Biology:ZNF366
 Generic protein structure example |
Zinc finger protein 366, also known as DC-SCRIPT (Dendritic cell-specific transcript), is a protein that in humans is encoded by the ZNF366 gene.[1] The ZNF366 gene was first identified in a DNA comparison study between 85 kb of Fugu rubripes sequence containing 17 genes with its homologous loci in the human draft genome.[2]
Function
In 2006, DC-SCRIPT was isolated and characterized in human monocyte-derived dendritic cells (mo-DCs).[3]
DC-SCRIPT contains a DNA-binding domain (11 C2H2 zinc (Zn) fingers), flanked by a proline-rich and an acidic region, which can interact with C-terminal-binding protein 1 (CtBP1), a global corepressor. In the immune system of both mice and humans, DC-SCRIPT was found to be specifically expressed in dendritic cells (DCs).[4]
In COS-1 cells, DC-SCRIPT was shown to interact with the estrogen receptor DNA-binding domain (ERDBD) and represses ER activity through the association with RIP140, CtBP and histone deacetylases.[5]
In DCs, DC-SCRIPT was found to be highly expressed in type one conventional DCs (cDC1s) under the control of PU.1.[6] The presence of DC-SCRIPT is important for the cDC1s lineage specification via maintaining Interferon regulatory factor 8 (IRF8) expression. The DC-SCRIPT deficient cDC1s had impaired capacity to capture and present cell-associated antigens and to secrete IL-12p40.[7]
Breast cancer
In 2010, it was shown that DC-SCRIPT can act as a coregulator of multiple nuclear receptors having opposite effects on type I vs type II NRs. DC-SCRIPT is able to repress ER and PR mediated transcription, whereas it can activate transcription mediated by RAR and PPAR. In the same study, it was shown that breast tumor tissue expresses lower levels of DC-SCRIPT than normal breast tissue from the same patient and that DC-SCRIPT mRNA expression is an independent prognostic factor for good survival of breast cancer patients with estrogen receptor- and/or progesterone receptor-positive tumors.[8]
References
- ↑ "Entrez Gene: ZNF366 zinc finger protein 366". https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=167465.
- ↑ "Fugu and human sequence comparison identifies novel human genes and conserved non-coding sequences". Gene 294 (1–2): 35–44. July 2002. doi:10.1016/S0378-1119(02)00793-X. PMID 12234665.
- ↑ "Identification and characterization of DC-SCRIPT, a novel dendritic cell-expressed member of the zinc finger family of transcriptional regulators". Journal of Immunology 176 (2): 1081–9. January 2006. doi:10.4049/jimmunol.176.2.1081. PMID 16393996.
- ↑ "Molecular characterization of the murine homologue of the DC-derived protein DC-SCRIPT". Journal of Leukocyte Biology 79 (5): 1083–91. May 2006. doi:10.1189/jlb.1005588. PMID 16522745.
- ↑ "ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases". Nucleic Acids Research 34 (21): 6126–36. 2006. doi:10.1093/nar/gkl875. PMID 17085477.
- ↑ "Transcription Factor PU.1 Promotes Conventional Dendritic Cell Identity and Function via Induction of Transcriptional Regulator DC-SCRIPT". Immunity 50 (1): 77–90.e5. January 2019. doi:10.1016/j.immuni.2018.11.010. PMID 30611612.
- ↑ "Type 1 conventional dendritic cell fate and function are controlled by DC-SCRIPT". Science Immunology 6 (58): eabf4432. April 2021. doi:10.1126/sciimmunol.abf4432. PMID 33811060.
- ↑ "DC-SCRIPT: nuclear receptor modulation and prognostic significance in primary breast cancer". Journal of the National Cancer Institute 102 (1): 54–68. January 2010. doi:10.1093/jnci/djp441. PMID 20008677.
Further reading
- "DNA cloning using in vitro site-specific recombination". Genome Research 10 (11): 1788–95. November 2000. doi:10.1101/gr.143000. PMID 11076863.
- "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Research 11 (3): 422–35. March 2001. doi:10.1101/gr.GR1547R. PMID 11230166.
- "From ORFeome to biology: a functional genomics pipeline". Genome Research 14 (10B): 2136–44. October 2004. doi:10.1101/gr.2576704. PMID 15489336.
- "The LIFEdb database in 2006". Nucleic Acids Research 34 (Database issue): D415-8. January 2006. doi:10.1093/nar/gkj139. PMID 16381901.
External links
- ZNF366+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH)
This article incorporates text from the United States National Library of Medicine, which is in the public domain.
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