Biology:Glucose-1-phosphate thymidylyltransferase
| Glucose-1-phosphate thymidylyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 glucose-1-phosphate thymidylyltransferase tetramer, E.Coli | |||||||||
| Identifiers | |||||||||
| EC number | 2.7.7.24 | ||||||||
| CAS number | 9026-03-3 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24) is an enzyme that catalyzes the chemical reaction
- REDIRECT Template:Chemical reaction
The enzyme characterised from Pseudomonas aeruginosa and Streptococcus faecalis combines thymidine triphosphate and glucose 1-phosphate to give thymidine diphosphate glucose, with pyrophosphate (PPi) as a byproduct.[1][2] The mechanism of the reaction has been studied in Escherichia coli,[3] where it is part of the biosynthetic pathway to L-rhamnose.[4]
Nomenclature
This enzyme is a transferase, specifically one transferring phosphorus-containing nucleotide groups (nucleotidyltransferases). The systematic name of this enzyme class is dTTP:alpha-D-glucose-1-phosphate thymidylyltransferase. Other names in common use include:[5]
- glucose 1-phosphate thymidylyltransferase,
- dTDP-glucose synthase, dTDP-glucose pyrophosphorylase,
- thymidine diphosphoglucose pyrophosphorylase,
- thymidine diphosphate glucose pyrophosphorylase, and
- TDP-glucose pyrophosphorylase.
Structural studies
As of late 2007, 19 structures have been solved for this class of enzymes, with PDB accession codes 1FXO, 1FZW, 1G0R, 1G1L, 1G23, 1G2V, 1G3L, 1H5R, 1H5S, 1H5T, 1IIM, 1IIN, 1LVW, 1MC3, 1MP3, 1MP4, 1MP5, 2GGO, and 2GGQ.
References
- ↑ "The enzymic synthesis of thymidine-linked sugars. I. Thymidine diphosphate glucose". J. Biol. Chem. 236 (6): 1791–4. June 1961. doi:10.1016/S0021-9258(19)63304-1. PMID 13753209.
- ↑ "The enzymatic synthesis of thymidine diphosphate glucose and its conversion to thymidine diphosphate rhamnose". J. Biol. Chem. 236 (6): 1780–5. June 1961. doi:10.1016/S0021-9258(19)63302-8. PMID 13733719.
- ↑ Zuccotti, Simone; Zanardi, Davide; Rosano, Camillo; Sturla, Laura; Tonetti, Michela; Bolognesi, Martino (2001). "Kinetic and crystallographic analyses support a sequential-ordered bi bi catalytic mechanism for Escherichia coli glucose-1-phosphate thymidylyltransferase". Journal of Molecular Biology 313 (4): 831–843. doi:10.1006/jmbi.2001.5073. PMID 11697907.
- ↑ "Biosynthesis of O-antigens: genes and pathways involved in nucleotide sugar precursor synthesis and O-antigen assembly". Carbohydr. Res. 338 (23): 2503–19. 2003. doi:10.1016/j.carres.2003.07.009. PMID 14670712.
- ↑ Enzyme 2.7.7.24 at KEGG Pathway Database.
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