Biology:JAG1

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Short description: Protein-coding gene in the species Homo sapiens


A representation of the 3D structure of the protein myoglobin showing turquoise α-helices.
Generic protein structure example

Jagged1 (JAG1) is one of five cell surface proteins (ligands) that interact with four receptors in the mammalian Notch signaling pathway. The Notch Signaling Pathway is a highly conserved pathway that functions to establish and regulate cell fate decisions in many organ systems. Once the JAG1-NOTCH (receptor-ligand) interactions take place, a cascade of proteolytic cleavages is triggered resulting in activation of the transcription for downstream target genes. Located on human chromosome 20, the JAG1 gene is expressed in multiple organ systems in the body and causes the autosomal dominant disorder Alagille syndrome (ALGS) resulting from loss of function mutations within the gene. JAG1 has also been designated as CD339 (cluster of differentiation 339).

Structure and function

JAG1 was first identified as a ligand that was able to activate notch receptors when it was cloned in rat in 1995.[1][2] The structure of the JAG1 protein includes a small intracellular component, a transmembrane motif, proceeded by an extracellular region containing a cystine-rich region, 16 EGF-like repeats, a DSL domain, and finally a signal peptide totaling 1218 amino acids in length over 26 coding exons.[3]

The JAG1 protein encoded by JAG1 is the human homolog of the Drosophila jagged protein.[1] Human JAG1 is one of five ligands for receptors in the NOTCH signaling pathway which helps to determine cellular fate and is active during many developmental stages. The extracellular component of the JAG1 protein physically interacts with its respective Notch receptor. This interaction kicks off a cascade of proteolytic cleavages leading to the original NOTCH intracellular domain being trafficked into the nucleus of the cell leading to the activation of different target genes.[4][5][6][7]

Expression profile and mouse studies

In situ hybridization and conditional gene knockout studies have helped to demonstrate the role JAG1 plays in development and its effects on different organ systems. In humans, JAG1 has broad expression in many tissue types including the pancreas, heart, placenta, prostate, lung, kidney, thymus, testis, and leucocytes in the adult.[8] In a developing embryo JAG1 expression is concentrated around the pulmonary artery, mesocardium, distal cardic outflow tract, major arteries, metanephros, branchial arches, pancreas, the portal vein, and otocyst.[8] Generally, JAG1 expression patterns correlate with organ systems affected in ALGS, although it is important to note that not all tissues where JAG1 is expressed are affected in ALGS. More recently JAG1 expression has been found to be altered in breast cancer and adrenocortical carcinoma patients.[9][10]

Mouse models where the Jag1 gene is turned off in certain tissues (conditional knockout mouse models) have been used to study the role of Jag1 in many tissue specific areas. While homozygous deletions of Jag1 have been shown to be embryonic lethal in mice, and heterozygous deletions may show only a limited phenotype (involving the eye), mice haploinsufficient for both Jag1 and Notch2 present with the ALGS phenotype.[11] Conditional gene knockout mouse models with Jag1 mutations targeted to the portal vein mesenchyme, endothelium, and cranial neural crest all exhibit features classic to those in individuals with ALGS, highlighting the role of this tissue type in disease origins[12][13][14][15][16]

Disease phenotype

ALGS is an autosomal dominant multi-system disorder affecting several body systems including the liver, heart, skeleton, eye, facial structure, kidneys and vascular system. The most clinically significant concerns stem from liver, heart, vascular or renal problems. Mutations in JAG1 were first discovered to be responsible for ALGS by researchers at The Children's Hospital of Philadelphia and the National Institutes of Health in 1997.[2] Patients who are clinically consistent with the disorder usually have a mutation in JAG1 (94%), while a smaller 2% have a mutation in NOTCH2.[17] Over half of individuals with mutations in the gene did not inherit it from either parent, and thus have a de novo mutation.[17][18] JAG1 mutation types include protein truncating (splice site, frameshift, and nonsense), missense, and whole gene deletions accounting for 80%, 7%, and 12% respectively. Since all mutation types lead to a patient phenotype, it is thought that haploinsufficiency for JAG1 is the likely disease mechanism of action.[19][20][21] Although individuals can have a range of mutation types in JAG1, all of the known mutations lead to loss of the function of one copy, and, there is no correlation between mutation type or location and disease severity. Though individuals with ALGS have several body systems affected, there is a subset of individuals with JAG1 mutations who present with tetralogy of fallot/pulmonary stenosis that do not show the other clinical signs of the syndrome.[22] Given the variable expressivity of the disease, there may be other genetic or environmental modifiers present beyond the original JAG1 mutation.

More recently, JAG1 expression changes have been implicated in many types of cancer. Specifically, up regulation of JAG1 has been correlated with both poor overall breast cancer survival rates and an enhancement of tumor proliferation in adrenocortical carcinoma patients.[9][23][24][25]

See also

Notes

References

  1. ↑ 1.0 1.1 "Jagged: a mammalian ligand that activates Notch1". Cell 80 (6): 909–17. 1995. doi:10.1016/0092-8674(95)90294-5. PMID 7697721. 
  2. ↑ 2.0 2.1 "Mutations in the human Jagged1 gene are responsible for Alagille syndrome". Nat. Genet. 16 (3): 235–42. 1997. doi:10.1038/ng0797-235. PMID 9207787. https://zenodo.org/record/1233393. 
  3. ↑ "Exon 6 of human Jagged-1 encodes an autonomously folding unit". FEBS Lett. 574 (1–3): 156–60. 2004. doi:10.1016/j.febslet.2004.08.022. PMID 15358557. 
  4. ↑ "Physical interaction of Delta1, Jagged1, and Jagged2 with Notch1 and Notch3 receptors". Biochem. Biophys. Res. Commun. 276 (1): 385–9. 2000. doi:10.1006/bbrc.2000.3469. PMID 11006133. 
  5. ↑ "Manic fringe and lunatic fringe modify different sites of the Notch2 extracellular region, resulting in different signaling modulation". J. Biol. Chem. 276 (28): 25753–8. July 2001. doi:10.1074/jbc.M103473200. PMID 11346656. 
  6. ↑ "Mouse jagged1 physically interacts with notch2 and other notch receptors. Assessment by quantitative methods". J. Biol. Chem. 274 (46): 32961–9. November 1999. doi:10.1074/jbc.274.46.32961. PMID 10551863. 
  7. ↑ "Binding of Delta1, Jagged1, and Jagged2 to Notch2 Rapidly Induces Cleavage, Nuclear Translocation, and Hyperphosphorylation of Notch2". Mol. Cell. Biol. 20 (18): 6913–22. September 2000. doi:10.1128/MCB.20.18.6913-6922.2000. PMID 10958687. 
  8. ↑ 8.0 8.1 "JAGGED1 expression in human embryos: correlation with the Alagille syndrome phenotype". J. Med. Genet. 37 (9): 658–62. 2000. doi:10.1136/jmg.37.9.658. PMID 10978356. 
  9. ↑ 9.0 9.1 "High-level coexpression of JAG1 and NOTCH1 is observed in human breast cancer and is associated with poor overall survival". Cancer Res. 65 (18): 8530–7. 2005. doi:10.1158/0008-5472.CAN-05-1069. PMID 16166334. 
  10. ↑ "Upregulated JAG1 enhances cell proliferation in adrenocortical carcinoma". Clin. Cancer Res. 18 (9): 2452–64. 2012. doi:10.1158/1078-0432.CCR-11-2371. PMID 22427350. 
  11. ↑ "A mouse model of Alagille syndrome: Notch2 as a genetic modifier of Jag1 haploinsufficiency". Development 129 (4): 1075–82. 2002. doi:10.1242/dev.129.4.1075. PMID 11861489. 
  12. ↑ "The expression of Jagged1 in the developing mammalian heart correlates with cardiovascular disease in Alagille syndrome". Hum. Mol. Genet. 8 (13): 2443–9. 1999. doi:10.1093/hmg/8.13.2443. PMID 10556292. 
  13. ↑ "Endothelial deletion of murine Jag1 leads to valve calcification and congenital heart defects associated with Alagille syndrome". Development 139 (23): 4449–60. 2012. doi:10.1242/dev.084871. PMID 23095891. 
  14. ↑ "Jagged1 in the portal vein mesenchyme regulates intrahepatic bile duct development: insights into Alagille syndrome". Development 137 (23): 4061–72. 2010. doi:10.1242/dev.052118. PMID 21062863. 
  15. ↑ "Endothelial expression of the Notch ligand Jagged1 is required for vascular smooth muscle development". Proc. Natl. Acad. Sci. U.S.A. 105 (6): 1955–9. 2008. doi:10.1073/pnas.0709663105. PMID 18245384. Bibcode: 2008PNAS..105.1955H. 
  16. ↑ "Cranial neural crest ablation of Jagged1 recapitulates the craniofacial phenotype of Alagille syndrome patients". Hum. Mol. Genet. 21 (6): 1374–83. 2012. doi:10.1093/hmg/ddr575. PMID 22156581. 
  17. ↑ 17.0 17.1 "Spectrum and frequency of jagged1 (JAG1) mutations in Alagille syndrome patients and their families". Am. J. Hum. Genet. 62 (6): 1361–9. 1998. doi:10.1086/301875. PMID 9585603. 
  18. ↑ "Jagged1 (JAG1) mutations in Alagille syndrome: increasing the mutation detection rate". Hum. Mutat. 27 (5): 436–43. 2006. doi:10.1002/humu.20310. PMID 16575836. 
  19. ↑ "Notch signaling in human development and disease". Semin. Cell Dev. Biol. 23 (4): 450–7. 2012. doi:10.1016/j.semcdb.2012.01.010. PMID 22306179. 
  20. ↑ "Defective intracellular transport and processing of JAG1 missense mutations in Alagille syndrome". Hum. Mol. Genet. 10 (4): 405–13. 2001. doi:10.1093/hmg/10.4.405. PMID 11157803. 
  21. ↑ "Mutations in JAGGED1 gene are predominantly sporadic in Alagille syndrome". Gastroenterology 116 (5): 1141–8. 1999. doi:10.1016/S0016-5085(99)70017-X. PMID 10220506. 
  22. ↑ "Jagged1 (JAG1) mutations in patients with tetralogy of Fallot or pulmonic stenosis". Hum. Mutat. 31 (5): 594–601. 2010. doi:10.1002/humu.21231. PMID 20437614. 
  23. ↑ "High-level JAG1 mRNA and protein predict poor outcome in breast cancer". Mod. Pathol. 20 (6): 685–93. 2007. doi:10.1038/modpathol.3800785. PMID 17507991. 
  24. ↑ "The notch ligand JAGGED1 as a target for anti-tumor therapy". Front Oncol 4: 254. 2014. doi:10.3389/fonc.2014.00254. PMID 25309874. 
  25. ↑ "Gene alterations identified by expression profiling in tumor-associated endothelial cells from invasive ovarian carcinoma". Cancer Res. 67 (4): 1757–68. 2007. doi:10.1158/0008-5472.CAN-06-3700. PMID 17308118. 

Further reading

External links

This article incorporates text from the United States National Library of Medicine, which is in the public domain.



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