Biology:Retinol-binding protein

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Not to be confused with RNA-binding proteins, also abbreviated as RBPs.
Retinol-binding protein (plasma), Human.
Short description: Family of proteins that bind retinol

Retinol-binding proteins (RBP) are a family of proteins with diverse functions. They are carrier proteins that bind retinol. Assessment of retinol-binding protein is used to determine visceral protein mass in health-related nutritional studies.

Retinol and retinoic acid play crucial roles in the modulation of gene expression and overall development of an embryo. However, deficit or excess of either one of these substances can cause early embryo mortality or developmental malformations. Regulation of transport and metabolism of retinol necessary for a successful pregnancy is accomplished via RBP. Retinol-binding proteins have been identified within the uterus, embryo, and extraembryonic tissue of the bovine, ovine, and porcine, clearly indicating that RBP plays a role in proper retinol exposure to the embryo and successful transport at the maternal-fetal interface. Further research is necessary to determine the exact effects of poor RBP expression on pregnancy and threshold levels for said expression.

Genes

  • Cellular: RBP1, RBP2, RBP5, RBP7
  • Interstitial: RBP3
  • Plasma: RBP4

RBP in pregnancy

Retinol plays a crucial role in the growth and differentiation of various body tissues, and it has been previously characterized that embryos are extremely sensitive to alterations in retinol concentration that can lead to spontaneous abortion and malformations occurring during development.[1][2] Within a mature animal, retinol is transported from the liver via the circulatory system while bound to RBP to the desired target tissue. RBP is also bound to a carrier protein, transthyretin.[3] The process by which RBP releases retinol for cellular availability is still unknown and not concisely determined.[4][5][6]

Sites of synthesis

Traditionally, RBP is synthesized within the liver with secretion being dependent upon retinol concentrations. However, the concentrations levels do not appear to have an effect upon transcription of RBP messenger RNA (mRNA) which remains constant.[7][8] Literature reveals that the bovine endometrium has also been identified as a location of RBP synthesis, as well as, the conceptus and extraembryonic tissues of various livestock species.[9][10][11][12]

Types

  1. Plasma retinol-binding protein, the retinol transport vehicle in serum.[13]
  2. CRBP I/II, cellular-binding proteins involved in transport of retinol and metabolites into retinyl esters for storage or into retinoic acid.[14]
  3. CRABPs, cellular retinoic acid–binding proteins capable of binding retinol and retinoic acid with high affinity.[15][16][17] It has also been characterized that CRABPs are involved in many aspects of the retinoic acid signaling pathway such as the regulation and availability of retinoic acid to nuclear receptors.[18]

Presence in livestock species during gestation

Bovine/Ovine

RBP, identical to that found in plasma has been identified in the placental tissues of both the ovine and the bovine, suggesting that RBP may be highly involved in retinol transport and metabolism during pregnancy.[2][10] However, exact timing of expression had been yet to be identified. An antiserum specific for bovine conceptus RBP and immunohistochemistry has been utilized to identify the presence of RBP at different stages of early pregnancy.[2] Strong immunostaining and hybridization were observed in the trophectoderm of tubular, but not spherical blastocysts at day 13. RBP mRNA was localized to epithelial cells of the chorion, allantois, and amnion at day 45 of pregnancy.[2] Lastly, RBP mRNA was detected in the cotyledons, the fetal contribution to the placenta and the site of attachment to the uterine epithelium for fetal/maternal exchange.[2] Expression of RBP in developing conceptuses, extraembryonic membranes, and at the fetal-maternal interface indicate that there may be some regulation of retinol transport and metabolism that occurs due to RBP by the extraembryonic membranes.[10] Within the uterus of pregnant bovines, it has been found that RBP synthesis in the luminal and glandular epithelium is quite similar to that of a cyclic animal's; however upon reaching day 17 of the estrous cycle, levels of RBP remain constant and continue to gradually rise throughout gestation.[19] It has also been suggested that ovarian steroids may play a role in regulating uterine RBP expression.[19]

Porcine

All three previously mentioned types of retinol-binding proteins (RBP, CRBP, CRABP) have been identified within the porcine placenta during pregnancy via immunohistochemistry.[14] As previously mentioned, retinol and retinoic acid are modulators of gene expression and are necessary for the proper development and growth of a conceptus.[14] Porcine exhibit a diffuse type placenta that has areolar-gland subunits which allows for transport of larger molecules between dam and fetus. RBP and CRBP have been identified in the endometrial glands and areolar trophoblasts, suggesting that RBP is crucial in transport of retinol from the gland to the trophectoderm of the conceptus.[14] RBP expression has also been identified within the yolk sac, myometrium, oviduct, and numerous other fetal tissues.[9]

See also

  • STRA6 (Vitamin A receptor)

References

  1. ↑ "Multiple functions of vitamin A". Physiological Reviews 64 (3): 873–937. July 1984. doi:10.1152/physrev.1984.64.3.873. PMID 6377341. 
  2. ↑ 2.0 2.1 2.2 2.3 2.4 "Expression and cellular localization of retinol-binding protein messenger ribonucleic acid in bovine blastocysts and extraembryonic membranes". Biology of Reproduction 49 (2): 393–400. August 1993. doi:10.1095/biolreprod49.2.393. PMID 8373966. 
  3. ↑ "The role of hepatic fat-storing (stellate) cells in retinoid metabolism". Hepatology 7 (6): 1368–71. 1987. doi:10.1002/hep.1840070630. PMID 3315935. 
  4. ↑ "Comparison of the rate of uptake and biologic effects of retinol added to human keratinocytes either directly to the culture medium or bound to serum retinol-binding protein". The Journal of Investigative Dermatology 97 (2): 298–304. August 1991. doi:10.1111/1523-1747.ep12480562. PMID 2071940. 
  5. ↑ "Localization of cellular retinol-binding protein and retinol-binding protein in cells comprising the blood-brain barrier of rat and human". Proceedings of the National Academy of Sciences of the United States of America 87 (11): 4265–9. June 1990. doi:10.1073/pnas.87.11.4265. PMID 2190219. Bibcode: 1990PNAS...87.4265M. 
  6. ↑ "Retinol uptake from retinol-binding protein (RBP) by liver parenchymal cells in vitro does not specifically depend on its binding to RBP". Biochemistry 32 (7): 1727–33. February 1993. doi:10.1021/bi00058a005. PMID 8439537. 
  7. ↑ "Retinoids, retinoid-binding proteins, and retinyl palmitate hydrolase distributions in different types of rat liver cells". Journal of Lipid Research 26 (10): 1241–51. October 1985. doi:10.1016/S0022-2275(20)34272-3. PMID 4067418. 
  8. ↑ "Retinol-binding protein messenger RNA levels in the liver and in extrahepatic tissues of the rat". Journal of Lipid Research 27 (2): 166–71. February 1986. doi:10.1016/S0022-2275(20)38843-X. PMID 3754273. 
  9. ↑ 9.0 9.1 "Retinol-binding protein: immunolocalization of protein and abundance of messenger ribonucleic acid in conceptus and maternal tissues during pregnancy in pigs". Biology of Reproduction 50 (5): 1126–35. May 1994. doi:10.1095/biolreprod50.5.1126. PMID 8025169. 
  10. ↑ 10.0 10.1 10.2 "Characterization and immunolocalization of bovine uterine retinol-binding protein". Biology of Reproduction 47 (6): 1099–104. December 1992. doi:10.1095/biolreprod47.6.1099. PMID 1493174. 
  11. ↑ "Purification and immunolocalization of ovine placental retinol-binding protein". Biology of Reproduction 46 (1): 23–9. January 1992. doi:10.1095/biolreprod46.1.23. PMID 1547313. 
  12. ↑ "Delivery of vitamin A from parenteral nutrition solutions in neonates". Journal of Paediatrics and Child Health 27 (3): 180–3. June 1991. doi:10.1111/j.1440-1754.1991.tb00382.x. PMID 1909535. 
  13. ↑ "A sustained elevation in retinoic acid receptor-beta 2 mRNA and protein occurs during retinoic acid-induced fetal dysmorphogenesis". Mechanisms of Development 45 (3): 243–53. March 1994. doi:10.1016/0925-4773(94)90011-6. PMID 8011556. 
  14. ↑ 14.0 14.1 14.2 14.3 "Immunohistochemical localization of retinoid binding proteins at the materno-fetal interface of the porcine epitheliochorial placenta". Biology of Reproduction 64 (1): 60–8. January 2001. doi:10.1095/biolreprod64.1.60. PMID 11133659. 
  15. ↑ "Structure/function of cytoplasmic vitamin A-binding proteins". Annual Review of Nutrition 16: 205–34. 1996. doi:10.1146/annurev.nutr.16.1.205. PMID 8839926. 
  16. ↑ "Enzymes and binding proteins affecting retinoic acid concentrations". The Journal of Steroid Biochemistry and Molecular Biology 53 (1–6): 497–502. June 1995. doi:10.1016/0960-0760(95)00096-i. PMID 7626500. 
  17. ↑ Napoli, J.L. (1996). "Retinoic acid biosynthesis and metabolism". The FASEB Journal 10 (9): 993–1001. doi:10.1096/fasebj.10.9.8801182. PMID 8801182. 
  18. ↑ "Nuclear import of cellular retinoic acid-binding protein type I in mouse embryonic cells". Mechanisms of Development 58 (1–2): 27–38. August 1996. doi:10.1016/s0925-4773(96)00554-0. PMID 8887314. 
  19. ↑ 19.0 19.1 "Bovine endometrial retinol-binding protein secretion, messenger ribonucleic acid expression, and cellular localization during the estrous cycle and early pregnancy". Biology of Reproduction 57 (6): 1445–50. December 1997. doi:10.1095/biolreprod57.6.1445. PMID 9408253. 

Further reading

External links



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