Biology:CD43
 Generic protein structure example |
Leukosialin also known as sialophorin or CD43 (cluster of differentiation 43) is a transmembrane cell surface protein that in humans is encoded by the SPN (sialophorin) gene.[1][2][3]
Function
Sialophorin (leukosialin) is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which appears to be important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation.[1]
Clinical significance
Defects in the CD43 molecule are associated with the development of Wiskott–Aldrich syndrome.[4] It also appears in about 25% of intestinal MALTomas.[citation needed] Using immunohistochemistry, CD43 can be demonstrated in the paracortical T-cells of healthy lymph nodes and tonsils; it is also positive in a range of lymphoid and myeloid tumours. Although it is present in over 90% of T-cell lymphomas, it is generally less effective at demonstrating this condition than is CD3 antigen. However, it may be useful as part of a panel to demonstrate B-cell lymphoblastic lymphoma, since the malignant cells in this condition are often CD43 positive, and may be difficult to stain with other antibodies. Because it stains granulocytes and their precursors, it is also an effective marker for myeloid tumours.[5]
Interactions
CD43 has been shown to interact with EZR[6] and Moesin.[6][7]
References
- ↑ 1.0 1.1 "Entrez Gene: SPN sialophorin (leukosialin, CD43)". https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=6693.
- ↑ "Characterization of cDNAs encoding human leukosialin and localization of the leukosialin gene to chromosome 16". Proc. Natl. Acad. Sci. U.S.A. 86 (4): 1328–32. February 1989. doi:10.1073/pnas.86.4.1328. PMID 2521952. Bibcode: 1989PNAS...86.1328P.
- ↑ "Molecular characterization of sialophorin (CD43), the lymphocyte surface sialoglycoprotein defective in Wiskott-Aldrich syndrome". Proc. Natl. Acad. Sci. U.S.A. 86 (8): 2819–23. April 1989. doi:10.1073/pnas.86.8.2819. PMID 2784859. Bibcode: 1989PNAS...86.2819S.
- ↑ "Sialophorin (CD43) and the Wiskott–Aldrich syndrome". Immunodefic Rev 2 (2): 151–74. 1990. PMID 2223062.
- ↑ Leong, Anthony S-Y; Cooper, Kumarason; Leong, F Joel W-M (2003). Manual of Diagnostic Cytology (2nd ed.). Greenwich Medical Media, Ltd.. p. 113. ISBN 978-1-84110-100-2.
- ↑ 6.0 6.1 "CD43 interacts with moesin and ezrin and regulates its redistribution to the uropods of T lymphocytes at the cell-cell contacts". Blood 91 (12): 4632–44. June 1998. doi:10.1182/blood.V91.12.4632. PMID 9616160.
- ↑ "Ezrin/radixin/moesin (ERM) proteins bind to a positively charged amino acid cluster in the juxta-membrane cytoplasmic domain of CD44, CD43, and ICAM-2". J. Cell Biol. 140 (4): 885–95. February 1998. doi:10.1083/jcb.140.4.885. PMID 9472040.
Further reading
- "Leukosialin, a major sialoglycoprotein on human leukocytes as differentiation antigens.". Med. Biol. 64 (6): 335–43. 1987. PMID 2950285.
- "Unusual variability of the complex dinucleotide repeat block at the SPN locus.". Hum. Mol. Genet. 1 (8): 657. 1993. doi:10.1093/hmg/1.8.657. PMID 1301183.
- "CD43, a molecule defective in Wiskott-Aldrich syndrome, binds ICAM-1.". Nature 354 (6350): 233–5. 1992. doi:10.1038/354233a0. PMID 1683685. Bibcode: 1991Natur.354..233R.
- "Amino acid sequence of human plasma galactoglycoprotein: identity with the extracellular region of CD43 (sialophorin).". Proc. Natl. Acad. Sci. U.S.A. 89 (2): 663–7. 1992. doi:10.1073/pnas.89.2.663. PMID 1731338. Bibcode: 1992PNAS...89..663S.
- "A short, novel promoter sequence confers the expression of human leukosialin, a major sialoglycoprotein on leukocytes.". J. Biol. Chem. 266 (13): 8483–9. 1991. doi:10.1016/S0021-9258(18)93000-0. PMID 1827122.
- "Enhancement of T-cell activation by the CD43 molecule whose expression is defective in Wiskott-Aldrich syndrome.". Nature 350 (6320): 706–9. 1991. doi:10.1038/350706a0. PMID 2023632. Bibcode: 1991Natur.350..706P.
- "Structure of the human sialophorin (CD43) gene. Identification of features atypical of genes encoding integral membrane proteins.". Biochem. J. 270 (3): 569–76. 1990. doi:10.1042/bj2700569. PMID 2241892.
- "Enhanced susceptibility to human immunodeficiency virus infection in CD4+ T lymphocytes genetically deficient in CD43.". AIDS Res. Hum. Retroviruses 11 (9): 1015–21. 1996. doi:10.1089/aid.1995.11.1015. PMID 8554898.
- "Regulation mechanism of ERM (ezrin/radixin/moesin) protein/plasma membrane association: possible involvement of phosphatidylinositol turnover and Rho-dependent signaling pathway.". J. Cell Biol. 135 (1): 37–51. 1996. doi:10.1083/jcb.135.1.37. PMID 8858161.
- "Ezrin/radixin/moesin (ERM) proteins bind to a positively charged amino acid cluster in the juxta-membrane cytoplasmic domain of CD44, CD43, and ICAM-2.". J. Cell Biol. 140 (4): 885–95. 1998. doi:10.1083/jcb.140.4.885. PMID 9472040.
- "T cell activation through the CD43 molecule leads to Vav tyrosine phosphorylation and mitogen-activated protein kinase pathway activation.". J. Biol. Chem. 273 (23): 14218–24. 1998. doi:10.1074/jbc.273.23.14218. PMID 9603925.
- "CD43 interacts with moesin and ezrin and regulates its redistribution to the uropods of T lymphocytes at the cell-cell contacts.". Blood 91 (12): 4632–44. 1998. doi:10.1182/blood.V91.12.4632. PMID 9616160.
- "Restricted receptor segregation into membrane microdomains occurs on human T cells during apoptosis induced by galectin-1.". J. Immunol. 163 (7): 3801–11. 1999. doi:10.4049/jimmunol.163.7.3801. PMID 10490978.
- "Neutrophil polarity and locomotion are associated with surface redistribution of leukosialin (CD43), an antiadhesive membrane molecule.". Blood 95 (8): 2462–70. 2000. doi:10.1182/blood.V95.8.2462. PMID 10753822.
- "A macrophage invasion mechanism for mycobacteria implicating the extracellular domain of CD43.". J. Exp. Med. 192 (2): 183–92. 2000. doi:10.1084/jem.192.2.183. PMID 10899905.
- "CD43-mediated signals induce DNA binding activity of AP-1, NF-AT, and NFkappa B transcription factors in human T lymphocytes.". J. Biol. Chem. 275 (40): 31460–8. 2000. doi:10.1074/jbc.M005231200. PMID 10908570.
- "Cutting edge: CD43 functions as a T cell counterreceptor for the macrophage adhesion receptor sialoadhesin (Siglec-1).". J. Immunol. 166 (6): 3637–40. 2001. doi:10.4049/jimmunol.166.6.3637. PMID 11238599.
External links
- CD43+antigen at the US National Library of Medicine Medical Subject Headings (MeSH)
- Human SPN genome location and SPN gene details page in the UCSC Genome Browser.
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