Biology:Choline transporter
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The high-affinity choline transporter (ChT) also known as solute carrier family 5 member 7 is a protein in humans that is encoded by the SLC5A7 gene.[1] It is a cell membrane transporter and carries choline into acetylcholine-synthesizing neurons.
Hemicholinium-3 is an inhibitor of the ChT and can be used to deplete acetylcholine stores, while coluracetam is an enhancer of the ChT and can increase cholinergic neurotransmission by enhancing acetylcholine synthesis.
Function
Choline is a direct precursor of acetylcholine (ACh), a neurotransmitter of the central and peripheral nervous system that regulates a variety of autonomic, cognitive, and motor functions. SLC5A7 is a Na(+)- and Cl(-)- dependent high-affinity transporter that mediates the uptake of choline for acetylcholine synthesis in cholinergic neurons.[1][2]
Mutations in the SLC5A7 gene have been associated with Distal spinal muscular atrophy with vocal cord paralysis (distal hereditary motor neuropathy type 7A).[3]
The ChT seems to be a site of action of some β-neurotoxins found in snake venoms, which disrupt peripheral cholinergic transmission by interfering with presynaptic acetylcholine synthesis. It is hypothesized that these toxins irreversibly block the ChT.[4][5]
Choline transporter in the human brain microvascular endothelial cells
Choline is a necessary reagent for the synthesis of acetylcholine in the central nervous system. Neurons get their choline by specific protein transporters known as choline transporters. In the human brain microvascular endothelial cells, two systems initiate the choline absorption. The first system is known as the Choline transporter-like protein 1, or CTL1. The second system is the Choline transporter-like protein 2, or CTL2. Those two systems are found on the plasma membrane of the brain microvascular endothelial cells. They are also found on the mitochondrial membrane. CTL2 was found to be highly expressed on the mitochondria. Meanwhile, CTL1 was mostly found on the plasma membrane of those microvascular cells.[6]
CTL2 is the main protein involved in the absorption of choline into the mitochondria for its oxidation, and CTL1 is the main protein for the choline uptake from the extracellular medium. CTL1 is a pH dependent protein. The absorption of choline through CTL1 proteins changes with the pH of the extracellular medium. When the pH of the medium is changed from 7.5 to 7.0-5.5, the rate of absorption of choline by CTL1 proteins decreases greatly. The choline uptake does not change upon the alkalinization of the extracellular medium. Moreover, it was found that the choline uptake is also influenced by the electronegativity of the plasma membrane. When the concentration of potassium ions is increased, the membrane becomes depolarized. The choline absorption decreases majorly as a result of the membrane depolarization by the potassium ions. The choline uptake was found to be only affected by the potassium ions. The sodium ions do not affect the affinity of CTL1 and CTL2 to choline.[6]
See also
References
- ↑ 1.0 1.1 "Entrez Gene: Solute carrier family 5 (choline transporter), member 7". https://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=retrieve&list_uids=60482.
- ↑ "Molecular cloning of a human, hemicholinium-3-sensitive choline transporter". Biochemical and Biophysical Research Communications 276 (3): 862–7. October 2000. doi:10.1006/bbrc.2000.3561. PMID 11027560.
- ↑ "Defective presynaptic choline transport underlies hereditary motor neuropathy". American Journal of Human Genetics 91 (6): 1103–7. December 2012. doi:10.1016/j.ajhg.2012.09.019. PMID 23141292.
- ↑ "Inhibition of high-affinity choline transport in peripheral cholinergic endings by presynaptic snake venom neurotoxins". Nature 269 (5630): 700–2. October 1977. doi:10.1038/269700a0. PMID 593330. Bibcode: 1977Natur.269..700D.
- ↑ "The action of notexin from tiger snake venom (Notechis scutatus scutatus) on acetylcholine release and compartmentation in synaptosomes from electric organ of Torpedo marmorata". Toxicon 28 (9): 1039–52. 1990. doi:10.1016/0041-0101(90)90142-T. PMID 2260102.
- ↑ 6.0 6.1 "Functional expression of choline transporter like-protein 1 (CTL1) and CTL2 in human brain microvascular endothelial cells". Neurochemistry International 93: 40–50. February 2016. doi:10.1016/j.neuint.2015.12.011. PMID 26746385.
This article incorporates text from the United States National Library of Medicine, which is in the public domain.
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