Biology:Exonuclease VII
| Exonuclease VII, large subunit | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| Symbol | Exonuc_VII_L | ||||||||
| Pfam | PF02601 | ||||||||
| InterPro | IPR020579 | ||||||||
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| Exonuclease VII small subunit | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 crystal structure of exodeoxyribonuclease vii small subunit (np_881400.1) from bordetella pertussis at 2.40 a resolution | |||||||||
| Identifiers | |||||||||
| Symbol | Exonuc_VII_S | ||||||||
| Pfam | PF02609 | ||||||||
| InterPro | IPR003761 | ||||||||
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The enzyme exodeoxyribonuclease VII (EC 3.1.11.6, Escherichia coli exonuclease VII, E. coli exonuclease VII, endodeoxyribonuclease VII, exodeoxyribonuclease VII) is a bacterial exonuclease enzyme.[1][2] It is composed of two nonidentical subunits; one large subunit and 4 small ones.[3] that catalyses exonucleolytic cleavage in either 5′- to 3′- or 3′- to 5′-direction to yield nucleoside 5′-phosphates. The large subunit also contains an N-terminal OB-fold domain that binds to nucleic acids.
The widely used quinolone antibiotics induce DNA damage in bacterial cells by trapping DNA gyrase (an essential type II topoisomerase), leading to blocked gyrase cleavage complexes.[4] Exonuclease VII participates in repairing such DNA damage by resolving the trapped cleavage complexes.[4]
When Escherichia coli bacteria recA mutants are UV-irradiated, “reckless” DNA degradation occurs that involves exonuclease VII.[5]
References
- ↑ Chase, J.W.; Richardson, C.C. (1974). "Ribonuclease VII of Escherichia coli". J. Biol. Chem. 249 (14): 4545–4552. doi:10.1016/S0021-9258(19)42453-8. PMID 4602029.
- ↑ Chase, J.W.; Richardson, C.C. (1974). "Exonuclease VII of Escherichia coli". J. Biol. Chem. 249 (14): 4553–4561. doi:10.1016/S0021-9258(19)42454-X. PMID 4602030.
- ↑ "Subunit structure of Escherichia coli exonuclease VII". J. Biol. Chem. 257 (15): 8799–805. August 1982. doi:10.1016/S0021-9258(18)34201-7. PMID 6284744.
- ↑ 4.0 4.1 "Exonuclease VII repairs quinolone-induced damage by resolving DNA gyrase cleavage complexes". Sci Adv 7 (10). March 2021. doi:10.1126/sciadv.abe0384. PMID 33658195. Bibcode: 2021SciA....7..384H.
- ↑ "Exonuclease VII is involved in "reckless" DNA degradation in UV-irradiated Escherichia coli". Mutat Res 750 (1–2): 96–104. January 2013. doi:10.1016/j.mrgentox.2012.10.005. PMID 23123979. Bibcode: 2013MRGTE.750...96R.
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