Biology:CLIC4

Short description: Protein-coding gene in the species Homo sapiens

Generic protein structure example

Chloride intracellular channel 4, also known as CLIC4,p644H1,HuH1, is a eukaryotic gene.

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the clic4 gene, is a member of the p64 family; the gene is expressed in many tissues. These channels are implicated in angiogenesis, pulmonary hypertension, cancer, and cardioprotection from ischemia-reperfusion injury. They exhibit an intracellular vesicular pattern in PANC-1 cells (pancreatic cancer cells).

Binding partners

CLIC4 binds to dynamin I, α-tubulin, β-actin, creatine kinase and two 14-3-3 isoforms.

See also

• Chloride channel

Further reading

• "Identification of a Novel Member of the Chloride Intracellular Channel Gene Family (CLIC5) That Associates with the Actin Cytoskeleton of Placental Microvilli". Mol. Biol. Cell 11 (5): 1509–21. 2000. doi:10.1091/mbc.11.5.1509. PMID 10793131. 
• "CLIC4 localizes to mitochondrial-associated membranes and mediates cardioprotection". Science Advances 8 (42): eabo1244. 2022. doi:10.1126/sciadv.abo1244. PMID 36269835. Bibcode: 2022SciA....8O1244P. 
• "CLIC4 is enriched at cell-cell junctions and colocalizes with AKAP350 at the centrosome and midbody of cultured mammalian cells". Cell Motil. Cytoskeleton 56 (3): 159–72. 2004. doi:10.1002/cm.10141. PMID 14569596. 
• "Proteomic analysis of vascular endothelial growth factor-induced endothelial cell differentiation reveals a role for chloride intracellular channel 4 (CLIC4) in tubular morphogenesis". J. Biol. Chem. 280 (51): 42397–404. 2006. doi:10.1074/jbc.M506724200. PMID 16239224. 
• "A 29 kDa intracellular chloride channel p64H1 is associated with large dense-core vesicles in rat hippocampal neurons". J. Neurosci. 19 (8): 2919–28. 1999. doi:10.1523/JNEUROSCI.19-08-02919.1999. PMID 10191309. 
• "Rat brain p64H1, expression of a new member of the p64 chloride channel protein family in endoplasmic reticulum". J. Biol. Chem. 272 (38): 23880–6. 1997. doi:10.1074/jbc.272.38.23880. PMID 9295337. 
• Edwards JC (1999). "A novel p64-related Cl⁻ channel: subcellular distribution and nephron segment-specific expression". Am. J. Physiol. 276 (3 Pt 2): F398–408. doi:10.1152/ajprenal.1999.276.3.F398. PMID 10070163. 
• "Molecular cloning and characterization of a mitogen-activated protein kinase-associated intracellular chloride channel". J. Biol. Chem. 274 (3): 1621–7. 1999. doi:10.1074/jbc.274.3.1621. PMID 9880541. 
• "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. 2004. doi:10.1101/gr.2596504. PMID 15489334. 
• "DNA Cloning Using In Vitro Site-Specific Recombination". Genome Res. 10 (11): 1788–95. 2001. doi:10.1101/gr.143000. PMID 11076863. 
• "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. 2004. doi:10.1038/ng1285. PMID 14702039. 
• "Differential Expression of a Chloride Intracellular Channel Gene, CLIC4, in Transforming Growth Factor-β1-Mediated Conversion of Fibroblasts to Myofibroblasts". Am. J. Pathol. 161 (2): 471–80. 2002. doi:10.1016/S0002-9440(10)64203-4. PMID 12163372. 
• "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. 2005. doi:10.1038/nature04209. PMID 16189514. Bibcode: 2005Natur.437.1173R. 
• "Quantitative proteomic analysis of myc-induced apoptosis: a direct role for Myc induction of the mitochondrial chloride ion channel, mtCLIC/CLIC4". J. Biol. Chem. 281 (5): 2750–6. 2006. doi:10.1074/jbc.M509349200. PMID 16316993. 
• "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Rep. 1 (3): 287–92. 2001. doi:10.1093/embo-reports/kvd058. PMID 11256614. 
• "CLIC4 (p64H1) and its putative transmembrane domain form poorly selective, redox-regulated ion channels". Mol. Membr. Biol. 24 (1): 41–52. 2007. doi:10.1080/09687860600927907. PMID 17453412. 
• "Functional reconstitution of mammalian 'chloride intracellular channels' CLIC1, CLIC4 and CLIC5 reveals differential regulation by cytoskeletal actin". FEBS J. 274 (24): 6306–6316. 2007. doi:10.1111/j.1742-4658.2007.06145.x. PMID 18028448. 
• Singh H (2010). "Two decades with dimorphic Chloride Intracellular Channels (CLICs)". FEBS Letters 584 (10): 2112–21. doi:10.1016/j.febslet.2010.03.013. PMID 20226783. 
• "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. 2003. doi:10.1073/pnas.242603899. PMID 12477932. Bibcode: 2002PNAS...9916899M. 
• "Chloride intracellular channel protein CLIC4 (p64H1) binds directly to brain dynamin I in a complex containing actin, tubulin and 14-3-3 isoforms". Biochem. J. 359 (Pt 1): 55–64. 2003. doi:10.1042/0264-6021:3590055. PMID 11563969. 
• "Molecular cloning and expression of a chloride ion channel of cell nuclei". J. Biol. Chem. 272 (19): 12575–82. 1997. doi:10.1074/jbc.272.19.12575. PMID 9139710. 
• "Identification of serum-inducible genes: different patterns of gene regulation during G0-->S and G1-->S progression". J. Cell Sci. 107 (1): 227–39. 1994. doi:10.1242/jcs.107.1.227. PMID 8175911. 
• "From ORFeome to Biology: A Functional Genomics Pipeline". Genome Res. 14 (10B): 2136–44. 2004. doi:10.1101/gr.2576704. PMID 15489336. 
• "Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs". Genome Res. 11 (3): 422–35. 2001. doi:10.1101/gr.GR1547R. PMID 11230166. 

External links

• CLIC4+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH)
• Human CLIC4 genome location and CLIC4 gene details page in the UCSC Genome Browser.

This article incorporates text from the United States National Library of Medicine, which is in the public domain.