Biology:FCAR

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Short description: Mammalian protein found in Homo sapiens


A representation of the 3D structure of the protein myoglobin showing turquoise α-helices.
Generic protein structure example

Fc fragment of IgA receptor (FCAR) is a human gene[1] that codes for the transmembrane receptor FcαRI, also known as CD89 (Cluster of Differentiation 89). FcαRI binds the heavy-chain constant region of Immunoglobulin A (IgA) antibodies.[2] FcαRI is present on the cell surface of myeloid lineage cells, including neutrophils, monocytes, macrophages, and eosinophils,[3] though it is notably absent from intestinal macrophages[4] and does not appear on mast cells.[3] FcαRI plays a role in both pro- and anti-inflammatory responses depending on the state of IgA bound.[3] Inside-out signaling primes FcαRI in order for it to bind its ligand,[2] while outside-in signaling caused by ligand binding depends on FcαRI association with the Fc receptor gamma chain (FcR γ-chain).[3]

Though FcαRI is part of the Fc receptor immunoglobulin superfamily, the protein's primary structure is similar to receptors in the leukocyte receptor cluster (LRC), and the FCAR gene appears amidst LRC genes on chromosome 19.[2][3] This contrasts with the location of other members of the Fc receptor immunoglobulin superfamily, which are encoded on chromosome 1.[2][3] Additionally, though there are equivalents to FCAR in several species, there is no such homolog in mice.[2]

Structure

The FcαRI α-chain consists of two extracellular domains, EC1 and EC2, at a right angle to each other, a transmembrane domain, and an intracellular domain.[2] However, this chain alone cannot perform signaling in response to IgA binding, and FcαRI must associate with a dimeric form of FcR g-chain, the ends of which contain immunoreceptor tyrosine-based activation motifs (ITAMs). The FcR γ-chain is responsible for relaying the signal to the inside of the cell.[2][3]

Two FCAR alleles differing by a single nucleotide polymorphism (SNP) code for two FcαRI molecules that differ in their ability to signal for IL-6 and TNF-α production and release.[5] The SNP results in either serine or glycine as the 248th residue of the amino acid sequence, a position in the intracellular domain of FcαRI.[5] Compared to FcαRI with Ser248, FcαRI molecules with Gly248 are better able to signal for the release of IL-6, even independently from FcR γ-chain association.[5]

Alternative splicing of the transcript from this gene produces ten mRNA variants encoding different isoforms.[1]

Inside-Out Signaling

FcαRI must first be primed by a process called inside-out signaling in order to bind with increased ability to IgA. Priming occurs when cytokines signaling the presence of an infection bind their receptors on FcαRI-expressing cells, activating the kinase PI3K. PI3K then activates p38 and PKC, which together with PP2A lead to the dephosphorylation of the Serine 263 residue (Ser263) on the intracellular domain of the FcαRI α-chain.[6] The priming of FcαRI to be able to bind IgA does not depend on FcαRI association with the FcR γ-chain,[3] but does depend on cytoskeleton organization.[6]

Once primed, FcαRI can bind IgA.[6] The FcαRI EC1 domain binds the hinge between the IgA-Fc regions Ca2 and Ca3 regions.[2]

Function

Signaling and the resulting cellular response caused by FcαRI binding IgA varies depending on the state of the IgA molecules. A pro-inflammatory response is signaled when IgA molecules in an immune complex bind to multiple FcαRI, resulting in the activation of Src family kinases and the phosphorylation of the FcR γ-chain ITAMs by Lyn.[7] Syk, a tyrosine kinase, subsequently docks at the phosphorylated ITAMs and initiates PI3K and PLC-γ signaling.[7] The ensuing signaling cascades lead to pro-inflammatory responses such as release of cytokines, phagocytosis, respiratory bursts, antibody-dependent cell-mediated cytotoxicity, production of reactive oxygen species, and antigen presentation.[2][3]

Despite signaling via ITAMs, which typically initiate activation cascades, FcαRI may either act as an activating or inhibitory receptor.[8] Inhibitory ITAM signaling (ITAMi) results in anti-inflammatory responses. When FcαRI monovalently binds monomeric, non-antigen bound IgA, the form most common in serum,[2] the resulting signals result in inactivation of other activating receptors such as FcγR and FcεRI. The binding of the monomeric serum IgA causes Lyn to only partly phosphorylate the FcR γ-chain ITAMs. Consequently, Src homology region 2 domain-containing phosphatase-1 (SHP-1) is recruited by Syk to the FcR γ-chain.[7] A tyrosine phosphatase, SHP-1 coordinates the anti-inflammatory response, preventing other receptors from signaling for pro-inflammatory responses by not allowing these receptors to become phosphorylated.[7] This ITAMi signaling supports homeostasis in the absence of pathogens.[7]

The anti-inflammatory role of monomeric IgA-FcαRI binding may have implications for treatment of allergic asthma, as shown by targeting FcαRI in transgenic mice models with anti-FcαRI Fab antibodies, which mimic the binding of monomeric IgA.[9] This FcαRI targeting led to decreased infiltration of airway tissue by inflammatory leukocytes.[9]

The secreted form of IgA (sIgA), a homodimer secreted across epithelial linings such as the gut epithelium, is sterically hindered in its binding to FcαRI. This is because some of sIgA's FcαRI binding site is obscured by a section of the cleaved polymeric Ig receptor that aided sIgA's secretion into the gut lumen.[3] However, the precursor to sIgA, dimeric IgA (dIgA), binds to FcαRI with approximately the same affinity as monomeric IgA.[3] Secreted IgA plays an important role in preventing immune response to commensal gut microbes, and accordingly intestinal macrophages do not express FcαRI.[2] However, during invasion of mucosal tissue by pathogenic bacteria, neutrophils responding to the infection will bind and phagocytose dIgA-opsonized bacteria via FcαRI.[2]

FcαRI is also an important Fc receptor for neutrophil killing of tumor cells. When FcαRI-expressing neutrophils come into contact with IgA-opsonized tumor cells, the neutrophils not only perform antibody-dependent cell-mediated cytotoxicity, but also release the cytokines TNF-α and IL-1β which cause increased neutrophil migration to the site.[10]

Interactions

FCAR has been shown to interact with FCGR1A.[11]

See also

References

  1. ↑ 1.0 1.1 "Entrez Gene: FCAR Fc fragment of IgA, receptor for". https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=2204. 
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 "The human immunoglobulin A Fc receptor FcαRI: a multifaceted regulator of mucosal immunity". Mucosal Immunology 4 (6): 612–24. November 2011. doi:10.1038/mi.2011.36. PMID 21937986. 
  3. ↑ 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 "The era of the immunoglobulin A Fc receptor FcαRI; its function and potential as target in disease". Immunological Reviews 268 (1): 123–38. November 2015. doi:10.1111/imr.12337. PMID 26497517. 
  4. ↑ "Intestinal macrophages lack CD14 and CD89 and consequently are down-regulated for LPS- and IgA-mediated activities". Journal of Immunology 167 (5): 2651–6. September 2001. doi:10.4049/jimmunol.167.5.2651. PMID 11509607. 
  5. ↑ 5.0 5.1 5.2 "FcalphaRI (CD89) alleles determine the proinflammatory potential of serum IgA". Journal of Immunology 178 (6): 3973–82. March 2007. doi:10.4049/jimmunol.178.6.3973. PMID 17339498. 
  6. ↑ 6.0 6.1 6.2 "Fc receptor inside-out signaling and possible impact on antibody therapy". Immunological Reviews 268 (1): 74–87. November 2015. doi:10.1111/imr.12332. PMID 26497514. 
  7. ↑ 7.0 7.1 7.2 7.3 7.4 "Anti-inflammatory role of the IgA Fc receptor (CD89): from autoimmunity to therapeutic perspectives". Autoimmunity Reviews 12 (6): 666–9. April 2013. doi:10.1016/j.autrev.2012.10.011. PMID 23201915. 
  8. ↑ "Of ITIMs, ITAMs, and ITAMis: revisiting immunoglobulin Fc receptor signaling". Immunological Reviews 268 (1): 66–73. November 2015. doi:10.1111/imr.12336. PMID 26497513. 
  9. ↑ 9.0 9.1 "Identification of FcalphaRI as an inhibitory receptor that controls inflammation: dual role of FcRgamma ITAM". Immunity 22 (1): 31–42. January 2005. doi:10.1016/j.immuni.2004.11.017. PMID 15664157. 
  10. ↑ "Neutrophils as effector cells for antibody-based immunotherapy of cancer". Seminars in Cancer Biology 23 (3): 190–9. June 2013. doi:10.1016/j.semcancer.2012.12.002. PMID 23287459. 
  11. ↑ "Functional association between the human myeloid immunoglobulin A Fc receptor (CD89) and FcR gamma chain. Molecular basis for CD89/FcR gamma chain association". The Journal of Biological Chemistry 270 (50): 29781–7. December 1995. doi:10.1074/jbc.270.50.29781. PMID 8530370. 

Further reading

External links

This article incorporates text from the United States National Library of Medicine, which is in the public domain.



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