Biology:mir-223

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Short description: Mir-223
mir-223
MiR-223 secondary structure.png
miR-223 microRNA secondary structure and sequence conservation
Identifiers
Symbolmir-223
RfamRF00664
miRBase familyMIPF0000067
NCBI Gene407008
HGNC31603
OMIM300694
Other data
RNA typemicroRNA
Domain(s)Eukaryota; Euteleostomi
PDB structuresPDBe

In molecular biology MicroRNA-223 (miR-223) is a short RNA molecule. MicroRNAs function to regulate the expression levels of other genes by several mechanisms. miR-223 is a hematopoietic specific microRNA with crucial functions in myeloid lineage development.[1][2] It plays an essential role in promoting granulocytic differentiation[2][3] while also being associated with the suppression of erythrocytic differentiation.[4] miR-223 is commonly repressed in hepatocellular carcinoma[5] and leukemia.[6][7][8][9] Higher expression levels of miRNA-223 are associated with extranodal marginal-zone lymphoma of mucosa-associated lymphoid tissue of the stomach[10] and recurrent ovarian cancer.[11] In some cancers the microRNA-223 down-regulation is correlated with higher tumor burden, disease aggressiveness, and poor prognostic factors.[7] MicroRNA-223 is also associated with rheumatoid arthritis,[12] sepsis,[13] type 2 diabetes,[14] and hepatic ischemia.[15]

Characterization

MicroRNA-223 was initially identified bioinformatically and it was subsequently characterized as part of the haematopoietic system.[2] Its gene resembles a myeloid gene and it could be driven by the PU.1 and C/EBPα proteins which are myeloid transcription factors.[16]

MicroRNA-223 selectively targets distinct populations of transcripts harboring AU-rich elements. More specifically, it was validated that the RhoB mRNA is a bona fide miR-223 target.[17] miR-223 also regulates cyclin E activity by modulating expression of the FBXW7 protein. In particular, overexpression of miR-223 reduces FBXW7 mRNA levels while increasing endogenous cyclin E protein and activity levels.[18]

Role in hematopoiesis

The role of miR-223 in hematopoiesis has been extensively analyzed in the past few years. During granulopoiesis miR-223 acts as fine-tuner of granulocytic differentiation, maturation, and function.[19] More specifically, human granulocytic differentiation is controlled by a regulatory circuitry involving miR-223 and two transcriptional factors, NFIA and C/EBPα. These two factors compete for binding: NFI-A maintains miR-223 at low levels whereas C/EBPα upregulates miR-223 expression. The competition by C/EBPα and the granulocytic differentiation are favored by a negative-feedback loop in which miR-223 represses NFI-A translation.[20]

Analysis of expression profiles indicate that miR-223 expression decreases as cells mature during monocytic, erythroid, and mast cell differentiation.[2][19] miR-223 down-regulation during erythropoiesis is required for erythrocyte proliferation and differentiation at progenitor and precursor level.[19] This down-modulation promotes erythropoiesis favoring translation of the key functional protein LMO2 resulting in reversible regulation of erythroid and megakaryocytic differentiation.[4]

MicroRNA-223 also plays an essential role during osteoclast differentiation. More specifically, miR-223 expression suppresses the differentiation of osteoclast precursors into osteoclast thus making it a potential viable therapeutic target for a range of bone metabolic disorders with excess osteoclast activity.[21]

Involvement in disease

Cancer

MicroRNA-223 is commonly repressed in hepatocellular carcinoma,[5] chronic lymphocytic leukemia,[7] acute lymphoblastic leukemia,[8] acute myeloid leukemia,[6][9] gastric MALT lymphoma,[10] and recurrent ovarian cancer.[11]

Integrative analysis in hepatocellular carcinoma implicates Stathmin 1 (STMN1) as a downstream target of miR-223. Furthermore, miR-223 could suppress the luciferase activity in reporter construct containing the STMN1 3' untranslated region.[5] The reduced expressions of miR-223 may predispose to the development of hepatocellular carcinoma via the widespread induction of chromosomal instability by STMN1.[5]

MicroRNA-223 blocks the translation of E2F1 leading to inhibition of cell-cycle progression followed by myeloid differentiation.[9] In acute myeloid leukemia (AML), miR-223 is down-regulated thus leading to E2F1 overexpression. The overexpressed E2F1 could bind to the miR-223 promoter and in turn lead to a further decrease in miR-223 expression through a negative feedback loop followed by myeloid cell-cycle progression at the expense of differentiation.[6] Overexpression of E2F1 has been shown to be an oncogenic event that predisposes cells to transformation. While there is some indication of the miR-223 role in AML there is still little known about this microRNA function in chronic lymphocytic and acute lymphoblastic leukemia. Nevertheless, MicroRNA-223 expression levels decreased significantly with the progression of these two diseases thus associating miR-223 down-regulation with higher tumor burden, disease aggressiveness, and poor prognostic factors.[7][8]

Gastric MALT lymphoma and recurrent ovarian cancer are associated with higher levels of MicroRNA-223 expression[10][11] making them a potential biomarker.

Rheumatoid arthritis

MicroRNA-223 is overexpressed in the T-lymphocytes cells of rheumatoid arthritis patients suggesting that its expression in this cell type could contribute to the etiology of the disease.[12]

Sepsis

There is some evidence that MicroRNA-223 and MicroRNA-146a are significantly reduced in septic patients compared with systemic inflammatory response syndrome (SIRS) patients and/or healthy controls.[13] This suggests that miR-223 can be used as a biomarker for distinguishing sepsis from SIRS.

Diabetes

Quantitative miRNA expression analyses revealed that miR-223 was consistently upregulated in the insulin-resistant hearts of patients with type 2 diabetes. This effect was associated with miR-223 role in Glut4 regulation and glucose metabolism.[14]

Hepatic ischemia

A recent study concluded that hepatic ischemia/reperfusion injury might be another form of liver disease that is associated with the alteration in miR-223 expression.[15] Correlation analysis revealed that hepatic miR-223 expression levels are significantly positively correlated with the serum markers of hepatic ischemia. Further, prediction assay of miRNA targets mRNA, acyl-CoA synthetase long-chain family member 3, ephrin A1, and ras homolog gene family member B were predicted to be downstream targets of miR-223.

See also

References

  1. ↑ "miR-223 and miR-142 attenuate hematopoietic cell proliferation, and miR-223 positively regulates miR-142 through LMO2 isoforms and CEBP-β.". Cell Res 20 (10): 1158–69. 2010. doi:10.1038/cr.2010.134. PMID 20856265. 
  2. ↑ 2.0 2.1 2.2 2.3 "Regulation of progenitor cell proliferation and granulocyte function by microRNA-223.". Nature 451 (7182): 1125–9. 2008. doi:10.1038/nature06607. PMID 18278031. Bibcode: 2008Natur.451.1125J. 
  3. ↑ "Epigenetic silencing of the myelopoiesis regulator microRNA-223 by the AML1/ETO oncoprotein.". Cancer Cell 12 (5): 457–66. 2007. doi:10.1016/j.ccr.2007.09.020. PMID 17996649. 
  4. ↑ 4.0 4.1 "MicroRNA-223 reversibly regulates erythroid and megakaryocytic differentiation of K562 cells". J Cell Mol Med 13 (11–12): 4551–9. 2009. doi:10.1111/j.1582-4934.2008.00585.x. PMID 19017354. 
  5. ↑ 5.0 5.1 5.2 5.3 "MicroRNA-223 is commonly repressed in hepatocellular carcinoma and potentiates expression of Stathmin1". Gastroenterology 135 (1): 257–69. 2008. doi:10.1053/j.gastro.2008.04.003. PMID 18555017. 
  6. ↑ 6.0 6.1 6.2 "Complexity of miR-223 regulation by CEBPA in human AML". Leuk Res 34 (5): 672–6. 2010. doi:10.1016/j.leukres.2009.11.019. PMID 20018373. 
  7. ↑ 7.0 7.1 7.2 7.3 "microRNA-29c and microRNA-223 down-regulation has in vivo significance in chronic lymphocytic leukemia and improves disease risk stratification". Blood 113 (21): 5237–45. 2009. doi:10.1182/blood-2008-11-189407. PMID 19144983. 
  8. ↑ 8.0 8.1 8.2 "Gene expression profiling identifies a subset of adult T-cell acute lymphoblastic leukemia with myeloid-like gene features and over-expression of miR-223". Haematologica 95 (7): 1114–21. 2010. doi:10.3324/haematol.2009.015099. PMID 20418243. 
  9. ↑ 9.0 9.1 9.2 "Cell-cycle regulator E2F1 and microRNA-223 comprise an autoregulatory negative feedback loop in acute myeloid leukemia". Blood 115 (9): 1768–78. 2010. doi:10.1182/blood-2009-08-240101. PMID 20029046. 
  10. ↑ 10.0 10.1 10.2 "E2A-positive gastric MALT lymphoma has weaker plasmacytoid infiltrates and stronger expression of the memory B-cell-associated miR-223: possible correlation with stage and treatment response". Mod Pathol 23 (11): 1507–17. 2010. doi:10.1038/modpathol.2010.139. PMID 20802470. 
  11. ↑ 11.0 11.1 11.2 "Potential role of miR-9 and miR-223 in recurrent ovarian cancer". Mol Cancer 7: 35. 2008. doi:10.1186/1476-4598-7-35. PMID 18442408. 
  12. ↑ 12.0 12.1 "miR-223 is overexpressed in T-lymphocytes of patients affected by rheumatoid arthritis". Hum Immunol 71 (2): 206–11. 2010. doi:10.1016/j.humimm.2009.11.008. PMID 19931339. 
  13. ↑ 13.0 13.1 "Serum miR-146a and miR-223 as potential new biomarkers for sepsis". Biochem Biophys Res Commun 394 (1): 184–8. 2010. doi:10.1016/j.bbrc.2010.02.145. PMID 20188071. 
  14. ↑ 14.0 14.1 Lu Han; Buchan Rachel; Cook Stuart (2010). "MicroRNA-223 regulates Glut4 expression and cardiomyocyte glucose metabolism". Cardiovasc Res 86 (3): 410–420. doi:10.1093/cvr/cvq010. PMID 20080987. 
  15. ↑ 15.0 15.1 "Association of MicroRNA-223 expression with hepatic ischemia/reperfusion injury in mice". Dig Dis Sci 54 (11): 2362–6. 2009. doi:10.1007/s10620-008-0629-8. PMID 19104939. 
  16. ↑ "An evolutionarily conserved mechanism for microRNA-223 expression revealed by microRNA gene profiling". Cell 129 (3): 617–31. 2007. doi:10.1016/j.cell.2007.02.048. PMID 17482553. 
  17. ↑ "Sequence context outside the target region influences the effectiveness of miR-223 target sites in the RhoB 3'UTR". Nucleic Acids Res 38 (1): 239–52. 2010. doi:10.1093/nar/gkp870. PMID 19850724. 
  18. ↑ "MicroRNA-223 regulates cyclin E activity by modulating expression of F-box and WD-40 domain protein 7". J Biol Chem 285 (45): 34439–46. 2010. doi:10.1074/jbc.M110.152306. PMID 20826802. 
  19. ↑ 19.0 19.1 19.2 "MicroRNA 223-dependent expression of LMO2 regulates normal erythropoiesis". Haematologica 94 (4): 479–86. 2009. doi:10.3324/haematol.2008.002345. PMID 19278969. 
  20. ↑ "A minicircuitry comprised of microRNA-223 and transcription factors NFI-A and C/EBPalpha regulates human granulopoiesis". Cell 123 (5): 819–31. 2005. doi:10.1016/j.cell.2005.09.023. PMID 16325577. 
  21. ↑ "MicroRNA-223 is a key factor in osteoclast differentiation". J Cell Biochem 101 (4): 996–9. 2007. doi:10.1002/jcb.21335. PMID 17471500. 

Further reading

  • Song, L; Duan, P; Guo, P; Li, D; Li, S; Xu, Y; Zhou, Q (15 December 2012). "Downregulation of miR-223 and miR-153 mediates mechanical stretch-stimulated proliferation of venous smooth muscle cells via activation of the insulin-like growth factor-1 receptor". Archives of Biochemistry and Biophysics 528 (2): 204–11. doi:10.1016/j.abb.2012.08.015. PMID 23046980. 

External links



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