Biology:Ammonia transporter

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Ammonia transporter
Rhcg 3hd6.png
Human Rhesus C Glycoprotein. PDB 3hd6[1]
Identifiers
SymbolAmtB
PfamPF00909
InterProIPR001905
TCDB1.A.11
OPM superfamily13
OPM protein2ns1

Ammonia transporters (TC# 1.A.11) are structurally related membrane transport proteins called Amt proteins (ammonia transporters) in bacteria and plants, methylammonium/ammonium permeases (MEPs) in yeast, or Rhesus (Rh) proteins in chordates. In humans, the RhAG, RhBG, and RhCG Rhesus proteins constitute solute carrier family 42[2] whilst RhD and RhCE form the Rh blood group system. The three-dimensional structure of the ammonia transport protein AmtB from Escherichia coli has been determined by x-ray crystallography[3][4] revealing a hydrophobic ammonia channel.[5] The human RhCG ammonia transporter was found to have a similar ammonia-conducting channel structure.[1] It was proposed[citation needed] that the erythrocyte Rh complex is a heterotrimer of RhAG, RhD, and RhCE subunits in which RhD and RhCE might play roles in anchoring the ammonia-conducting RhAG subunit to the cytoskeleton. Based on reconstitution experiments, purified RhCG subunits alone can function to transport ammonia.[6] RhCG is required for normal acid excretion by the mouse kidney[7] and epididymis.[8]

Structure

The structure of the ammonia channel from E. coli,[3][4] was, at the time of its publication, the highest resolution structure of any integral membrane protein. It shows a trimer of subunits, each made up of 11 transmembrane segments (TMSs) and containing a pseudo two-fold symmetry.[9] Each monomer contains a hydrophobic ammonia conducting channel.

While prokaryotic ammonia channel proteins have an N-terminal region which acts as a signal sequence and is cleaved in the mature protein,[10] the Rhesus glycoproteins retain this as a 12th transmembrane helix in the mature protein.[1]

Substrate specificity

Most functionally characterized members of the family are ammonium uptake transporters.[11] Some, but not other Amt proteins also transport methylammonium.[12][13] Detailed phylogenetic analyses of plant homologues have been published.[14] In E. coli, NH4+, rather than NH3, may be the substrate of AmtB, but controversy still exists.[15][16][17] If NH4+ is transported, K+ possibly serves as a counter ion in an antiport process with K+, and that one histidine removes a proton off of NH4+ to yield NH3.[15]

Transport reaction

The generalized transport reaction catalyzed by members of the Amt family are suggested to be:

NH4+ (out) ⇌ NH4+ (in)

Mechanism

The X-ray structures have revealed that the pore of the Amt and Rh proteins is characterized by a hydrophobic portion about 12 Å long, in which electronic density was observed in the crystallographic study of AmtB from Escherichia coli. This electronic density was initially only observed when crystals were grown in the presence of ammonium, and was thus attributed to ammonia molecules. The Amt/Rh protein mechanism might involve the single-file diffusion of NH3 molecules. However, the pore could also be filled with water molecules. The possible presence of water molecules in the pore lumen calls for a reassessment of the notion that Amt/Rh proteins work as plain NH3 channels. Indeed, functional experiments on plant ammonium transporters and Rh proteins suggest a variety of permeation mechanisms including the passive diffusion of NH3, the antiport of NH4+/H+, the transport of NH4+, or the cotransport of NH3/H+. Lamoureux et al. discuss these mechanisms in light of functional and simulation studies on the AmtB transporter.[18]

Regulation

In E. coli the AmtB gene is expressed only under limiting nitrogen levels to yield the AmtB protein. It is co-expressed with the GlnK gene which encodes a PII protein. This protein is also trimeric and remains in the cytoplasm.[19] It is covalently modified by a U/U deuridylylated group at Y51. The hydrolyzed product, adenosine 5'-diphosphate, orients the surface of GlnK for AmtB blockade. When nitrogen levels outside the cell rise, the ammonia channel must be deactivated to prevent excessive ammonia entering the cell (where ammonia would be combined with glutamate to make glutamine, utilizing ATP and thereby depleting the cell's ATP reserves). This deactivation is achieved by deuridylylation of the GlnK protein which then binds to the cytoplasmic face of AmtB and inserts a loop into the ammonia conducting pore. At the tip of this loop is an arginine residue which sterically blocks the channel.[20]

Human ammonia transporter-related proteins

RHAG, RHBG, RHCE, RHCG, RHD

References

  1. ↑ 1.0 1.1 1.2 Gruswitz, F.; Chaudhary, S.; Ho, J. D.; Schlessinger, A.; Pezeshki, B.; Ho, C. -M.; Sali, A.; Westhoff, C. M. et al. (2010). "Function of human Rh based on structure of RhCG at 2.1 A". Proceedings of the National Academy of Sciences 107 (21): 9638–9643. doi:10.1073/pnas.1003587107. PMID 20457942. 
  2. ↑ "Non-erythroid Rh glycoproteins: a putative new family of mammalian ammonium transporters". Pflügers Archiv 447 (5): 807–12. Feb 2004. doi:10.1007/s00424-003-1142-8. PMID 12920597. 
  3. ↑ 3.0 3.1 1xqe; "Mechanism of ammonia transport by Amt/MEP/Rh: structure of AmtB at 1.35 A". Science 305 (5690): 1587–94. Sep 2004. doi:10.1126/science.1101952. PMID 15361618. 
  4. ↑ 4.0 4.1 2u7c; "The mechanism of ammonia transport based on the crystal structure of AmtB of Escherichia coli". Proceedings of the National Academy of Sciences of the United States of America 101 (49): 17090–5. Dec 2004. doi:10.1073/pnas.0406475101. PMID 15563598. Bibcode: 2004PNAS..10117090Z. 
  5. ↑ "The Amt/MEP/Rh family: structure of AmtB and the mechanism of ammonia gas conduction". Physiology 21 (6): 419–29. Dec 2006. doi:10.1152/physiol.00051.2005. PMID 17119155. 
  6. ↑ Fatouros, Dimitris, ed (2010). "Functional reconstitution into liposomes of purified human RhCG ammonia channel". PLOS ONE 5 (1): e8921. doi:10.1371/journal.pone.0008921. PMID 20126667. Bibcode: 2010PLoSO...5.8921M. 
  7. ↑ "The rhesus protein RhCG: a new perspective in ammonium transport and distal urinary acidification". Kidney International 79 (2): 154–61. Jan 2011. doi:10.1038/ki.2010.386. PMID 20927037. https://www.zora.uzh.ch/id/eprint/38490/4/The_Rhesus_proteins_RgCG_a_new_perspective_-_accepted_versionV.pdf. 
  8. ↑ "A role for Rhesus factor Rhcg in renal ammonium excretion and male fertility". Nature 456 (7220): 339–43. Nov 2008. doi:10.1038/nature07518. PMID 19020613. Bibcode: 2008Natur.456..339B. 
  9. ↑ "Electron and atomic force microscopy of the trimeric ammonium transporter AmtB". EMBO Reports 5 (12): 1153–8. Dec 2004. doi:10.1038/sj.embor.7400296. PMID 15568015. 
  10. ↑ "The ammonia channel protein AmtB from Escherichia coli is a polytopic membrane protein with a cleavable signal peptide". FEMS Microbiology Letters 258 (1): 114–20. May 2006. doi:10.1111/j.1574-6968.2006.00202.x. PMID 16630265. 
  11. ↑ Soupene, Eric; King, Natalie; Feild, Eithne; Liu, Phillip; Niyogi, Krishna K.; Huang, Cheng-Han; Kustu, Sydney (2002-05-28). "Rhesus expression in a green alga is regulated by CO(2)". Proceedings of the National Academy of Sciences of the United States of America 99 (11): 7769–7773. doi:10.1073/pnas.112225599. ISSN 0027-8424. PMID 12032358. Bibcode: 2002PNAS...99.7769S. 
  12. ↑ Musa-Aziz, Raif; Chen, Li-Ming; Pelletier, Marc F.; Boron, Walter F. (2009-03-31). "Relative CO2/NH3 selectivities of AQP1, AQP4, AQP5, AmtB, and RhAG". Proceedings of the National Academy of Sciences of the United States of America 106 (13): 5406–5411. doi:10.1073/pnas.0813231106. ISSN 1091-6490. PMID 19273840. Bibcode: 2009PNAS..106.5406M. 
  13. ↑ Andrade, Susana L. A.; Einsle, Oliver (2007-12-01). "The Amt/Mep/Rh family of ammonium transport proteins". Molecular Membrane Biology 24 (5–6): 357–365. doi:10.1080/09687680701388423. ISSN 0968-7688. PMID 17710640. 
  14. ↑ von Wittgenstein, Neil J. J. B.; Le, Cuong H.; Hawkins, Barbara J.; Ehlting, Jürgen (2014-01-01). "Evolutionary classification of ammonium, nitrate, and peptide transporters in land plants". BMC Evolutionary Biology 14: 11. doi:10.1186/1471-2148-14-11. ISSN 1471-2148. PMID 24438197. 
  15. ↑ 15.0 15.1 Fong, Rebecca N.; Kim, Kwang-Seo; Yoshihara, Corinne; Inwood, William B.; Kustu, Sydney (2007-11-20). "The W148L substitution in the Escherichia coli ammonium channel AmtB increases flux and indicates that the substrate is an ion". Proceedings of the National Academy of Sciences of the United States of America 104 (47): 18706–18711. doi:10.1073/pnas.0709267104. ISSN 1091-6490. PMID 17998534. Bibcode: 2007PNAS..10418706F. 
  16. ↑ Ishikita, Hiroshi; Knapp, Ernst-Walter (2007-02-07). "Protonation states of ammonia/ammonium in the hydrophobic pore of ammonia transporter protein AmtB". Journal of the American Chemical Society 129 (5): 1210–1215. doi:10.1021/ja066208n. ISSN 0002-7863. PMID 17263403. 
  17. ↑ Javelle, Arnaud; Lupo, Domenico; Zheng, Lei; Li, Xiao-Dan; Winkler, Fritz K.; Merrick, Mike (2006-12-22). "An unusual twin-his arrangement in the pore of ammonia channels is essential for substrate conductance". The Journal of Biological Chemistry 281 (51): 39492–39498. doi:10.1074/jbc.M608325200. ISSN 0021-9258. PMID 17040913. 
  18. ↑ Lamoureux, G.; Javelle, A.; Baday, S.; Wang, S.; Bernèche, S. (2010-09-01). "Transport mechanisms in the ammonium transporter family". Transfusion Clinique et Biologique 17 (3): 168–175. doi:10.1016/j.tracli.2010.06.004. ISSN 1953-8022. PMID 20674437. 
  19. ↑ "In vitro analysis of the Escherichia coli AmtB-GlnK complex reveals a stoichiometric interaction and sensitivity to ATP and 2-oxoglutarate". The Journal of Biological Chemistry 281 (40): 29558–67. Oct 2006. doi:10.1074/jbc.M602477200. PMID 16864585. 
  20. ↑ 2nuu; "The crystal structure of the Escherichia coli AmtB-GlnK complex reveals how GlnK regulates the ammonia channel". Proceedings of the National Academy of Sciences of the United States of America 104 (4): 1213–8. Jan 2007. doi:10.1073/pnas.0610348104. PMID 17220269. Bibcode: 2007PNAS..104.1213C. 

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