Biology:GRLF1

Short description: Protein-coding gene in the species Homo sapiens

Generic protein structure example

Glucocorticoid receptor repression factor 1 (or GRF-1) is a protein that in humans is encoded by the ARHGAP35 gene (previously GRLF1). Preliminary evidence suggests that it functions to repress transcription of the glucocorticoid receptor, though this remains uncertain without further evidence.

Function

The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids.

Further reading

• "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones". DNA Research 9 (3): 99–106. Jun 2002. doi:10.1093/dnares/9.3.99. PMID 12168954. 
• "Expression of human glucocorticoid receptor gene and interaction of nuclear proteins with the transcriptional control element". The Journal of Biological Chemistry 271 (31): 18662–71. Aug 1996. doi:10.1074/jbc.271.31.18662. PMID 8702520. 
• "p190-A, a human tumor suppressor gene, maps to the chromosomal region 19q13.3 that is reportedly deleted in some gliomas". Gene 257 (1): 23–31. Oct 2000. doi:10.1016/S0378-1119(00)00387-5. PMID 11054565. 
• "Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Research 7 (6): 347–55. Dec 2000. doi:10.1093/dnares/7.6.347. PMID 11214970. 
• "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the United States of America 101 (33): 12130–5. Aug 2004. doi:10.1073/pnas.0404720101. PMID 15302935. Bibcode: 2004PNAS..10112130B. 
• "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nature Biotechnology 23 (1): 94–101. Jan 2005. doi:10.1038/nbt1046. PMID 15592455. 
• "p190 Rho-GTPase activating protein associates with plexins and it is required for semaphorin signalling". Journal of Cell Science 118 (Pt 20): 4689–700. Oct 2005. doi:10.1242/jcs.02590. PMID 16188938. http://repository.cshl.edu/22532/1/Sordella%20J%20Cell%20Sci%202005.pdf. 
• "Suppression of RhoA activity by focal adhesion kinase-induced activation of p190RhoGAP: role in regulation of endothelial permeability". The Journal of Biological Chemistry 281 (4): 2296–305. Jan 2006. doi:10.1074/jbc.M511248200. PMID 16308318. 
• "Transcriptome analysis of human gastric cancer". Mammalian Genome 16 (12): 942–54. Dec 2005. doi:10.1007/s00335-005-0075-2. PMID 16341674. 
• "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell 127 (3): 635–48. Nov 2006. doi:10.1016/j.cell.2006.09.026. PMID 17081983. 
• "Angiopoietin-1 requires p190 RhoGAP to protect against vascular leakage in vivo". The Journal of Biological Chemistry 282 (33): 23910–8. Aug 2007. doi:10.1074/jbc.M702169200. PMID 17562701. 

External links

• GRLF1+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH)
• GRLF1 Info with links in the Cell Migration Gateway

This article incorporates text from the United States National Library of Medicine, which is in the public domain.