Biology:Tre recombinase
From HandWiki
Tre recombinase is an experimental enzyme that in lab tests has removed DNA inserted by HIV from infected cells.[1] Through selective mutation, Cre recombinase which recognizes loxP sites are modified to identify HIV long terminal repeats (loxLTR) instead. As a result, instead of performing Cre-Lox recombination, the new enzyme performs recombination at HIV provirus sites.[2] The structure of Tre in complex with loxLTR has been resolved (PDB: 5U91), allowing for analyzing the roles of individual mutations.[3]
References
- ↑ Sarkar, Indrani; Hauber, Ilona; Hauber, Joachim; Buchholz, Frank (2007). "HIV-1 proviral DNA excision using an evolved recombinase". Science 316 (5833): 1912–15. doi:10.1126/science.1141453. PMID 17600219. Bibcode: 2007Sci...316.1912S.
- ↑ Hauber, Ilona; Hofmann-Sieber, Helga; Chemnitz, Jan et al. (September 26, 2013). "Highly Significant Antiviral Activity of HIV-1 LTR-Specific Tre-Recombinase in Humanized Mice". PLOS Pathogens 9 (9): e1003587. doi:10.1371/journal.ppat.1003587. PMID 24086129.
- ↑ Meinke, G; Karpinski, J; Buchholz, F; Bohm, A (19 September 2017). "Crystal structure of an engineered, HIV-specific recombinase for removal of integrated proviral DNA.". Nucleic Acids Research 45 (16): 9726–9740. doi:10.1093/nar/gkx603. PMID 28934476.
External links
- Minkel, JR (June 28, 2007). "Designer enzyme cuts HIV out of infected cells". Scientific American. http://www.sciam.com/article.cfm?articleID=737AB56E-E7F2-99DF-382B756D1860EACA&chanID=sa003.
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