophila cells. Drosophila S2 cells were transfected with a lacZ expression vector to quantify gene expression with β-galactosidase activity. Their results showed co-transfection with lacZ dsRNA significantly reduced β-galactosidase activity compared to control dsRNA. Therefore, dsRNAs control gene expression via sequence complementarity.

S2 cells were then transfected with Drosophila cyclin E dsRNA. Cycline E is an essential gene for cell cycle progression into the S phase. Cyclin E dsRNA arrested the cell cycle at the G1 phase (before the S phase). Therefore, RNAi can target endogenous genes.

In addition, cyclin E dsRNA only diminished cyclin E RNA — a similar result was also shown using dsRNA corresponding to cyclin A which acts in S, G2 and M phases of the cell cycle. This shows the characteristic hallmark of RNAi: the reduced levels of mRNAs correspond to the levels of dsRNA added.

To test whether their observation of decreased mRNA levels was a result of mRNA being targeted directly (as suggested by data from other systems), Drosophila S2 cells were transfected with either Drosophila cyclin E dsRNAs or lacZ dsRNAs and then incubated with synthetic mRNAs for cyclin E or lacZ.

Cells transfected with cyclin E dsRNAs only showed degradation in cyclin E transcripts — the lacZ transcripts were stable. Conversely, cells transfected with lacZ dsRNAs only showed degradation in lacZ transcripts and not cyclin E transcripts. Their results led Hannon and his colleagues to suggest RNAi degrades target mRNA through a 'sequence-specific nuclease activity'. They termed the nuclease enzyme RISC.[5] Later Devanand Sarkar and his colleagues Prasanna K. Santhekadur and Byoung Kwon Yoo at the Virginia Commonwealth University elucidated the RISC activity and its molecular mechanism in cancer cells and they identified another new component of the RISC, called AEG-1 [47].

Function in RNA interference

The PIWI domain of an Argonaute protein in complex with double-stranded RNA.

Incorporation of siRNA/miRNA

The RNase III Dicer is a critical member of RISC that initiates the RNA interference process by producing double-stranded siRNA or single-stranded miRNA. Enzymatic cleavage of dsRNA within the cell produces the short siRNA fragments of 21-23 nucleotides in length with a two-nucleotide 3' overhang.[6][7] Dicer also processes pre-miRNA, which forms a hairpin loop structure to mimic dsRNA, in a similar fashion. dsRNA fragments are loaded into RISC with each strand having a different fate based on the asymmetry rule phenomenon, the selection of one strand as the guide strand over the other based on thermodynamic stability.[8][9][10][11] The newly generated miRNA or siRNA act as single-stranded guide sequences for RISC to target mRNA for degradation.[12][13]

  • The strand with the less thermodynamically stable 5' end is selected by the protein Argonaute and integrated into RISC.[11][14] This strand is known as the guide strand and targets mRNA for degradation.
  • The other strand, known as the passenger strand, is degraded by RISC.[15]
Part of the RNA interference pathway with the different ways RISC can silence genes via their messenger RNA.

Gene regulation

AGO2 (grey) in complex with a microRNA (light blue) and its target mRNA (dark blue)

Major proteins of RISC, Ago2, SND1, and AEG-1, act as crucial contributors to the gene silencing function of the complex.[16]

RISC uses the guide strand of miRNA or siRNA to target complementary 3'-untranslated regions (3'UTR) of mRNA transcripts via Watson-Crick base pairing, allowing it to regulate gene expression of the mRNA transcript in a number of ways.[17][1]

mRNA degradation

The most understood function of RISC is degradation of target mRNA which reduces the levels of transcript available to be translated by ribosomes. The endonucleolytic cleavage of the mRNA complementary to the RISC's guide strand by Argonaute protein is the key to RNAi initiation.[18] There are two main requirements for mRNA degradation to take place:

  • a near-perfect complementary match between the guide strand and target mRNA sequence, and,
  • a catalytically active Argonaute protein, called a 'slicer', to cleave the target mRNA.[1]

There are two major pathways of mRNA degradation once cleavage has occurred. Both are initiated through degradation of the mRNA's poly(A) tail, resulting in removal of the mRNA's 5' cap.

Translational repression

RISC can modulate the loading of ribosome and accessory factors in translation to repress expression of the bound mRNA transcript. Translational repression only requires a partial sequence match between the guide strand and target mRNA.[1]

Translation can be regulated at the initiation step by:

Translation can be regulated at post-initiation steps by:

  • peptide degradation,
  • promoting premature termination of translation ribosomes,[21] or,
  • slowing elongation.[22]

There is still speculation on whether translational repression via initiation and post-initiation is mutually exclusive.

Heterochromatin formation

Some RISCs are able to directly target the genome by recruiting histone methyltransferases to form heterochromatin at the gene locus, silencing the gene. These RISCs take the form of a RNA-induced transcriptional silencing complex (RITS). The best studied example is with the yeast RITS.[1][23][24]

RITS has been shown to direct heterochromatin formation at centromeres through recognition of centromeric repeats. Through base-pairing of siRNA (guide strand) to target chromatin sequences, histone-modifying enzymes can be recruited.[25]

The mechanism is not well understood; however, RITS degrade nascent mRNA transcripts. It has been suggested this mechanism acts as a 'self-reinforcing feedback loop' as the degraded nascent transcripts are used by RNA-dependent RNA polymerase (RdRp) to generate more siRNAs.[26]

In Schizosaccharomyces pombe and Arabidopsis, the processing of dsRNA targets into siRNA by Dicer RNases can initiate a gene silencing pathway by heterochromatin formation. An Argonaute protein known as AGO4 interacts with the small RNAs that define heterochromatic sequences. A histone methyl transferase (HMT), H3K9, methylates histone H3 and recruits chromodomain proteins to the methylation sites. DNA methylation maintains the silencing of genes as the heterochromatin sequences can be established or spread.[27]

DNA elimination

The siRNA generated by RISCs seem to have a role in degrading DNA during somatic macronucleus development in protozoa Tetrahymena. It is similar to the epigenetic control of heterochromatin formation and is implied as a defense against invading genetic elements.[27]

Similar to heterochromatin formation in S. pombe and Arabidopsis, a Tetrahymena  protein related to the Argonaute family, Twi1p, catalyzes DNA elimination of target sequences known as internal elimination sequences (IESs). Using methyltransferases and chromodomain proteins, IESs are heterochromatized and eliminated from the DNA.[27]

RISC-associated proteins

The complete structure of RISC is still unsolved. Many studies have reported a range of sizes and components for RISC but it is not entirely sure whether this is due to there being a number of RISC complexes or due to the different sources that different studies use.[28]

Table 1: Complexes implicated in RISC assembly and function Based on table by Sontheimer (2005)[28]
Complex Source Known/apparent components Estimated size Apparent function in RNAi pathway
Dcr2-R2D2[29] D. melanogaster S2 cells Dcr2, R2D2 ~250 kDa dsRNA processing, siRNA binding
RLC (A)[30][31] D. melanogaster embryos Dcr2, R2D2 NR dsRNA processing, siRNA binding, precursor to RISC
Holo-RISC[30][31] D. melanogaster embryos Ago 2, Dcr1, Dcr2, Fmr1/Fxr, R2D2, Tsn, Vig ~80S Target-RNA binding and cleavage
RISC[32] D. melanogaster S2 cells Ago2 ~140 kDa Target-RNA binding and cleavage
Fmr1-associated complex[33] D. melanogaster S2 cells L5, L11, 5S rRNA, Fmr1/Fxr, Ago2, Dmp68 NR Possible target-RNA binding and cleavage
Minimal RISC[34][35][36][37] HeLa cells eIF2C1 (Ago1) or eIF2C2 (Ago2) ~160 kDa Target-RNA binding and cleavage
miRNP[38][39] HeLa cells eIF2C2 (ago2), Gemin3, Gemin4 ~550 kDa miRNA association, target-RNA binding and cleavage

Ago, Argonaute; Dcr, Dicer; Dmp68, D. melanogaster orthologue of mammalian p68 RNA unwindase; eIF2C1, eukaryotic translation initiation factor 2C1; eIF2C2, eukaryotic translation initiation factor 2C2; Fmr1/Fxr, D. melanogaster orthologue of the fragile-X mental retardation protein; miRNP, miRNA-protein complex; NR, not reported; Tsn, Tudor-staphylococcal nuclease; Vig, vasa intronic gene.

A full-length argonaute protein from the archaea species Pyrococcus furiosus.

Regardless, it is apparent that Argonaute proteins are present and are essential for function. Furthermore, there are insights into some of the key proteins (in addition to Argonaute) within the complex, which allow RISC to carry out its function.

Argonaute proteins

Main page: Biology:Argonaute

Argonaute proteins are a family of proteins found in prokaryotes and eukaryotes. Their function in prokaryotes is unknown but in eukaryotes they are responsible for RNAi.[40] There are eight family members in human Argonautes of which only Argonaute 2 is exclusively involved in targeted RNA cleavage in RISC.[37]

The RISC-loading complex allows the loading of dsRNA fragments (generated by Dicer) to be loaded onto Argonaute 2 (with the help of TRBP) as part of the RNA interference pathway.

RISC-loading complex

The RISC-loading complex (RLC) is the essential structure required to load dsRNA fragments into RISC in order to target mRNA. The RLC consists of dicer, the transactivating response RNA-binding protein (TRBP) and Argonaute 2.

  • Dicer is an RNase III endonuclease which generates the dsRNA fragments to be loaded that direct RNAi.
  • TRBP is a protein with three double-stranded RNA-binding domains.
  • Argonaute 2 is an RNase and is the catalytic centre of RISC.

Dicer associates with TRBP and Argonaute 2 to facilitate the transfer of the dsRNA fragments generated by Dicer to Argonaute 2.[41][42]

More recent research has shown the human RNA helicase A could help facilitate the RLC.[43]

Other proteins

Recently identified members of RISC are SND1 and MTDH.[44] SND1 and MTDH are oncogenes and regulate various gene expression.[45]

Table 2: Biochemically documented proteins associated with RISC Based on the table by Sontheimer (2005)[28]
Protein Species the protein is found
Dcr1[30] D. melanogaster
Dcr2[29][30][31] D. melanogaster
R2D2[30][31] D. melanogaster
Ago2[30][46][32][33] D. melanogaster
Dmp68[33] D. melanogaster
Fmr1/Fxr[30][47][33] D. melanogaster
Tsn[30][48] D. melanogaster
Vig[30][47] D. melanogaster
Polyribosomes, ribosome components[5][30][46][33][49] D. melanogaster, T. brucei
eIF2C1 (Ago1)[34] H. sapiens
eIF2C2 (Ago2)[34][35][37][39] H. sapiens
Gemin3[38][39] H. sapiens
Gemin4[38][39] H. sapiens

Ago, Argonaute; Dcr, Dicer; Dmp68, D. melanogaster orthologue of mammalian p68 RNA unwindase; eIF2C1, eukaryotic translation initiation factor 2C1; eIF2C2, eukaryotic translation initiation factor 2C2; Fmr1/Fxr, D. melanogaster orthologue of the fragile-X mental retardation protein; Tsn, Tudor-staphylococcal nuclease; Vig, vasa intronic gene.

Binding of mRNA

Diagram of RISC activity with miRNAs

It is as yet unclear how the activated RISC complex locates the mRNA targets in the cell, though it has been shown that the process can occur in situations outside of ongoing protein translation from mRNA.[50]

Endogenously expressed miRNA in metazoans is usually not perfectly complementary to a large number of genes and thus, they modulate expression via translational repression.[51][52] However, in plants, the process has a much greater specificity to target mRNA and usually each miRNA only binds to one mRNA. A greater specificity means mRNA degradation is more likely to occur.[53]

See also

References

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  2. ↑ 2.0 2.1 "Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight?". Nature Reviews Genetics 9 (2): 102–114. 2008. doi:10.1038/nrg2290. PMID 18197166. https://escholarship.mcgill.ca/concern/articles/cz30pz55f. 
  3. ↑ 3.0 3.1 "Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans". Nature 391 (6669): 806–811. 1998. doi:10.1038/35888. PMID 9486653. Bibcode: 1998Natur.391..806F. 
  4. ↑ Watson, James D. (2008). Molecular Biology of the Gene. San Francisco, CA: Cold Spring Harbor Laboratory Press. pp. 641–648. ISBN 978-0-8053-9592-1. 
  5. ↑ 5.0 5.1 5.2 "An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells". Nature 404 (6775): 293–296. 2000. doi:10.1038/35005107. PMID 10749213. Bibcode: 2000Natur.404..293H. 
  6. ↑ "RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals". Cell 101 (1): 25–33. 2000. doi:10.1016/S0092-8674(00)80620-0. PMID 10778853. 
  7. ↑ "The contributions of dsRNA structure to Dicer specificity and efficiency". RNA 11 (5): 674–682. 2005. doi:10.1261/rna.7272305. PMID 15811921. 
  8. ↑ Hutvagner, Gyorgy (2005). "Small RNA asymmetry in RNAi: Function in RISC assembly and gene regulation" (in en). FEBS Letters 579 (26): 5850–5857. doi:10.1016/j.febslet.2005.08.071. ISSN 1873-3468. PMID 16199039. 
  9. ↑ "Asymmetry in the assembly of the RNAi enzyme complex". Cell 115 (2): 199–208. 2003. doi:10.1016/S0092-8674(03)00759-1. PMID 14567917. 
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  14. ↑ Preall, Jonathan B.; He, Zhengying; Gorra, Jeffrey M.; Sontheimer, Erik J. (2006-03-07). "Short Interfering RNA Strand Selection Is Independent of dsRNA Processing Polarity during RNAi in Drosophila" (in English). Current Biology 16 (5): 530–535. doi:10.1016/j.cub.2006.01.061. ISSN 0960-9822. PMID 16527750. 
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  43. ↑ "Structural insights into RISC assembly facilitated by dsRNA-binding domains of human RNA helices A (DHX9)". Nucleic Acids Research 41 (5): 3457–3470. 2013. doi:10.1093/nar/gkt042. PMID 23361462. 
  44. ↑ "Increased RNA-induced silencing complex (RISC) activity contributes to hepatocellular carcinoma". Hepatology 53 (5): 1538–1548. 2011. doi:10.1002/hep.24216. PMID 21520169. 
  45. ↑ "Astrocyte elevated gene (AEG-1): a multifunctional regulator of normal and abnormal physiology". Pharmacology & Therapeutics 130 (1): 1–8. 2011. doi:10.1016/j.pharmthera.2011.01.008. PMID 21256156. 
  46. ↑ 46.0 46.1 Cite error: Invalid <ref> tag; no text was provided for refs named Hammond2001
  47. ↑ 47.0 47.1 Cite error: Invalid <ref> tag; no text was provided for refs named Caudy2002
  48. ↑ Cite error: Invalid <ref> tag; no text was provided for refs named Caudy2003
  49. ↑ "An siRNA ribonucleoprotein is found associated with polyribosomes in Trypanosoma brucei". RNA 9 (7): 802–808. 2003. doi:10.1261/rna.5270203. PMID 12810914. 
  50. ↑ "mRNA translation is not a prerequisite for small interfering RNA-mediated mRNA cleavage". Differentiation 73 (6): 287–293. 2005. doi:10.1111/j.1432-0436.2005.00029.x. PMID 16138829. 
  51. ↑ "Anti-viral RNA silencing: do we look like plants?". Retrovirology 3: 3. 2006. doi:10.1186/1742-4690-3-3. PMID 16409629. 
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Further reading

External links



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