Biology:VDAC1
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Voltage-dependent anion-selective channel 1 (VDAC-1) is a beta barrel protein that in humans is encoded by the VDAC1 gene located on chromosome 5.[1][2] It forms an ion channel in the outer mitochondrial membrane (OMM) and also the outer cell membrane. In the OMM, it allows ATP to diffuse out of the mitochondria into the cytoplasm. In the cell membrane, it is involved in volume regulation. Within all eukaryotic cells, mitochondria are responsible for synthesis of ATP among other metabolite needed for cell survival. VDAC1 therefore allows for communication between the mitochondrion and the cell mediating the balance between cell metabolism and cell death. Besides metabolic permeation, VDAC1 also acts as a scaffold for proteins such as hexokinase that can in turn regulate metabolism.[3]
This protein is a voltage-dependent anion channel and shares high structural homology with the other VDAC isoforms (VDAC2 and VDAC3), which are involved in the regulation of cell metabolism, mitochondrial apoptosis, and spermatogenesis.[4][5][6][7] Over expression and misregulation of this pore could lead to apoptosis in the cell leading to a variety of diseases within the body. In particular, since VDAC1 is the major calcium ion transport channel, its dysfunction is implicated in cancer, Parkinson's (PD), and Alzheimer's disease.[8][9][10] In addition, recent studies have shown that an over expression within the VDAC1 protein is linked to Type 2 Diabetes. Lund University released a study that demonstrated the effects of blocking VDAC1 over expression can prevent the spread of Type 2 Diabetes.[11]
Structure
The three VDAC isoforms (VDAC1, VDAC2, and VDAC3) have highly conserved DNA sequences as well as 3D structures forming a wide β-barrel structure, inside of which the alpha helical N-terminal segment resides to partially close the pore.[12] VDAC1's structure was solved by 3 independent labs by x-ray crystallography, Nuclear Magnetic Resonance (NMR) spectroscopy, or a combination of both. Two of these structural studies were used to determine human VDAC1 (hVDAC1) structure while X-ray crystallography was used to solve murine VDAC1 (mVDAC1) structure that differs from hVDAC1 by only two residues.[13][14][15] These determined structures aligned with earlier circular dichroism studies that predicted the presence of alpha helix and β-strand domains.[16]
Structural analysis of mVDAC1's structure showed a barrel-like channel composed of 19 amphipathic β-strands, with the N-terminus and C-terminus both facing towards the inter membrane space of the mitochondrion.[17][18] β-strands are connected via loops and are arranged in an anti-parallel pattern with the exception of β-strands 1 and 19 which are parallel.[15] The pore has a height of 40 Ẳ, spans a distance of 27 Ẳ by 20 Ẳ at the openings and tapers down to 20 Ẳ by 14 Ẳ at the N-terminal α-helix segment in the open state.[19] The closed state conformation has yet to be isolated and determined. Additionally, the N-terminus has an alpha helical segment that is held to the inside wall of the pore by hydrophobic interactions with residues on β-sheets 8-18.[15] This N-terminus can serve as a scaffold for the movement of ions or attachment of proteins. One such example is seen as it is the docking site for HK1 binding.[3] A significant residue to point out is the glutamate located at the 73rd residue on the amino acid chain (E73). This residue is found in VDAC1 and VDAC2 but not VDAC3. The side chain of this charged residue points into the phospholipid bilayer which would normally cause repulsive forces to occur. E73 however, has been implicated in VDAC1 function and interaction.[20]
Function
VDAC1 belongs to the mitochondrial porin family and is expected to share similar biological functions to the other VDAC isoforms.[21] Of the three isoforms, VDAC1 is the main calcium ion transport channel and the most abundantly transcribed.[9][22] VDAC1 is involved in cell metabolism by transporting ATP and other small metabolites across the outer mitochondrial membrane (OMM) allowing regulation of the TCA cycle and, by extension, reactive oxygen species (ROS) production.[8] In yeast cells, ROS accumulates in response to oxidative stress, which results in impaired mitochondrial function and a “petite” phenotype. However, petite yeast cells exhibit a longer lifespan than wild-type cells and indicate a protective function by VDAC1 in similar circumstances, such as aging.[3][22]
Voltage gating
VDAC1 allows for the conductance of molecules into and out of the mitochondrion. Its permeability is dependent on VDAC1's conformational state which is determined by voltage. At low voltage (10mV), the pore is in an "open" state where the channel is weakly anion selective and allows for a greater flux of metabolites. Because of the large pore size, metabolic gating under saturated ATP conditions reveal a transport of 2,000,000 ATP/second and a transport of 10,000 ATP under physiological conditions.[23] At a higher voltage in the positive or negative direction (>30mV), the pore is in a "closed" state and is weakly cation selective allowing for less metabolites to be transported.[15] The flux of metabolites can be seen as negligible. This change in states is mediated by a conformational change in the protein that has yet to be discovered. Since the alpha helical N-terminus segment is located in the center of the pore, it is ideally situated for metabolic gating. This lead researchers to believe that the Alpha helix was a key contributor to determining the conformational states. However, more recent studies have shown the N-terminal is unnecessary for proper voltage gating and therefore suggest the flexible beta barrel as the mechanism of conformational change.[19]
Oligomerization
Atomic Force Microscopy (AFM) revealed the presence of VDAC1 monomers as well as dimers and larger oligomers showcasing the interaction of the pore with itself, however, dimers are more frequent.[24] hVDAC1 in particular has been shown to arrange in parallel dimers leading to increased permeability of the pore.[13] The glutamate located at the 73rd position on VDAC1 has also been shown to play a role in oligomerization when in the presence of calcium.[20] VDACs can also oligomerize to form part of the mitochondrial permeability transition pore (MPTP) and, thus, facilitate cytochrome C release, leading to apoptosis. VDACs have also been observed to interact with pro- or antiapoptotic proteins, such as Bcl-2 family proteins and kinases, and so may contribute to apoptosis independently from the MPTP.[21]
Clinical significance
The voltage dependent anion channels all function in ion and metabolite transport although their physiological roles are different. Because of their role, dysfunction of the channels can lead to various diseases. VDAC1 has been implicated in cancer through its interactions with the antiapoptotic family of proteins, Bcl-2 proteins, particularly Bcl-xl, and Mcl-1, which are overexpressed during cancer. These two Bcl-2 proteins interact with VDAC1 to regulate calcium ion transport across the OMM and, ultimately, ROS production. While high levels of ROS induce cell death, non-lethal levels interfere with signal transduction pathways that can then promote cell proliferation, migration, and invasion in cancer cells.[8] Moreover, VDAC1 overexpression has been associated with increased apoptotic response and anti-cancer drugs and treatment efficacy, further supporting VDAC1 as a therapeutic target for cancer treatment.[8][25]
VDAC1's function in calcium ion transport has also been linked to neurodegenerative diseases. In PD, VDAC1 increases calcium ion levels within the mitochondria, resulting in increased mitochondrial permeability, disrupted mitochondrial membrane potential, elevated ROS production, cell death, and neuronal degeneration.[9] VDAC1 has been shown to interact with Amyloid β (Aβ) leading to increased conductance of the channel and eventually apoptosis of the cell.[10]
Interactions
VDAC1 acts as a scaffold for many proteins as well as allows for the flux of ions and metabolites through interactions within the pore.
A major metabolite that moves through this channel is ATP. A low affinity binding site used for fast transport of this molecule was discovered by the Markov state modeling approach. It was shown that ATP binds to multiple basic residues within the pore sequentially, in essence moving through the channel.[26]
VDAC1 has also been shown to interact with:
- BCL2-like 1,[27][28][29][30]
- Bcl-2-associated X protein,[27][28]
- DYNLT3,[31]
- Gelsolin,[32]
- PRKCE,[33]
- HK1[3]
- Parkin[34]
- eNOS[5]
- Mcl-1[8]
- HK[25]
- ATP[35]
- GSK-3[36]
See also
References
- ↑ "Human genes encoding the voltage-dependent anion channel (VDAC) of the outer mitochondrial membrane: mapping and identification of two new isoforms". Genomics 20 (1): 62–67. March 1994. doi:10.1006/geno.1994.1127. PMID 7517385.
- ↑ "Entrez Gene: VDAC1 voltage-dependent anion channel 1". https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=7416.
- ↑ 3.0 3.1 3.2 3.3 "Swapping of the N-terminus of VDAC1 with VDAC3 restores full activity of the channel and confers anti-aging features to the cell". FEBS Letters 584 (13): 2837–2844. July 2010. doi:10.1016/j.febslet.2010.04.066. PMID 20434446.
- ↑ "Voltage-dependent anion channel 2 modulates resting Ca²+ sparks, but not action potential-induced Ca²+ signaling in cardiac myocytes". Cell Calcium 49 (2): 136–143. February 2011. doi:10.1016/j.ceca.2010.12.004. PMID 21241999.
- ↑ 5.0 5.1 "Voltage-dependent anion channel-2 interaction with nitric oxide synthase enhances pulmonary artery endothelial cell nitric oxide production". American Journal of Respiratory Cell and Molecular Biology 47 (5): 669–678. November 2012. doi:10.1165/rcmb.2011-0436OC. PMID 22842492.
- ↑ "VDAC2 inhibits BAK activation and mitochondrial apoptosis". Science 301 (5632): 513–517. July 2003. doi:10.1126/science.1083995. PMID 12881569. Bibcode: 2003Sci...301..513C.
- ↑ "Critical role for voltage-dependent anion channel 2 in infectious bursal disease virus-induced apoptosis in host cells via interaction with VP5". Journal of Virology 86 (3): 1328–1338. February 2012. doi:10.1128/JVI.06104-11. PMID 22114330.
- ↑ 8.0 8.1 8.2 8.3 8.4 "Mcl-1 promotes lung cancer cell migration by directly interacting with VDAC to increase mitochondrial Ca2+ uptake and reactive oxygen species generation". Cell Death & Disease 5 (10): e1482. October 2014. doi:10.1038/cddis.2014.419. PMID 25341036.
- ↑ 9.0 9.1 9.2 "Abnormal alpha-synuclein reduces nigral voltage-dependent anion channel 1 in sporadic and experimental Parkinson's disease". Neurobiology of Disease 69: 1–14. September 2014. doi:10.1016/j.nbd.2014.05.003. PMID 24825319.
- ↑ 10.0 10.1 "The Voltage-dependent Anion Channel 1 Mediates Amyloid β Toxicity and Represents a Potential Target for Alzheimer Disease Therapy". The Journal of Biological Chemistry 290 (52): 30670–30683. December 2015. doi:10.1074/jbc.M115.691493. PMID 26542804.
- ↑ "Preserving Insulin Secretion in Diabetes by Inhibiting VDAC1 Overexpression and Surface Translocation in β Cells". Cell Metabolism 29 (1): 64–77.e6. January 2019. doi:10.1016/j.cmet.2018.09.008. PMID 30293774.
- ↑ "Charged residues distribution modulates selectivity of the open state of human isoforms of the voltage dependent anion-selective channel". PLOS ONE 9 (8): e103879. 2014. doi:10.1371/journal.pone.0103879. PMID 25084457. Bibcode: 2014PLoSO...9j3879A.
- ↑ 13.0 13.1 "Structure of the human voltage-dependent anion channel". Proceedings of the National Academy of Sciences of the United States of America 105 (40): 15370–15375. October 2008. doi:10.1073/pnas.0808115105. PMID 18832158. Bibcode: 2008PNAS..10515370B.
- ↑ "Solution structure of the integral human membrane protein VDAC-1 in detergent micelles". Science 321 (5893): 1206–1210. August 2008. doi:10.1126/science.1161302. PMID 18755977. Bibcode: 2008Sci...321.1206H.
- ↑ 15.0 15.1 15.2 15.3 "The crystal structure of mouse VDAC1 at 2.3 A resolution reveals mechanistic insights into metabolite gating". Proceedings of the National Academy of Sciences of the United States of America 105 (46): 17742–17747. November 2008. doi:10.1073/pnas.0809634105. PMID 18988731. Bibcode: 2008PNAS..10517742U.
- ↑ "Correct folding of the beta-barrel of the human membrane protein VDAC requires a lipid bilayer". Journal of Molecular Biology 368 (1): 66–78. April 2007. doi:10.1016/j.jmb.2007.01.066. PMID 17336328. http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-42582.
- ↑ "The role of VDAC in cell death: friend or foe?". Biochimica et Biophysica Acta (BBA) - Biomembranes 1818 (6): 1444–1450. June 2012. doi:10.1016/j.bbamem.2011.10.025. PMID 22062421.
- ↑ "The voltage-dependent anion selective channel 1 (VDAC1) topography in the mitochondrial outer membrane as detected in intact cell". PLOS ONE 8 (12): e81522. December 2013. doi:10.1371/journal.pone.0081522. PMID 24324700. Bibcode: 2013PLoSO...881522T.
- ↑ 19.0 19.1 "Affixing N-terminal α-helix to the wall of the voltage-dependent anion channel does not prevent its voltage gating". The Journal of Biological Chemistry 287 (14): 11437–11445. March 2012. doi:10.1074/jbc.M111.314229. PMID 22275367.
- ↑ 20.0 20.1 "The role of calcium in VDAC1 oligomerization and mitochondria-mediated apoptosis". Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1833 (7): 1745–1754. July 2013. doi:10.1016/j.bbamcr.2013.03.017. PMID 23542128.
- ↑ 21.0 21.1 "Identification of the hypoxia-inducible factor 1 alpha-responsive HGTD-P gene as a mediator in the mitochondrial apoptotic pathway". Molecular and Cellular Biology 24 (9): 3918–3927. May 2004. doi:10.1128/mcb.24.9.3918-3927.2004. PMID 15082785.
- ↑ 22.0 22.1 "Characterization of human VDAC isoforms: a peculiar function for VDAC3?". Biochimica et Biophysica Acta (BBA) - Bioenergetics 1797 (6–7): 1268–1275. 2010. doi:10.1016/j.bbabio.2010.01.031. PMID 20138821.
- ↑ "VDAC channels mediate and gate the flow of ATP: implications for the regulation of mitochondrial function". Biophysical Journal 72 (5): 1954–1962. May 1997. doi:10.1016/s0006-3495(97)78841-6. PMID 9129800. Bibcode: 1997BpJ....72.1954R.
- ↑ "The supramolecular assemblies of voltage-dependent anion channels in the native membrane". Journal of Molecular Biology 370 (2): 246–255. July 2007. doi:10.1016/j.jmb.2007.04.073. PMID 17524423.
- ↑ 25.0 25.1 "Ca(2+)-mediated regulation of VDAC1 expression levels is associated with cell death induction". Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1843 (10): 2270–2281. October 2014. doi:10.1016/j.bbamcr.2014.03.021. PMID 24704533.
- ↑ "Structure-guided simulations illuminate the mechanism of ATP transport through VDAC1". Nature Structural & Molecular Biology 21 (7): 626–632. July 2014. doi:10.1038/nsmb.2841. PMID 24908397.
- ↑ 27.0 27.1 "Specific cleavage of Mcl-1 by caspase-3 in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in Jurkat leukemia T cells". The Journal of Biological Chemistry 280 (11): 10491–10500. March 2005. doi:10.1074/jbc.M412819200. PMID 15637055.
- ↑ 28.0 28.1 "Identification of the protein-protein contact site and interaction mode of human VDAC1 with Bcl-2 family proteins". Biochemical and Biophysical Research Communications 305 (4): 989–996. June 2003. doi:10.1016/s0006-291x(03)00871-4. PMID 12767928.
- ↑ "BH4 domain of antiapoptotic Bcl-2 family members closes voltage-dependent anion channel and inhibits apoptotic mitochondrial changes and cell death". Proceedings of the National Academy of Sciences of the United States of America 97 (7): 3100–3105. March 2000. doi:10.1073/pnas.97.7.3100. PMID 10737788. Bibcode: 2000PNAS...97.3100S.
- ↑ "Bcl-2 family proteins regulate the release of apoptogenic cytochrome c by the mitochondrial channel VDAC". Nature 399 (6735): 483–487. June 1999. doi:10.1038/20959. PMID 10365962. Bibcode: 1999Natur.399..483S.
- ↑ "Voltage-dependent anion-selective channel (VDAC) interacts with the dynein light chain Tctex1 and the heat-shock protein PBP74". The International Journal of Biochemistry & Cell Biology 34 (9): 1059–1070. September 2002. doi:10.1016/s1357-2725(02)00026-2. PMID 12009301.
- ↑ "Human gelsolin prevents apoptosis by inhibiting apoptotic mitochondrial changes via closing VDAC". Oncogene 19 (42): 4807–4814. October 2000. doi:10.1038/sj.onc.1203868. PMID 11039896.
- ↑ "Protein kinase Cepsilon interacts with and inhibits the permeability transition pore in cardiac mitochondria". Circulation Research 92 (8): 873–880. May 2003. doi:10.1161/01.RES.0000069215.36389.8D. PMID 12663490.
- ↑ "Voltage-dependent anion channels (VDACs) recruit Parkin to defective mitochondria to promote mitochondrial autophagy". The Journal of Biological Chemistry 287 (48): 40652–40660. November 2012. doi:10.1074/jbc.M112.419721. PMID 23060438.
- ↑ "ATP transport through VDAC and the VDAC-tubulin complex probed by equilibrium and nonequilibrium MD simulations". Biochemistry 52 (51): 9246–9256. December 2013. doi:10.1021/bi4011495. PMID 24245503.
- ↑ "Glycogen synthase kinase 3-mediated voltage-dependent anion channel phosphorylation controls outer mitochondrial membrane permeability during lipid accumulation". Hepatology 57 (1): 93–102. January 2013. doi:10.1002/hep.25967. PMID 22814966.
Further reading
- "Study of PTPC composition during apoptosis for identification of viral protein target". Annals of the New York Academy of Sciences 1010 (1): 126–142. December 2003. doi:10.1196/annals.1299.022. PMID 15033708. Bibcode: 2003NYASA1010..126V. https://hal.archives-ouvertes.fr/hal-03034595/file/FVerrier_AnnAcadSciNY_03%20%281%29.pdf.
- "Isolation of the mitochondrial benzodiazepine receptor: association with the voltage-dependent anion channel and the adenine nucleotide carrier". Proceedings of the National Academy of Sciences of the United States of America 89 (8): 3170–3174. April 1992. doi:10.1073/pnas.89.8.3170. PMID 1373486. Bibcode: 1992PNAS...89.3170M.
- "Treatment of Haemophilus aphrophilus endocarditis with ciprofloxacin". The Journal of Infection 24 (3): 317–320. May 1992. doi:10.1016/S0163-4453(05)80037-4. PMID 1602151.
- "Studies on human porin. IV. The primary structures of "Porin 31HM" purified from human skeletal muscle membranes and of "Porin 31HL" derived from human B lymphocyte membranes are identical". Biological Chemistry Hoppe-Seyler 372 (7): 455–463. July 1991. doi:10.1515/bchm3.1991.372.2.455. PMID 1657034.
- "[Identification of human porins. II. Characterization and primary structure of a 31-lDa porin from human B lymphocytes (Porin 31HL)]". Biological Chemistry Hoppe-Seyler 370 (12): 1265–1278. December 1989. PMID 2559745.
- "In vitro complex formation between the octamer of mitochondrial creatine kinase and porin". The Journal of Biological Chemistry 269 (44): 27640–27644. November 1994. doi:10.1016/S0021-9258(18)47033-0. PMID 7525559.
- "Subcellular localization of human voltage-dependent anion channel isoforms". The Journal of Biological Chemistry 270 (23): 13998–14006. June 1995. doi:10.1074/jbc.270.23.13998. PMID 7539795.
- "Mapping of residues forming the voltage sensor of the voltage-dependent anion-selective channel". Proceedings of the National Academy of Sciences of the United States of America 90 (12): 5446–5449. June 1993. doi:10.1073/pnas.90.12.5446. PMID 7685903. Bibcode: 1993PNAS...90.5446T.
- "Cloning and functional expression in yeast of two human isoforms of the outer mitochondrial membrane channel, the voltage-dependent anion channel". The Journal of Biological Chemistry 268 (3): 1835–1841. January 1993. doi:10.1016/S0021-9258(18)53930-2. PMID 8420959.
- "Human mitochondrial import receptor, Tom20p. Use of glutathione to reveal specific interactions between Tom20-glutathione S-transferase and mitochondrial precursor proteins". FEBS Letters 404 (2–3): 314–318. March 1997. doi:10.1016/S0014-5793(97)00145-2. PMID 9119086.
- "Endosomes: another extra-mitochondrial location of type-1 porin/voltage-dependent anion-selective channels". Pflügers Archiv 436 (3): 478–480. August 1998. doi:10.1007/s004240050659. PMID 9644232.
- "Bax interacts with the permeability transition pore to induce permeability transition and cytochrome c release in isolated mitochondria". Proceedings of the National Academy of Sciences of the United States of America 95 (25): 14681–14686. December 1998. doi:10.1073/pnas.95.25.14681. PMID 9843949. Bibcode: 1998PNAS...9514681N.
- "Cyclophilin-D binds strongly to complexes of the voltage-dependent anion channel and the adenine nucleotide translocase to form the permeability transition pore". European Journal of Biochemistry 258 (2): 729–735. December 1998. doi:10.1046/j.1432-1327.1998.2580729.x. PMID 9874241.
- "Mapping of the human Voltage-Dependent Anion Channel isoforms 1 and 2 reconsidered". Biochemical and Biophysical Research Communications 255 (3): 707–710. February 1999. doi:10.1006/bbrc.1998.0136. PMID 10049775.
- "Bcl-2 family proteins regulate the release of apoptogenic cytochrome c by the mitochondrial channel VDAC". Nature 399 (6735): 483–487. June 1999. doi:10.1038/20959. PMID 10365962. Bibcode: 1999Natur.399..483S.
- "Revised fine mapping of the human voltage-dependent anion channel loci by radiation hybrid analysis". Mammalian Genome 10 (10): 1041–1042. October 1999. doi:10.1007/s003359901158. PMID 10501981.
- "The HIV-1 viral protein R induces apoptosis via a direct effect on the mitochondrial permeability transition pore". The Journal of Experimental Medicine 191 (1): 33–46. January 2000. doi:10.1084/jem.191.1.33. PMID 10620603.
- "Proapoptotic BH3-only Bcl-2 family members induce cytochrome c release, but not mitochondrial membrane potential loss, and do not directly modulate voltage-dependent anion channel activity". Proceedings of the National Academy of Sciences of the United States of America 97 (2): 577–582. January 2000. doi:10.1073/pnas.97.2.577. PMID 10639121. Bibcode: 2000PNAS...97..577S.
- "Mitochondria-derived and extra-mitochondrial human type-1 porin are identical as revealed by amino acid sequencing and electrophysiological characterisation". Biological Chemistry 380 (12): 1461–1466. December 1999. doi:10.1515/BC.1999.189. PMID 10661876.
External links
- VDAC1+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH)
- Overview of all the structural information available in the PDB for UniProt: P21796 (Voltage-dependent anion-selective channel protein 1) at the PDBe-KB.
![]() | Original source: https://en.wikipedia.org/wiki/VDAC1.
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