Biology:ERG5
| Sterol 22-desaturase | |||||||
|---|---|---|---|---|---|---|---|
| Identifiers | |||||||
| Organism | |||||||
| Symbol | ERG5 | ||||||
| Alt. symbols | CYP61A1 | ||||||
| Entrez | 855029 | ||||||
| HomoloGene | 20212 | ||||||
| RefSeq (mRNA) | NM_001182511.1 | ||||||
| RefSeq (Prot) | NP_013728.1 | ||||||
| UniProt | P54781 | ||||||
| Other data | |||||||
| EC number | 1.14.19.41 | ||||||
| Chromosome | XIII: 0.3 - 0.3 Mb | ||||||
| |||||||
ERG5 or Sterol 22-desaturase is a cytochrome P450 enzyme in the ergosterol biosynthesis pathway of fungi Saccharomyces cerevisiae (Baker's yeast, a model organism), with the CYP Symbol CYP61A1.[1] CYP61A1 is one of only three P450 enzyme found in baker's yeast, the other two are CYP51F1 and CYP56A1.[2] The ortholog in Schizosaccharomyces pombe (fission yeast, the second sequenced model fungi), was named CYP61A3 for historical reasons, and is only one of two P450 enzyme found with CYP51F1.[3] ERG5 catalyzes the C22-C23 double bond formation on the sterol side chain of ergostatrienol to convert it into ergostatetraenol, then the C24 double bond of ergostatetrenol will be hydrogenation reduced into ergosterol by ERG4.[4]
Ortholog
Most fungi have only one CYP61 gene, and CYP61 global distributed in fungi.[5][6] There are some of the ortholog found in early genome sequenced organism:[7]
| CYP symbol | Species | UniProt/EMBL |
|---|---|---|
| CYP61A1 | Saccharomyces cerevisiae | P54781 |
| CYP61A2 | Candida albicans | A0A1B3B2L4 |
| CYP61A3 | Schizosaccharomyces pombe | O13820 |
| CYP61A4 | Botrytis cinerea | AL111744 |
| CYP61A5 | Neurospora crassa |
References
- ↑ "Global analysis of protein expression in yeast". Nature 425 (6959): 737–41. October 2003. doi:10.1038/nature02046. PMID 14562106. Bibcode: 2003Natur.425..737G.
- ↑ "Cytochrome P450 diversity in the tree of life". Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 1866 (1): 141–154. January 2018. doi:10.1016/j.bbapap.2017.05.003. PMID 28502748.
- ↑ "Dynamic repertoire of a eukaryotic transcriptome surveyed at single-nucleotide resolution". Nature 453 (7199): 1239–43. June 2008. doi:10.1038/nature07002. PMID 18488015. Bibcode: 2008Natur.453.1239W.
- ↑ "Characterization of Saccharomyces cerevisiae CYP61, sterol delta22-desaturase, and inhibition by azole antifungal agents". The Journal of Biological Chemistry 272 (15): 9986–8. April 1997. doi:10.1074/JBC.272.15.9986. PMID 9092539.
- ↑ "Fungal cytochrome p450 monooxygenases: their distribution, structure, functions, family expansion, and evolutionary origin". Genome Biology and Evolution 6 (7): 1620–34. June 2014. doi:10.1093/gbe/evu132. PMID 24966179.
- ↑ "Systematic and searchable classification of cytochrome P450 proteins encoded by fungal and oomycete genomes". BMC Genomics 13: 525. October 2012. doi:10.1186/1471-2164-13-525. PMID 23033934.
- ↑ "Note on P450 evolution in yeasts and early eukaryotes.". The Cytochrome P450 Homepage (The University of Tennessee Health Science Center) 4 (1): 59–65. 25 March 1999. doi:10.1186/1479-7364-4-1-59. PMID 19951895. PMC 3500189. https://drnelson.uthsc.edu/yeastP450.evol.html.
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