Biology:ASIC2
 Generic protein structure example |
Acid-sensing ion channel 2 (ASIC2) also known as amiloride-sensitive cation channel 1, neuronal (ACCN1) or brain sodium channel 1 (BNaC1) is a protein that in humans is encoded by the ASIC2 gene. The ASIC2 gene is one of the five paralogous genes that encode proteins that form trimeric acid-sensing ion channels (ASICs) in mammals.[1] The cDNA of this gene was first cloned in 1996.[2][3][4][5] The ASIC genes have splicing variants that encode different proteins that are called isoforms.
These genes are mainly expressed in the central and peripheral nervous system.
ASICs can form both homotrimeric (meaning composed of three identical subunits) and heterotrimeric channels.[6][7]
Structure and function
This gene encodes a member of the ASIC/ENaC superfamily of proteins.[8] The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane (TM) regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The TM regions are generally symbolized as TM1 (clone to N-terminus) and TM2 (close to C-terminus).
The pore of the channel through which ions selectively flow from the extracellular side into the cytoplasm is formed by the three TM2 regions of the trimer.[1]
References
- ↑ 1.0 1.1 "ASIC and ENaC type sodium channels: Conformational states and the structures of the ion selectivity filters". FEBS Journal 284 (4): 525–545. 2017. doi:10.1111/febs.13840. PMID 27580245. https://zenodo.org/record/890906.
- ↑ "The human degenerin MDEG, an amiloride-sensitive neuronal cation channel, is localized on chromosome 17q11.2-17q12 close to the microsatellite D17S798". Genomics 37 (2): 269–70. Oct 1996. doi:10.1006/geno.1996.0558. PMID 8921408.
- ↑ "Cloning and expression of a novel human brain Na+ channel". The Journal of Biological Chemistry 271 (14): 7879–82. Apr 1996. doi:10.1074/jbc.271.14.7879. PMID 8626462.
- ↑ "The mammalian degenerin MDEG, an amiloride-sensitive cation channel activated by mutations causing neurodegeneration in Caenorhabditis elegans". The Journal of Biological Chemistry 271 (18): 10433–6. May 1996. doi:10.1074/jbc.271.18.10433. PMID 8631835.
- ↑ "BNaC1 and BNaC2 constitute a new family of human neuronal sodium channels related to degenerins and epithelial sodium channels". Proceedings of the National Academy of Sciences of the United States of America 94 (4): 1459–64. Feb 1997. doi:10.1073/pnas.94.4.1459. PMID 9037075. Bibcode: 1997PNAS...94.1459G.
- ↑ "Mammalian ASIC2a and ASIC3 subunits co-assemble into heteromeric proton-gated channels sensitive to Gd3+". The Journal of Biological Chemistry 275 (37): 28519–25. Sep 2000. doi:10.1074/jbc.M004114200. PMID 10842183.
- ↑ "The acid-sensitive ionic channel subunit ASIC and the mammalian degenerin MDEG form a heteromultimeric H+-gated Na+ channel with novel properties". The Journal of Biological Chemistry 272 (46): 28819–22. Nov 1997. doi:10.1074/jbc.272.46.28819. PMID 9360943.
- ↑ "Epithelial sodium channel (ENaC) family: Phylogeny, structure-function, tissue distribution, and associated inherited diseases.". Gene 579 (2): 95–132. Jan 2016. doi:10.1016/j.gene.2015.12.061. PMID 26772908.
Further reading
- "The PDZ domain protein PICK1 and the sodium channel BNaC1 interact and localize at mechanosensory terminals of dorsal root ganglion neurons and dendrites of central neurons". The Journal of Biological Chemistry 277 (7): 5203–8. Feb 2002. doi:10.1074/jbc.M104748200. PMID 11739374.
- "Interaction of the synaptic protein PICK1 (protein interacting with C kinase 1) with the non-voltage gated sodium channels BNC1 (brain Na+ channel 1) and ASIC (acid-sensing ion channel)". The Biochemical Journal 361 (Pt 3): 443–50. Feb 2002. doi:10.1042/0264-6021:3610443. PMID 11802773.
- "Stomatin modulates gating of acid-sensing ion channels". The Journal of Biological Chemistry 279 (51): 53886–91. Dec 2004. doi:10.1074/jbc.M407708200. PMID 15471860.
- "Analysis of the membrane topology of the acid-sensing ion channel 2a". The Journal of Biological Chemistry 279 (53): 55514–9. Dec 2004. doi:10.1074/jbc.M411849200. PMID 15504740.
- "Surface expression of ASIC2 inhibits the amiloride-sensitive current and migration of glioma cells". The Journal of Biological Chemistry 281 (28): 19220–32. Jul 2006. doi:10.1074/jbc.M603100200. PMID 16704974.
- "A kinase-anchoring protein 150 and calcineurin are involved in regulation of acid-sensing ion channels ASIC1a and ASIC2a". The Journal of Biological Chemistry 282 (31): 22668–77. Aug 2007. doi:10.1074/jbc.M703624200. PMID 17548344.
- "Parkin-mediated monoubiquitination of the PDZ protein PICK1 regulates the activity of acid-sensing ion channels". Molecular Biology of the Cell 18 (8): 3105–18. Aug 2007. doi:10.1091/mbc.E05-11-1027. PMID 17553932.
External links
- Human ASIC2 genome location and ASIC2 gene details page in the UCSC Genome Browser.
- Human BNC1 genome location and BNC1 gene details page in the UCSC Genome Browser.
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