Chemistry:Dextrallorphan

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Short description: Chemical compound
Dextrallorphan
Dextrallorphan.svg
Clinical data
Routes of
administration		Oral
ATC code
  • none
Legal status
Legal status
  • In general: uncontrolled
Identifiers
CAS Number
PubChem CID
ChemSpider
Chemical and physical data
FormulaC19H25NO
Molar mass283.415 g·mol−1
3D model (JSmol)

Dextrallorphan (DXA) is a chemical of the morphinan class that is used in scientific research. It acts as a σ1 receptor agonist and NMDA receptor antagonist.[1][2][3][4] It has no significant affinity for the σ2, μ-opioid, or δ-opioid receptor, or for the serotonin or norepinephrine transporter.[2][5] As an NMDA receptor antagonist, in vivo, it is approximately twice as potent as dextromethorphan, and five-fold less potent than dextrorphan.[3]

Uses in Scientific Research

Masking of sigma-1 receptor

Dextrallorphan is often used in research to block σ1 receptor sites so that σ2 receptor sites (which have not been cloned yet [when?]) can be studied.[6][7][8] It was hypothesized that both of these sigma (σ) receptors were opioid receptors, due to their affinity for psychoactive drugs. However, it is now understood that they are non-opioid receptors that bind to certain psychoactive drugs, like dextrallorphan.[9] One example of dextrallorphan being used to mask σ1 receptor sites was seen in a study on the localization of the σ2 receptor in detergent-resistant lipid raft domains.[6] It has also been used to mask σ1 receptor sites so that σ2 receptor binding characteristics in the rat liver could be determined, by labeling σ2 receptor sites with [3H]l,3-di-o-tolylguanidine (DTG) in the presence of 1 μM dextrallorphan solution.[8]

Animal Studies

Dextrallorphan was used in Spraque-Dawley rats to study cerebellar Purkinje neurons electrophysical responses to the drug when it was applied iontophoretically as a sigma (σ) receptor ligand. Dextrallorphan increased the firing rate by 14%, suggesting that sigma (σ) ligands (like dextrallorphan) alter the spontaneous firing of Purkinje neurons and cause motor effects.[10]

In another study, dextrallorphan, along with other opioid derivatives, was found to be a potent inhibitor of etorphine-inaccessible (EI) sites in the guinea-pig brain. Dextrallorphan was of the top three most potent opioid inhibitors of those studied, with a concentration of 67 nM required to show 50% inhibition.[1]

History

In 1955, dextrallorphan has been used to study inhibition of cholinesterases and to look at the relationship between analgetics and acetylcholine metabolism.[11] It was found that dextrallorphan inhibits 25% of bovine erythrocyte cholinesterase at a dose of 10−3 mole/liter, which corresponds to a concentration of up to 0.2 mg/kg in dog intestine. However, at this dose the drug showed no effect on the gut tone. Dextrallorphan was classified as a potent inhibitor of the intestinal and red blood cell cholinesterase based on the concentration of the drug needed to inhibit these enzymes in the cholinesterase preparations from the animals systems utilized. Simultaneously, dextrallorphan showed no analgesia and no change in intestinal tone. With these results dextrallorphan helped proved that there is no correlation between the inhibition of cholinesterase systems and analgetic or intestinal effects.[12]

In 1979, dextrallorphan was found to have a half maximal inhibitory concentration (IC50) for binding to the pituitary and brain receptor of 10,000 ± 1000 nM and 10,000 ± 1500 nM, respectively. While its stereoisomer, levallorphan, had a 10,000 times more potent dose, thus proving that binding to these receptors is stereospecific.[13]

See also

References

  1. ↑ 1.0 1.1 "Evidence for sigma opioid receptor: binding of [3HSKF-10047 to etorphine-inaccessible sites in guinea-pig brain"]. The Journal of Pharmacology and Experimental Therapeutics 223 (2): 284–90. November 1982. PMID 6290634. http://jpet.aspetjournals.org/content/223/2/284.full.pdf. 
  2. ↑ 2.0 2.1 "Serotonin and norepinephrine uptake inhibiting activity of centrally acting analgesics: structural determinants and role in antinociception". The Journal of Pharmacology and Experimental Therapeutics 274 (3): 1263–70. September 1995. PMID 7562497. http://jpet.aspetjournals.org/content/274/3/1263.full.pdf. 
  3. ↑ 3.0 3.1 "N-methyl-D-aspartate antagonist activity of alpha- and beta-sulfallorphans". The Journal of Pharmacology and Experimental Therapeutics 280 (1): 357–65. January 1997. PMID 8996216. http://jpet.aspetjournals.org/content/280/1/357.full.pdf. 
  4. ↑ "Pharmacological evaluation of N-allynormetazocine (SKF 10,047) on the basis of its discriminative stimulus properties in the rat". The Journal of Pharmacology and Experimental Therapeutics 225 (1): 144–52. April 1983. PMID 6834266. 
  5. ↑ "Synthesis and binding characteristics of potential SPECT imaging agents for sigma-1 and sigma-2 binding sites". Journal of Medicinal Chemistry 36 (5): 566–71. March 1993. doi:10.1021/jm00057a006. PMID 8496936. 
  6. ↑ 6.0 6.1 "Sigma-2 receptors are specifically localized to lipid rafts in rat liver membranes". European Journal of Pharmacology 493 (1–3): 19–28. June 2004. doi:10.1016/j.ejphar.2004.04.005. PMID 15189760. https://zenodo.org/record/1258913. 
  7. ↑ "A sigma-1 receptor selective analogue of BD1008. A potential substitute for (+)-opioids in sigma receptor binding assays". Bioorganic & Medicinal Chemistry Letters 10 (1): 17–8. January 2000. doi:10.1016/s0960-894x(99)00590-9. PMID 10636233. 
  8. ↑ 8.0 8.1 "Differential solubilization of rat liver sigma 1 and sigma 2 receptors: retention of sigma 2 sites in particulate fractions". European Journal of Pharmacology 304 (1–3): 201–10. May 1996. doi:10.1016/0014-2999(96)00109-4. PMID 8813603. https://zenodo.org/record/1253872. 
  9. ↑ "The sigma receptor: evolution of the concept in neuropsychopharmacology". Current Neuropharmacology 3 (4): 267–80. October 2005. doi:10.2174/157015905774322516. PMID 18369400. 
  10. ↑ "Effects of sigma ligands on rat cerebellar Purkinje neuron firing: an iontophoretic study". Brain Research Bulletin 35 (4): 303–9. 1994. doi:10.1016/0361-9230(94)90106-6. PMID 7850479. 
  11. ↑ "Anti-cholinesterase activity of 1-alpha-acetylmethadol: relationship to bradycardia". General Pharmacology 10 (3): 195–200. 1979. doi:10.1016/0306-3623(79)90089-2. PMID 467958. 
  12. ↑ "The interrelationships among the central, peripheral and anticholinesterase effects of some morphinan derivatives" (pdf). The Journal of Pharmacology and Experimental Therapeutics 114 (1): 33–7. May 1955. PMID 14392568. http://jpet.aspetjournals.org/content/114/1/33.long. 
  13. ↑ "Opiate receptor binding in the pituitary gland". Brain Research 124 (1): 178–84. March 1977. doi:10.1016/0006-8993(77)90877-0. PMID 191146. 



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