Biology:TRPM8

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Short description: Protein-coding gene in the species Homo sapiens


A representation of the 3D structure of the protein myoglobin showing turquoise α-helices.
Generic protein structure example

Transient receptor potential cation channel subfamily M (melastatin) member 8 (TRPM8), also known as the cold and menthol receptor 1 (CMR1), is a protein that in humans is encoded by the TRPM8 gene.[1][2] The TRPM8 channel is the primary molecular transducer of cold somatosensation in humans.[1][3] In addition, mints can desensitize a region through the activation of TRPM8 receptors (the 'cold'/menthol receptor).[4]

Structure

The TRPM8 channel is a homotetramer, composed of four identical subunits with a transmembrane domain with six helices (S1–6). The first four, S1–4, act as the voltage sensor and allow binding of menthol, icilin and similar channel agonists. S5 and S6 and a connecting loop, also part of the structure, make up the pore, a non-selective cation channel which consists of a highly conserved hydrophobic region. A range of diverse components are required for the high level of specificity in response to cold and menthol stimuli which eventually lead to ion flow through the protein channel.[5][6]

Function

TRPM8 is an ion channel: upon activation, it allows the entry of Na+ and Ca2+ ions into the cell, which leads to depolarization and the generation of an action potential. The signal is conducted from primary afferents (type C- and A-delta) eventually leading to the sensation of cold and cold pain.[1]

The TRPM8 protein is expressed in sensory neurons, and it is activated by cold temperatures and cooling agents, such as menthol and icilin whereas WS-12 and CPS-369 are the most selective agonists of TRPM8.[7][8]

TRPM8 is also expressed in the prostate, lungs, and bladder where its function is not well understood.

Role in the nervous system

The transient receptor potential channel (TRP) superfamily, which includes the menthol (TRPM8) and capsaicin receptors (TRPV1), serve a variety of functions in the peripheral and central nervous systems. In the peripheral nervous system, TRPs respond to stimuli from temperature, pressure, inflammatory agents, and receptor activation. Central nervous system roles of the receptors include neurite outgrowth, receptor signaling, and excitotoxic cell death resulting from noxious stimuli.[9]

McKemy et al., 2002 provided some of the first evidence for existence of a cold-activated receptor throughout the mammalian somatosensory system.[1] Using calcium imaging and patch clamp based approaches, they showed a response in dorsal root ganglion (DRG) neurons that exposure to cold, 20 °C or cooler, lead to a response in calcium influx. This receptor was shown to respond to both cold temperatures, menthol, and similar now-known agonists of the TRPM8 receptor. It works in conjunction with the TRPV1 receptor to maintain a feasible threshold temperature range in which our cells are comfortable and our perception of these stimuli occurs at the spinal cord and brain, which integrate signals from different fibers of varying sensitivity to temperature. Application of menthol to skin or mucus membranes results directly in membrane depolarization, followed by calcium influx via voltage-dependent calcium channels, providing evidence for the role of TRPM8 and other TRP receptors to mediate our sensory interaction with the environment in response to cold in the same way as in response to menthol.[10]

Properties

pH-sensitivity

In contrast to the TRPV1 (capsaicin) receptor, which is potentiated by low pH, acidic conditions were shown to inhibit the TRPM8 Ca2+ response to menthol and icilin (an agonist of the menthol receptor). It is hypothesized the TRPV1 and TRPM8 receptors act together in response to inflammatory conditions: TRPV1, by proton action, increases the burning sensation of pain, while the acidity inhibits TRPM8 to block the more pleasant sensation of coolness in more dire instances of pain.[11]

Sensitization

Numerous studies have been published investigating the effect of L-menthol application as a model for TRPM8-sensitization.[1][12] The primary consensus finding is that TRPM8 sensitization increases the sensation of cold pain, also known as cold hyperalgesia.[1] An experiment was done in a double-blind two-way crossover study by applying 40% L-menthol to the forearm, using ethanol as a control. Activation of the TRPM8-receptor channel (the primary menthol receptor channel) resulted in increased sensitization to the menthol stimulus. To investigate the mechanisms of this sensitization, Wasner et al., 2004, performed A fiber conduction blockade of the superficial radial nerve in another group of subjects. This ended up reducing the menthol-induced sensation of cold and hyperalgesia because blocking A fiber conduction resulted in inhibition of a class of group C nerve fiber nociceptors needed to transduce the sensation of pain. They concluded menthol sensitizes cold-sensitive peripheral C nociceptors and activates cold-specific A delta fibers.[1][3][13]

Desensitization

As is common in response to many other sensory stimuli, much experimental evidence exists for the desensitization of human response of TRPM8 receptors to menthol.[1] Testing involving administration of menthol and nicotine-containing cigarettes non-smokers, which induced what they classified as an irritant response, after initial sensitization, showed a declining response in subjects over time, lending itself to the incidence of desensitization. Ethanol, with similar irritant and desensitization properties, was used as a control for nicotine, to distinguish it from menthol-induced response. The menthol receptor was seen to sensitize or desensitize based on cellular conditions, and menthol produces increased activity in Ca2+-voltage gated channels that is not seen in ethanol, cyclohexanol and other irritant controls, suggestive of a specific molecular receptor. Dessirier et al., 2001, also claim the cross-desensitization of menthol receptors can occur by unknown molecular mechanisms, though they hypothesize the importance of Ca2+ in reducing cell excitability in a way similar to that in the capsaicin receptor.[14]

Mutagenesis of protein kinase C phosphorylation sites in TRPM8 (wild type serines and threonines replaced by alanine in mutants) reduces the desensitizing response.[15]

Caryophyllene inhibits TRPM8, which helps mammals to improve cold tolerance at low ambient temperatures.[16]

Cross-desensitization

Cliff et al., 1994, performed a study to discover more about the properties of the menthol receptor and whether menthol had the ability to cross-desensitize with other chemical irritant receptors. Capsaicin was known to cross-desensitize with other irritant agonists, where the same information was not known about menthol. The study involved subjects swishing either menthol or capsaicin for an extended time at regular intervals. There were three significant conclusions about cross-desensitizing: 1) Both chemicals self-desensitize, 2) menthol receptors can desensitize in response to capsaicin, and, most novelly, 3) capsaicin receptors are sensitized in response to menthol.[17]

Ligands

Agonists

In a search for compounds that activated the TRPM8 cold receptor, compounds that produce a cooling-sensation were sought out from the fragrance industries. Of 70 relevant compounds, the following 10 produced the associated [Ca2+]-increase response in mTRPM8-transfected HEK293 cells used to identify agonists. Experimentally identified and commonly utilized agonists of the menthol receptor include linalool, geraniol, hydroxy-citronellal, icilin, WS-12, Frescolat MGA, Frescolat ML, PMD 38, Coolact P, M8-Ag and Cooling Agent 10.[11][12] Traditionally used agonists include menthol[18] and borneol.[19]

Antagonists

BCTC, thio-BCTC, capsazepine and M8-An[20] were identified as antagonists of the TRPM8 receptor. These antagonists physically block the receptor for cold and menthol, by binding to the S1-S4 voltage-sensing domain, preventing response.[11]

Clinical significance

Cold-patches have traditionally been used to induce analgesia or relief in pain which is caused as result of traumatic injuries.[25] The underlying mechanism of cold-induced analgesia remained obscure until the discovery of TRPM8.

One research group has reported that TRPM8 is activated by chemical cooling agents (such as menthol) or when ambient temperatures drop below approximately 26 °C, suggesting that it mediates the detection of cold thermal stimuli by primary afferent sensory neurons of afferent nerve fibers.[26]

Three independent research groups have reported that mice lacking functional TRPM8 gene expression are severely impaired in their ability to detect cold temperatures.[27] Remarkably, these animals are deficient in many diverse aspects of cold signaling, including cool and noxious cold perception, injury-evoked sensitization to cold, and cooling-induced analgesia. These animals provide a great deal of insight into the molecular signaling pathways that participate in the detection of cold and painful stimuli. Many research groups, both in universities and pharmaceutical companies, are now actively involved in looking for selective TRPM8 ligands to be used as new generation of neuropathic analgesic drugs.[12][20]

Low concentrations of TRPM8 agonists such as menthol (or icilin) found to be antihyperalgesic in certain conditions,[28] whereas high concentrations of menthol caused both cold and mechanical hyperalgesia in healthy volunteers.[13]

TRPM8 knockout mice not only indicated that TRPM8 is required for cold sensation but also revealed that TRPM8 mediates both cold and mechanical allodynia in rodent models of neuropathic pain.[29] Furthermore, recently it was shown that TRPM8 antagonists are effective in reversing established pain in neuropathic and visceral pain models.[30][20]

TRPM8 upregulation in bladder tissues correlates with pain in patients with painful bladder syndromes.[31] Furthermore, TRPM8 is upregulated in many prostate cancer cell lines and Dendreon/Genentech are pursuing an agonist approach to induce apoptosis and prostate cancer cell death.[32]

Role in cancer

TRPM8 channels may be a target for treating prostate cancer. TRPM8 is an androgen dependent Ca2+ channel necessary for prostate cancer cells to survive and grow. Immunofluorescence showed expression of the TRPM8 protein in the ER and plasma membrane of the androgen-responsive LNCaP cell line. TRPM8 was expressed in androgen-insensitive cells, but it was not shown to be needed for their survival. By knockout of TRPM8 with siRNAs targeting TRPM8 mRNAs, the necessity of the TRPM8 receptor was shown in the androgen-dependent cancer cells. This has useful implications in terms of gene therapy, as there are so few treatment options for men with prostate cancer. As an androgen-regulated protein whose function is lost as cancer develops in cells, the TRPM8 protein seems to be especially critical in regulating calcium levels and has recently been proposed as the focus of new drugs used to treat prostate cancer.[33]

See also

References

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 "A review of topical high-concentration L-menthol as a translational model of cold allodynia and hyperalgesia". European Journal of Pain 18 (3): 315–25. March 2014. doi:10.1002/j.1532-2149.2013.00380.x. PMID 23963768. 
  2. ↑ "International Union of Pharmacology. XLIX. Nomenclature and structure-function relationships of transient receptor potential channels". Pharmacological Reviews 57 (4): 427–50. December 2005. doi:10.1124/pr.57.4.6. PMID 16382100. 
  3. ↑ 3.0 3.1 "Somatosensory and vasomotor manifestations of individual and combined stimulation of TRPM8 and TRPA1 using topical L-menthol and trans-cinnamaldehyde in healthy volunteers". European Journal of Pain 18 (9): 1333–42. October 2014. doi:10.1002/j.1532-2149.2014.494.x. PMID 24664788. 
  4. ↑ "Mu and kappa opioid receptor agonists antagonize icilin-induced wet-dog shaking in rats". European Journal of Pharmacology 547 (1–3): 101–5. October 2006. doi:10.1016/j.ejphar.2006.07.026. PMID 16945367. 
  5. ↑ "Comparative modeling of the quaternary structure for the human TRPM8 channel and analysis of its binding features". Biochimica et Biophysica Acta (BBA) - Biomembranes 1788 (5): 973–82. May 2009. doi:10.1016/j.bbamem.2009.02.007. PMID 19230823. 
  6. ↑ "Structural insights into TRPM8 inhibition and desensitization". Science 365 (6460): 1434–1440. September 2019. doi:10.1126/science.aax6672. PMID 31488702. Bibcode: 2019Sci...365.1434D. 
  7. ↑ Sherkheli M.A. (2007). "Selective TRPM8 agonists: a novel group of neurophathic analgesics". FEBS Journal 274 (s1): 232. doi:10.1111/j.0014-2956.2007.05861_4.x. 
  8. ↑ "Menthol derivative WS-12 selectively activates transient receptor potential melastatin-8 (TRPM8) ion channels". Pakistan Journal of Pharmaceutical Sciences 21 (4): 370–8. October 2008. PMID 18930858. http://www.pjps.pk/. 
  9. ↑ "TRP ion channels in the nervous system". Current Opinion in Neurobiology 14 (3): 362–9. June 2004. doi:10.1016/j.conb.2004.05.003. PMID 15194117. 
  10. ↑ "Identification of a cold receptor reveals a general role for TRP channels in thermosensation". Nature 416 (6876): 52–8. March 2002. doi:10.1038/nature719. PMID 11882888. Bibcode: 2002Natur.416...52M. 
  11. ↑ 11.0 11.1 11.2 "Characterization of the mouse cold-menthol receptor TRPM8 and vanilloid receptor type-1 VR1 using a fluorometric imaging plate reader (FLIPR) assay". British Journal of Pharmacology 141 (4): 737–45. February 2004. doi:10.1038/sj.bjp.0705652. PMID 14757700. 
  12. ↑ 12.0 12.1 12.2 "Anti-hyperalgesic effects of a novel TRPM8 agonist in neuropathic rats: a comparison with topical menthol". Pain 155 (10): 2097–107. October 2014. doi:10.1016/j.pain.2014.07.022. PMID 25083927. 
  13. ↑ 13.0 13.1 "Topical menthol--a human model for cold pain by activation and sensitization of C nociceptors". Brain 127 (Pt 5): 1159–71. May 2004. doi:10.1093/brain/awh134. PMID 14985268. 
  14. ↑ "Oral irritant properties of menthol: sensitizing and desensitizing effects of repeated application and cross-desensitization to nicotine". Physiology & Behavior 73 (1–2): 25–36. May 2001. doi:10.1016/S0031-9384(01)00431-0. PMID 11399291. 
  15. ↑ "Ca2+-dependent PKC activation mediates menthol-induced desensitization of transient receptor potential M8". Neuroscience Letters 397 (1–2): 140–4. 2006. doi:10.1016/j.neulet.2005.12.005. PMID 16380208. 
  16. ↑ "Why wild giant pandas frequently roll in horse manure". Proceedings of the National Academy of Sciences of the United States of America 117 (51): 32493–32498. December 2020. doi:10.1073/pnas.2004640117. PMID 33288697. Bibcode: 2020PNAS..11732493Z. 
  17. ↑ "Sensitization and desensitization to capsaicin and menthol in the oral cavity: interactions and individual differences". Physiology & Behavior 59 (3): 487–94. March 1996. doi:10.1016/0031-9384(95)02089-6. PMID 8700951. 
  18. ↑ "Menthol and related cooling compounds". The Journal of Pharmacy and Pharmacology 46 (8): 618–30. August 1994. doi:10.1111/j.2042-7158.1994.tb03871.x. PMID 7529306. 
  19. ↑ "Borneol Is a TRPM8 Agonist that Increases Ocular Surface Wetness". PLOS ONE 11 (7): e0158868. 2016. doi:10.1371/journal.pone.0158868. PMID 27448228. Bibcode: 2016PLoSO..1158868C. 
  20. ↑ 20.0 20.1 20.2 "Novel TRPM8 antagonist attenuates cold hypersensitivity after peripheral nerve injury in rats". The Journal of Pharmacology and Experimental Therapeutics 349 (1): 47–55. April 2014. doi:10.1124/jpet.113.211243. PMID 24472724. 
  21. ↑ "5-benzyloxytryptamine as an antagonist of TRPM8". Bioorganic & Medicinal Chemistry Letters 20 (23): 7076–9. December 2010. doi:10.1016/j.bmcl.2010.09.099. PMID 20965726. 
  22. ↑ 22.0 22.1 "Regulation of transient receptor potential channels of melastatin type 8 (TRPM8): effect of cAMP, cannabinoid CB(1) receptors and endovanilloids". Experimental Cell Research 313 (9): 1911–1920. May 2007. doi:10.1016/j.yexcr.2007.01.008. PMID 17428469. 
  23. ↑ 23.0 23.1 23.2 23.3 23.4 "Plant-derived cannabinoids modulate the activity of transient receptor potential channels of ankyrin type-1 and melastatin type-8". The Journal of Pharmacology and Experimental Therapeutics 325 (3): 1007–1015. June 2008. doi:10.1124/jpet.107.134809. PMID 18354058. 
  24. ↑ 24.0 24.1 24.2 24.3 24.4 24.5 24.6 "Effects of cannabinoids and cannabinoid-enriched Cannabis extracts on TRP channels and endocannabinoid metabolic enzymes". British Journal of Pharmacology 163 (7): 1479–1494. August 2011. doi:10.1111/j.1476-5381.2010.01166.x. PMID 21175579. 
  25. ↑ "Genes, molecules and patients--emerging topics to guide clinical pain research". European Journal of Pharmacology 716 (1–3): 188–202. September 2013. doi:10.1016/j.ejphar.2013.01.069. PMID 23500200. 
  26. ↑ "The menthol receptor TRPM8 is the principal detector of environmental cold". Nature 448 (7150): 204–8. July 2007. doi:10.1038/nature05910. PMID 17538622. Bibcode: 2007Natur.448..204B. 
  27. ↑ "Mice left out in the cold: commentary on the phenotype of TRPM8-nulls". Molecular Pain 3 (1): 23. August 2007. doi:10.1186/1744-8069-3-23. PMID 17705869. 
  28. ↑ "Analgesia mediated by the TRPM8 cold receptor in chronic neuropathic pain". Current Biology 16 (16): 1591–605. August 2006. doi:10.1016/j.cub.2006.07.061. PMID 16920620. 
  29. ↑ "Attenuated cold sensitivity in TRPM8 null mice". Neuron 54 (3): 379–86. May 2007. doi:10.1016/j.neuron.2007.04.017. PMID 17481392. 
  30. ↑ "AMTB, a TRPM8 channel blocker: evidence in rats for activity in overactive bladder and painful bladder syndrome". American Journal of Physiology. Renal Physiology 295 (3): F803-10. September 2008. doi:10.1152/ajprenal.90269.2008. PMID 18562636. 
  31. ↑ "Cool and menthol receptor TRPM8 in human urinary bladder disorders and clinical correlations". BMC Urology 6: 6. March 2006. doi:10.1186/1471-2490-6-6. PMID 16519806. 
  32. ↑ "Dendreon: Targeting Cancer, Transforming Lives". Dendreon Corporation. 2005-09-21. http://investor.dendreon.com/ReleaseDetail.cfm?ReleaseID=174120&Header=News. 
  33. ↑ "Evidence that TRPM8 is an androgen-dependent Ca2+ channel required for the survival of prostate cancer cells". Cancer Research 64 (22): 8365–73. November 2004. doi:10.1158/0008-5472.CAN-04-2146. PMID 15548706. 

Further reading

External links

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